Fermentation and Hydrogen Metabolism Affect Uranium Reduction by Clostridia

Gao, Weimin; Francis, Arokiasamy J.

doi:10.5402/2013/657160

Title: Fermentation and Hydrogen Metabolism Affect Uranium Reduction by Clostridia

Journal Article · Tue Jan 01 00:00:00 EST 2013 · ISRN Biotechnology

DOI:https://doi.org/10.5402/2013/657160· OSTI ID:1087522

Gao, Weimin ^[1]; Francis, Arokiasamy J. ^[2]

Center for Biosignatures Discovery Automation, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA
Division of Advanced Nuclear Engineering, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea; Environmental Sciences Department, Brookhaven National Laboratory, Upton, NY 11973, USA

Previously, it has been shown that not only is uranium reduction under fermentation condition common among clostridia species, but also the strains differed in the extent of their capability and the pH of the culture significantly affected uranium(VI) reduction. In this study, using HPLC and GC techniques, metabolic properties of those clostridial strains active in uranium reduction under fermentation conditions have been characterized and their effects on capability variance of uranium reduction discussed. Then, the relationship between hydrogen metabolism and uranium reduction has been further explored and the important role played by hydrogenase in uranium(VI) and iron(III) reduction by clostridia demonstrated. When hydrogen was provided as the headspace gas, uranium(VI) reduction occurred in the presence of whole cells of clostridia. This is in contrast to that of nitrogen as the headspace gas. Without clostridia cells, hydrogen alone could not result in uranium(VI) reduction. In alignment with this observation, it was also found that either copper(II) addition or iron depletion in the medium could compromise uranium reduction by clostridia. In the end, a comprehensive model was proposed to explain uranium reduction by clostridia and its relationship to the overall metabolism especially hydrogen (H₂) production.

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Research Organization:: Brookhaven National Lab. (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

DOE Contract Number:: AC02-98CH10886

OSTI ID:: 1087522

Report Number(s):: BNL-100701-2013-JA; R&D Project: 10198; KP1702010

Journal Information:: ISRN Biotechnology, Vol. 2013, Issue none; ISSN 2090-9403

Country of Publication:: United States

Language:: English

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