Single-molecule electrophoresis
- Los Alamos National Lab., NM (United States)
A novel method for the detection and identification of single molecules in solution has been devised, computer simulated, and experimentally achieved. The technique involves the determination of electrophoretic velocities by measuring the time required for individual molecules to travel a fixed distance between two laser beams. Computer simulations of the process were performed before-hand in order to estimate the experimental feasibility of the method and to determine the optimum values for the various experimental parameters. Examples of the use of the technique for the ultrasensitive detection and identification of rhodamine-6G, a mixture of DNA restriction fragments, and a mixture of proteins in aqueous solution are presented. 20 refs., 8 figs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 106760
- Journal Information:
- Analytical Chemistry (Washington), Vol. 67, Issue 18; Other Information: PBD: 15 Sep 1995
- Country of Publication:
- United States
- Language:
- English
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