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Title: Metabolomic Response of Human Skin Tissue to Low Dose Ionizing Radiation

Abstract

Understanding how human organs respond to ionizing radiation (IR) at a systems biology level and identifying biomarkers for IR exposure at low doses can help provide a scientific basis for establishing radiation protection standards. Little is known regarding the physiological responses to low dose IR at the metabolite level, which represents the end-point of biochemical processes inside cells. Using a full thickness human skin tissue model and GC-MS-based metabolomics analysis, we examined the metabolic perturbations at three time points (3, 24 and 48 hr) after exposure to 3, 10 and 200 cGy of X-rays. PLS-DA score plots revealed dose- and time-dependent clustering between sham and irradiated groups. Importantly, a comparable number of metabolites were detected to have significant change 48 hr after exposure to 3 and 10 cGy of irradiation, when compared with the high dose of 200 cGy. Biochemical pathway analysis showed perturbations to DNA/RNA damage and repair, lipid and energy metabolisms, even at low doses of IR.

Authors:
; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1043103
Report Number(s):
PNNL-SA-84921
30441; KP1602020; TRN: US201213%%83
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Molecular Biosystems
Additional Journal Information:
Journal Volume: 8; Journal Issue: 7
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; BIOLOGY; IONIZING RADIATIONS; IRRADIATION; LIPIDS; METABOLITES; ORGANS; RADIATION PROTECTION; REPAIR; SKIN; THICKNESS; Environmental Molecular Sciences Laboratory

Citation Formats

Hu, Zeping, Kim, Young-Mo, Sowa, Marianne B, Robinson, Robert J, Gao, Xiaoli, Metz, Thomas O, Morgan, William F, and Zhang, Qibin. Metabolomic Response of Human Skin Tissue to Low Dose Ionizing Radiation. United States: N. p., 2012. Web. doi:10.1039/C2MB25061F.
Hu, Zeping, Kim, Young-Mo, Sowa, Marianne B, Robinson, Robert J, Gao, Xiaoli, Metz, Thomas O, Morgan, William F, & Zhang, Qibin. Metabolomic Response of Human Skin Tissue to Low Dose Ionizing Radiation. United States. https://doi.org/10.1039/C2MB25061F
Hu, Zeping, Kim, Young-Mo, Sowa, Marianne B, Robinson, Robert J, Gao, Xiaoli, Metz, Thomas O, Morgan, William F, and Zhang, Qibin. 2012. "Metabolomic Response of Human Skin Tissue to Low Dose Ionizing Radiation". United States. https://doi.org/10.1039/C2MB25061F.
@article{osti_1043103,
title = {Metabolomic Response of Human Skin Tissue to Low Dose Ionizing Radiation},
author = {Hu, Zeping and Kim, Young-Mo and Sowa, Marianne B and Robinson, Robert J and Gao, Xiaoli and Metz, Thomas O and Morgan, William F and Zhang, Qibin},
abstractNote = {Understanding how human organs respond to ionizing radiation (IR) at a systems biology level and identifying biomarkers for IR exposure at low doses can help provide a scientific basis for establishing radiation protection standards. Little is known regarding the physiological responses to low dose IR at the metabolite level, which represents the end-point of biochemical processes inside cells. Using a full thickness human skin tissue model and GC-MS-based metabolomics analysis, we examined the metabolic perturbations at three time points (3, 24 and 48 hr) after exposure to 3, 10 and 200 cGy of X-rays. PLS-DA score plots revealed dose- and time-dependent clustering between sham and irradiated groups. Importantly, a comparable number of metabolites were detected to have significant change 48 hr after exposure to 3 and 10 cGy of irradiation, when compared with the high dose of 200 cGy. Biochemical pathway analysis showed perturbations to DNA/RNA damage and repair, lipid and energy metabolisms, even at low doses of IR.},
doi = {10.1039/C2MB25061F},
url = {https://www.osti.gov/biblio/1043103}, journal = {Molecular Biosystems},
number = 7,
volume = 8,
place = {United States},
year = {Fri May 18 00:00:00 EDT 2012},
month = {Fri May 18 00:00:00 EDT 2012}
}