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Title: Xylanases, nucleic acids encoding them and methods for making and using them

Abstract

The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal .beta.-1,4-xylosidic linkages or endo-.beta.-1,4-glucanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.

Inventors:
;
Publication Date:
Research Org.:
DOEEE (USDOE Office of Energy Efficiency and Renewable Energy (EE) (United States))
Sponsoring Org.:
USDOE
OSTI Identifier:
1087879
Patent Number(s):
8,486,680
Application Number:
12/681,604
Assignee:
BP Corporation North America Inc. (Houston, TX); Verenium Corporation (San Diego, CA)
DOE Contract Number:  
1435-04-03-CA-70224
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Gray, Kevin A, and Dirmeier, Reinhard. Xylanases, nucleic acids encoding them and methods for making and using them. United States: N. p., 2013. Web.
Gray, Kevin A, & Dirmeier, Reinhard. Xylanases, nucleic acids encoding them and methods for making and using them. United States.
Gray, Kevin A, and Dirmeier, Reinhard. 2013. "Xylanases, nucleic acids encoding them and methods for making and using them". United States. https://www.osti.gov/servlets/purl/1087879.
@article{osti_1087879,
title = {Xylanases, nucleic acids encoding them and methods for making and using them},
author = {Gray, Kevin A and Dirmeier, Reinhard},
abstractNote = {The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal .beta.-1,4-xylosidic linkages or endo-.beta.-1,4-glucanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.},
doi = {},
url = {https://www.osti.gov/biblio/1087879}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jul 16 00:00:00 EDT 2013},
month = {Tue Jul 16 00:00:00 EDT 2013}
}

Works referenced in this record:

Using molecular techniques to identify new microbial biocatalysts
journal, June 1998


Stabilization of xylanase by random mutagenesis
journal, January 1993


Actinomadura xylanase sequences and methods of use
patent, August 1999


The Use of Forced Protein Evolution to Investigate and Improve Stability of Family 10 Xylanases: The Production Of Ca2+-Independent Stable Xylanases
journal, September 2004


Thermostable xylanase
patent, June 1999


Thermostable xylanase
patent, April 1998