Microscale isoelectric fractionation using immobilized pH-specific membranes for multi-dimensional analysis.

Conference · Fri Oct 01 00:00:00 EDT 2010

OSTI ID:1030233

Mai, Junyu; Sommer, Gregory Jon; Hatch, Anson V

We report on advancements of our microscale isoelectric fractionation ({mu}IEFr) methodology for fast on-chip separation and concentration of proteins based on their isoelectric points (pI). We establish that proteins can be fractionated depending on posttranslational modifications into different pH specific bins, from where they can be efficiently transferred to downstream membranes for additional processing and analysis. This technology can enable on-chip multidimensional glycoproteomics analysis, as a new approach to expedite biomarker identification and verification.

• Research Organization: Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC04-94AL85000
• OSTI ID: 1030233
• Report Number(s): SAND2010-6972C; TRN: US201124%%31
• Resource Relation: Conference: Proposed for presentation at the 2010 microTAS Conference held October 5, 2010 in Groningen, The Netherlands.
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
FRACTIONATION
MEMBRANES
MODIFICATIONS
PROCESSING
PROTEINS
VERIFICATION