First results of digital topography applied to macromolecular crystals
Journal Article
·
· Journal of Applied Crystallography
An inexpensive digital CCD camera was used to record X-ray topographs directly from large imperfect crystals of cubic insulin. The topographs recorded were not as detailed as those which can be measured with film or emulsion plates, but do show great promise. Six reflections were recorded using a set of finely spaced stills encompassing the rocking curve of each reflection. A complete topographic reflection profile could be digitally imaged in minutes. Interesting and complex internal structure was observed by this technique. The CCD chip used in the camera has anti-blooming circuitry and produced good data quality, even when pixels became overloaded.
- Research Organization:
- Brookhaven National Lab. (BNL), Upton, NY (United States)
- Sponsoring Organization:
- USDOE SC OFFICE OF BIOLOGICAL & ENVIRONMENTAL RESEARCH
- DOE Contract Number:
- DE-AC02-98CH10886
- OSTI ID:
- 1044783
- Report Number(s):
- BNL-98105-2012-JA; JACGAR; R&D Project: BO-070; KP1605010; TRN: US201214%%831
- Journal Information:
- Journal of Applied Crystallography, Vol. 37, Issue 3; ISSN 0021-8898
- Country of Publication:
- United States
- Language:
- English
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