Solid-State Selective 13C Excitation and Spin Diffusion NMR to Resolve Spatial Dimensions in Plant Cell Walls
The average spatial dimensions between major biopolymers within the plant cell wall can be resolved using a solid-state NMR technique referred to as a {sup 13}C cross-polarization (CP) SELDOM (selectively by destruction of magnetization) with a mixing time delay for spin diffusion. Selective excitation of specific aromatic lignin carbons indicates that lignin is in close proximity to hemicellulose followed by amorphous and finally crystalline cellulose. {sup 13}C spin diffusion time constants (T{sub SD}) were extracted using a two-site spin diffusion theory developed for {sup 13}C nuclei under magic angle spinning (MAS) conditions. These time constants were then used to calculate an average lower-limit spin diffusion length between chemical groups within the plant cell wall. The results on untreated {sup 13}C enriched corn stover stem reveal that the lignin carbons are, on average, located at distances {approx}0.7-2.0 nm from the carbons in hemicellulose and cellulose, whereas the pretreated material had larger separations.
- Research Organization:
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Sponsoring Organization:
- USDOE Office of Science, BioEnergy Science Center
- DOE Contract Number:
- AC36-08GO28308
- OSTI ID:
- 1039091
- Report Number(s):
- NREL/JA-5100-54746; JAFCAU; TRN: US201209%%154
- Journal Information:
- Journal of Agricultural and Food Chemistry, Vol. 60, Issue 6; ISSN 0021-8561
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
AGRICULTURAL WASTES
AROMATICS
CELLULOSE
DIFFUSION
DIFFUSION LENGTH
DIMENSIONS
EXCITATION
HEMICELLULOSE
LIGNIN
MAGNETIZATION
MAIZE
NUCLEI
PLANT CELLS
SPIN
TIME DELAY
biomass
spin diffusion
plant cell wall
corn stover