An optimized colony forming assay for low-dose-radiation cell survival measurement

Journal Article · Tue Nov 01 00:00:00 EDT 2011 · International Research Journal of Biotechnology

OSTI ID:1041622

Zhu, J; Sutherland, B; Hu, W; Ding, N; Ye, C; Usikalu, M; Li, S; Hu, B; Zhou, G

The aim of this study is to develop a simple and reliable method to quantify the cell survival of low-dose irradiations. Two crucial factors were considered, the same number of cells plated in each flask and an appropriate interval between cell plating and irradiation. For the former, we optimized cell harvest with trypsin, diluted cells in one container, and directly seeded cells on the bottom of flasks in a low density before irradiation. Reproducible plating efficiency was obtained. For the latter, we plated cells on the bottom of flasks and then monitored the processing of attachment, cell cycle variations, and the plating efficiency after exposure to 20 cGy of X-rays. The results showed that a period of 4.5 h to 7.5 h after plating was suitable for further treatment. In order to confirm the reliability and feasibility of our method, we also measured the survival curves of these M059K and M059J glioma cell lines by following the optimized protocol and obtained consistent results reported by others with cell sorting system. In conclusion, we successfully developed a reliable and simple way to measure the survival fractions of human cells exposed to low dose irradiation, which might be helpful for the studies on low-dose radiation biology.

• Research Organization: Brookhaven National Lab. (BNL), Upton, NY (United States)
• Sponsoring Organization: USDOE SC OFFICE OF BIOLOGICAL & ENVIRONMENTAL RESEARCH
• DOE Contract Number: DE-AC02-98CH10886
• OSTI ID: 1041622
• Report Number(s): BNL-96906-2012-JA; R&D Project: BO-086; KP1602020; TRN: US201212%%40
• Journal Information: International Research Journal of Biotechnology, Vol. 2, Issue 8; ISSN 2141-5153
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS
ANIMAL CELLS
BIOLOGY
CASKS
CELL CYCLE
EFFICIENCY
GLIOMAS
IRRADIATION
LOW DOSE IRRADIATION
PLATING
PROCESSING
RADIATIONS
RELIABILITY
SORTING
SURVIVAL CURVES
TRYPSIN
Colony forming assay
Low-density cell survival
Low-dose hyper-radiosensitivity