Lectin-functionalized poly(glycidyl methacrylate)-block-poly(vinyldimethyl azlactone) surface supports for high avidity microbial capture

Journal Article · Tue Jan 01 00:00:00 EST 2013 · Biomacromolecules

DOI:https://doi.org/10.1021/bm4011358· OSTI ID:1115366

Hansen, Ryan R ^[1]; Hinestrosa Salazar, Juan P ^[1]; Shubert, Katherine R ^[1]; Morrell, Jennifer L. ^[2]; Pelletier, Dale A ^[1]; Messman, Jamie M ^[1]; Lokitz, Bradley S ^[1]; Retterer, Scott T ^[1]

---

1. ORNL
2. University of Tennessee, Knoxville (UTK)

Microbial exopolysaccharides (EPS) play a critical and dynamic role in shaping the interactions between microbial community members and their local environment. The capture of targeted microbes using surface immobilized lectins that recognize specific extracellular oligosaccharide moieties offers a non-destructive method for functional characterization based on EPS content. In this report, we evaluate the use of the block co-polymer, poly(glycidyl methacrylate)-block-4,4-dimethyl-2-vinylazlactone (PGMA-b-PVDMA), as a surface support for lectin-specific microbial capture. Arrays of circular polymer supports ten micron in diameter were generated on silicon substrates to provide discrete, covalent coupling sites for Triticum vulgare and Lens culinaris lectins. These supports promoted microbe adhesion and colony formation in a lectin-specific manner. Silicon posts with similar topography containing only physisorbed lectins showed significantly less activity. These results demonstrate that micropatterned PGMA-b-PVDMA supports provide a unique platform for microbial capture and screening based on EPS content by combining high avidity lectin surfaces with three-dimensional topography.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences (CNMS)
• Sponsoring Organization: USDOE Office of Science (SC)
• DOE Contract Number: DE-AC05-00OR22725
• OSTI ID: 1115366
• Journal Information: Biomacromolecules, Vol. 14, Issue 10; ISSN 1525--7797
• Country of Publication: United States
• Language: English

Related Subjects

Lectins
block co-polymers
exopolysaccharide
plant growth promoting rhizobacteria
micropatterning
cellular adhesion