Algal Biomass Constituent Analysis: Method Uncertainties and Investigation of the Underlying Measuring Chemistries
Algal biomass compositional analysis data form the basis of a large number of techno-economic process analysis models that are used to investigate and compare different processes in algal biofuels production. However, the analytical methods used to generate these data are far from standardized. This work investigated the applicability of common methods for rapid chemical analysis of biomass samples with respect to accuracy and precision. This study measured lipids, protein, carbohydrates, ash, and moisture of a single algal biomass sample at 3 institutions by 8 independent researchers over 12 separate workdays. Results show statistically significant differences in the results from a given analytical method among laboratories but not between analysts at individual laboratories, suggesting consistent training is a critical issue for empirical analytical methods. Significantly different results from multiple lipid and protein measurements were found to be due to different measurement chemistries. We identified a set of compositional analysis procedures that are in best agreement with data obtained by more advanced analytical procedures. The methods described here and used for the round robin experiment do not require specialized instrumentation, and with detailed analytical documentation, the differences between laboratories can be markedly reduced.
- Research Organization:
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Sponsoring Organization:
- USDOE Office of Energy Efficiency and Renewable Energy Biomass Program
- DOE Contract Number:
- AC36-08GO28308
- OSTI ID:
- 1038320
- Report Number(s):
- NREL/JA-5100-52719; TRN: US201208%%420
- Journal Information:
- Analytical Chemistry, Vol. 84, Issue 4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
ACCURACY
BIOFUELS
BIOMASS
CARBOHYDRATES
CHEMICAL ANALYSIS
DOCUMENT TYPES
DOCUMENTATION
LIPIDS
MOISTURE
PRODUCTION
PROTEINS
TRAINING
algae
algal biofuels
biofuels
production
processes
lipid based
oil extracts