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Title: Collagen fibril surface displays a constellation of sites capable of promoting fibril assembly, stability, and hemostasis

Abstract

Fibrillar collagens form the structural basis of organs and tissues including the vasculature, bone, and tendon. They are also dynamic, organizational scaffolds that present binding and recognition sites for ligands, cells, and platelets. We interpret recently published X-ray diffraction findings and use atomic force microscopy data to illustrate the significance of new insights into the functional organization of the collagen fibril. These data indicate that collagen's most crucial functional domains localize primarily to the overlap region, comprising a constellation of sites we call the 'master control region.' Moreover, the collagen's most exposed aspect contains its most stable part - the C-terminal region that controls collagen assembly, cross-linking, and blood clotting. Hidden beneath the fibril surface exists a constellation of 'cryptic' sequences poised to promote hemostasis and cell - collagen interactions in tissue injury and regeneration. These findings begin to address several important, and previously unresolved, questions: How functional domains are organized in the fibril, which domains are accessible, and which require proteolysis or structural trauma to become exposed? Here we speculate as to how collagen fibrillar organization impacts molecular processes relating to tissue growth, development, and repair.

Authors:
; ; ; ; ; ; ;  [1]
  1. IIT
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1031361
Resource Type:
Journal Article
Journal Name:
Connective Tissue Research
Additional Journal Information:
Journal Volume: 52; Journal Issue: 1
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; ADHESION; ATOMIC FORCE MICROSCOPY; BLOOD COAGULATION; COLLAGEN; FUNCTIONALS; INJURIES; ORGANS; PROTEOLYSIS; REGENERATION; REPAIR; STABILITY; X-RAY DIFFRACTION

Citation Formats

Orgel, J P, Antipova, O, Sagi, I, Bitler, A, Qiu, D, Wang, R, Xu, Y, and San Antonio, J D. Collagen fibril surface displays a constellation of sites capable of promoting fibril assembly, stability, and hemostasis. United States: N. p., 2011. Web. doi:10.3109/03008207.2010.511354.
Orgel, J P, Antipova, O, Sagi, I, Bitler, A, Qiu, D, Wang, R, Xu, Y, & San Antonio, J D. Collagen fibril surface displays a constellation of sites capable of promoting fibril assembly, stability, and hemostasis. United States. https://doi.org/10.3109/03008207.2010.511354
Orgel, J P, Antipova, O, Sagi, I, Bitler, A, Qiu, D, Wang, R, Xu, Y, and San Antonio, J D. 2011. "Collagen fibril surface displays a constellation of sites capable of promoting fibril assembly, stability, and hemostasis". United States. https://doi.org/10.3109/03008207.2010.511354.
@article{osti_1031361,
title = {Collagen fibril surface displays a constellation of sites capable of promoting fibril assembly, stability, and hemostasis},
author = {Orgel, J P and Antipova, O and Sagi, I and Bitler, A and Qiu, D and Wang, R and Xu, Y and San Antonio, J D},
abstractNote = {Fibrillar collagens form the structural basis of organs and tissues including the vasculature, bone, and tendon. They are also dynamic, organizational scaffolds that present binding and recognition sites for ligands, cells, and platelets. We interpret recently published X-ray diffraction findings and use atomic force microscopy data to illustrate the significance of new insights into the functional organization of the collagen fibril. These data indicate that collagen's most crucial functional domains localize primarily to the overlap region, comprising a constellation of sites we call the 'master control region.' Moreover, the collagen's most exposed aspect contains its most stable part - the C-terminal region that controls collagen assembly, cross-linking, and blood clotting. Hidden beneath the fibril surface exists a constellation of 'cryptic' sequences poised to promote hemostasis and cell - collagen interactions in tissue injury and regeneration. These findings begin to address several important, and previously unresolved, questions: How functional domains are organized in the fibril, which domains are accessible, and which require proteolysis or structural trauma to become exposed? Here we speculate as to how collagen fibrillar organization impacts molecular processes relating to tissue growth, development, and repair.},
doi = {10.3109/03008207.2010.511354},
url = {https://www.osti.gov/biblio/1031361}, journal = {Connective Tissue Research},
number = 1,
volume = 52,
place = {United States},
year = {Wed Dec 14 00:00:00 EST 2011},
month = {Wed Dec 14 00:00:00 EST 2011}
}