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Title: Glycerol-Induced Membrane Stiffening: The Role of Viscous Fluid Adlayers

Abstract

Lipid interfaces, ranging from cell membranes to thin surfactant layers that stabilize lung alveoli, are integral to living systems. Such interfaces are often subjected to mechanical forces, and because of their membrane-like geometry, they can easily deform by bending into localized folds. In this work, we explore the role of small molecules (i.e., glycerol) on the mechanical stability of model lung surfactant monolayers. We demonstrate that the presence of glycerol increases local monolayer bending stiffness by orders of magnitude. Our x-ray and neutron reflectivity measurements indicate that water is preferentially depleted, or glycerol is preferentially enriched, at the lipid headgroup/solvent interface, and that this glycerol-enriched layer extends O(10) beneath the monolayer with an adsorption free energy of 2.5 to 4.6 kJ/mol. The dramatic change in membrane bending stiffness in the presence of the sugar adlayer is understood in terms of two models: (1), lipid antiplasticization by glycerol; and (2), a continuum mechanical model of the viscous adlayer.

Authors:
; ; ; ; ; ; ; ;  [1]
  1. UNM
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
FOREIGNNSF
OSTI Identifier:
1024489
Resource Type:
Journal Article
Journal Name:
Biophys. J.
Additional Journal Information:
Journal Volume: 101; Journal Issue: (1) ; 07, 2011; Journal ID: ISSN 0006-3495
Country of Publication:
United States
Language:
ENGLISH
Subject:
72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS; ADSORPTION; BENDING; CELL MEMBRANES; FLEXIBILITY; FREE ENERGY; GEOMETRY; GLYCEROL; LIPIDS; LUNGS; MEMBRANES; NEUTRONS; REFLECTIVITY; SACCHAROSE; STABILITY; SURFACTANTS; WATER

Citation Formats

Pocivavsek, Luka, Gavrilov, Kseniya, Cao, Kathleen D, Chi, Eva Y, Li, Dongxu, Lin, Binhua, Meron, Mati, Majewski, Jaroslaw, Lee, Ka Yee C, UC), and LANL). Glycerol-Induced Membrane Stiffening: The Role of Viscous Fluid Adlayers. United States: N. p., 2011. Web. doi:10.1016/j.bpj.2011.05.036.
Pocivavsek, Luka, Gavrilov, Kseniya, Cao, Kathleen D, Chi, Eva Y, Li, Dongxu, Lin, Binhua, Meron, Mati, Majewski, Jaroslaw, Lee, Ka Yee C, UC), & LANL). Glycerol-Induced Membrane Stiffening: The Role of Viscous Fluid Adlayers. United States. https://doi.org/10.1016/j.bpj.2011.05.036
Pocivavsek, Luka, Gavrilov, Kseniya, Cao, Kathleen D, Chi, Eva Y, Li, Dongxu, Lin, Binhua, Meron, Mati, Majewski, Jaroslaw, Lee, Ka Yee C, UC), and LANL). 2011. "Glycerol-Induced Membrane Stiffening: The Role of Viscous Fluid Adlayers". United States. https://doi.org/10.1016/j.bpj.2011.05.036.
@article{osti_1024489,
title = {Glycerol-Induced Membrane Stiffening: The Role of Viscous Fluid Adlayers},
author = {Pocivavsek, Luka and Gavrilov, Kseniya and Cao, Kathleen D and Chi, Eva Y and Li, Dongxu and Lin, Binhua and Meron, Mati and Majewski, Jaroslaw and Lee, Ka Yee C and UC) and LANL)},
abstractNote = {Lipid interfaces, ranging from cell membranes to thin surfactant layers that stabilize lung alveoli, are integral to living systems. Such interfaces are often subjected to mechanical forces, and because of their membrane-like geometry, they can easily deform by bending into localized folds. In this work, we explore the role of small molecules (i.e., glycerol) on the mechanical stability of model lung surfactant monolayers. We demonstrate that the presence of glycerol increases local monolayer bending stiffness by orders of magnitude. Our x-ray and neutron reflectivity measurements indicate that water is preferentially depleted, or glycerol is preferentially enriched, at the lipid headgroup/solvent interface, and that this glycerol-enriched layer extends O(10) beneath the monolayer with an adsorption free energy of 2.5 to 4.6 kJ/mol. The dramatic change in membrane bending stiffness in the presence of the sugar adlayer is understood in terms of two models: (1), lipid antiplasticization by glycerol; and (2), a continuum mechanical model of the viscous adlayer.},
doi = {10.1016/j.bpj.2011.05.036},
url = {https://www.osti.gov/biblio/1024489}, journal = {Biophys. J.},
issn = {0006-3495},
number = (1) ; 07, 2011,
volume = 101,
place = {United States},
year = {Fri Dec 09 00:00:00 EST 2011},
month = {Fri Dec 09 00:00:00 EST 2011}
}