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Title: Synchrotron-based X-ray fluorescence imaging and elemental mapping from biological samples

Abstract

The present study utilized the new hard X-ray microspectroscopy beamline facility, X27A, available at NSLS, BNL, USA, for elemental mapping. This facility provided the primary beam in a small spot of the order of {approx}10 {mu}m, for focussing. With this spatial resolution and high flux throughput, the synchrotron-based X-ray fluorescent intensities for Mn, Fe, Zn, Cr, Ti and Cu were measured using a liquid-nitrogen-cooled 13-element energy-dispersive high-purity germanium detector. The sample is scanned in a 'step-and-repeat' mode for fast elemental mapping measurements and generated elemental maps at 8, 10 and 12 keV, from a small animal shell (snail). The accumulated trace elements, from these biological samples, in small areas have been identified. Analysis of the small areas will be better suited to establish the physiology of metals in specific structures like small animal shell and the distribution of other elements.

Authors:
; ; ; ; ; ; ;
Publication Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE SC OFFICE OF SCIENCE (SC)
OSTI Identifier:
1042262
Report Number(s):
BNL-97940-2012-JA
Journal ID: ISSN 0304-4289; TRN: US201212%%673
DOE Contract Number:  
DE-AC02-98CH10886
Resource Type:
Journal Article
Journal Name:
Pramana - Journal of Physics
Additional Journal Information:
Journal Volume: 76; Journal Issue: 2; Journal ID: ISSN 0304-4289
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; ANIMALS; BEAMS; BIOLOGICAL MATERIALS; DISTRIBUTION; MULTI-ELEMENT ANALYSIS; FLUORESCENCE; GE SEMICONDUCTOR DETECTORS; IMAGES; MAPPING; MAPS; METALS; SPATIAL RESOLUTION; TRACE AMOUNTS; X-RAY FLUORESCENCE ANALYSIS

Citation Formats

Rao, D, Swapna, M, Cesareo, R, Brunetti, A, Akatsuka, T, Yuasa, T, Takeda, T, and Gigante, G. Synchrotron-based X-ray fluorescence imaging and elemental mapping from biological samples. United States: N. p., 2011. Web. doi:10.1007/s12043-011-0031-5.
Rao, D, Swapna, M, Cesareo, R, Brunetti, A, Akatsuka, T, Yuasa, T, Takeda, T, & Gigante, G. Synchrotron-based X-ray fluorescence imaging and elemental mapping from biological samples. United States. https://doi.org/10.1007/s12043-011-0031-5
Rao, D, Swapna, M, Cesareo, R, Brunetti, A, Akatsuka, T, Yuasa, T, Takeda, T, and Gigante, G. 2011. "Synchrotron-based X-ray fluorescence imaging and elemental mapping from biological samples". United States. https://doi.org/10.1007/s12043-011-0031-5.
@article{osti_1042262,
title = {Synchrotron-based X-ray fluorescence imaging and elemental mapping from biological samples},
author = {Rao, D and Swapna, M and Cesareo, R and Brunetti, A and Akatsuka, T and Yuasa, T and Takeda, T and Gigante, G},
abstractNote = {The present study utilized the new hard X-ray microspectroscopy beamline facility, X27A, available at NSLS, BNL, USA, for elemental mapping. This facility provided the primary beam in a small spot of the order of {approx}10 {mu}m, for focussing. With this spatial resolution and high flux throughput, the synchrotron-based X-ray fluorescent intensities for Mn, Fe, Zn, Cr, Ti and Cu were measured using a liquid-nitrogen-cooled 13-element energy-dispersive high-purity germanium detector. The sample is scanned in a 'step-and-repeat' mode for fast elemental mapping measurements and generated elemental maps at 8, 10 and 12 keV, from a small animal shell (snail). The accumulated trace elements, from these biological samples, in small areas have been identified. Analysis of the small areas will be better suited to establish the physiology of metals in specific structures like small animal shell and the distribution of other elements.},
doi = {10.1007/s12043-011-0031-5},
url = {https://www.osti.gov/biblio/1042262}, journal = {Pramana - Journal of Physics},
issn = {0304-4289},
number = 2,
volume = 76,
place = {United States},
year = {Sat Dec 31 00:00:00 EST 2011},
month = {Sat Dec 31 00:00:00 EST 2011}
}