Mass Spectrometry for Translational Proteomics: Progress and Clinical Implications
Mass spectrometry (MS)-based proteomics measurements have become increasingly utilized in a wide range of biological and biomedical applications, and have significantly enhanced the understanding of the complex and dynamic nature of the proteome and its connections to biology and diseases. While some MS techniques such as those for targeted analysis are increasingly applied with great success, others such as global quantitative analysis (for e.g. biomarker discovery) are more challenging and continue to be developed and refined to provide the desired throughput, sensitivity and/ or specificity. New MS capabilities and proteomics-based pipelines/strategies also keep enhancing for the advancement of clinical proteomics applications such as protein biomarker discovery and validation. Herein, we provide a brief review to summarize the current state of MS-based proteomics with respect to its advantages and present limitations, while highlighting its potential in future clinical applications.
- Research Organization:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 1050781
- Report Number(s):
- PNNL-SA-86783; 32707; 46206; 400412000; TRN: US201218%%1550
- Journal Information:
- Genome Medicine, 4:Article No. 63, Vol. 4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
60 APPLIED LIFE SCIENCES
BIOLOGY
DISEASES
DYNAMICS
MASS SPECTROSCOPY
POTENTIALS
PROTEINS
SENSITIVITY
SPECIFICITY
USES
VALIDATION
Mass spectrometry
clinical proteomics
shotgun proteomics
biomarker
targeted proteomics
selected reaction monitoring
multiple reaction monitoring
ion mobility separations.
Environmental Molecular Sciences Laboratory