High-Resolution Differential Ion Mobility Spectrometry of a Protein

Journal Article · Thu Jan 17 00:00:00 EST 2013 · Analytical Chemistry, 85(1):10-13

DOI:https://doi.org/10.1021/ac3029129· OSTI ID:1060109

Shvartsburg, Alexandre A; Smith, Richard D

Use of elevated electric fields and helium-rich gases has recently enabled differential IMS with resolving power up to R ~ 300. Here we applied that technique to proteins (namely, mass-selected ubiquitin ions), achieving R up to ~80 and separating many previously mixed conformers. While still limited by conformational multiplicity within each observed feature, this resolution is some four times the highest previously reported using either conventional or differential IMS. The capability for fine resolution of protein conformers may open new avenues for variant separation in top-down proteomics.

• Research Organization: Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 1060109
• Report Number(s): PNNL-SA-91957; 40072; 400412000
• Journal Information: Analytical Chemistry, 85(1):10-13, Journal Name: Analytical Chemistry, 85(1):10-13
• Country of Publication: United States
• Language: English

Related Subjects

Environmental Molecular Sciences Laboratory