Reply to: Comment on 'Neutron imaging reveals internal plant water dynamics'

Journal Article · Tue Jan 01 00:00:00 EST 2013 · Plant and Soil

DOI:https://doi.org/10.1007/s11104-013-1858-y· OSTI ID:1095150

Warren, Jeffrey ^[1]; Bilheux, Hassina Z ^[1]; Cheng, Chu-lin ^[2]; Perfect, Edmund ^[1]

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1. ORNL
2. University of Tennessee, Knoxville (UTK)

Our recent publication (Warren et al. 2013) described how pulses of deuterium oxide (D2O) or H2O combined with neutron radiography can be used to indicate root water uptake and hydraulic redistribution in maize. This technique depends on the large inherent differences in neutron cross-section between D and H atoms resulting in strong image contrast. However, as illustrated by Carminati and Zarebanadkouki (2013) there can be a change in total water content without a change in contrast simply by a change in the relative proportions of D2O and H2O. While we agree with their premise and detailed calculations (Zarebanadkouki at al. 2012, 2013), further evidence suggests that mixing of D2O and H2O did not confound evidence of hydraulic redistribution in our study.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). High Flux Isotope Reactor (HFIR)
• Sponsoring Organization: USDOE Office of Science (SC)
• DOE Contract Number: DE-AC05-00OR22725
• OSTI ID: 1095150
• Journal Information: Plant and Soil, Vol. 371, Issue 1-2; ISSN 0032--079X
• Country of Publication: United States
• Language: English