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Title: Responses of soil cellulolytic fungal communities to elevated atmospheric CO2 are complex and variableacross five ecosystems

Abstract

Elevated atmospheric CO(2) generally increases plant productivity and subsequently increases the availability of cellulose in soil to microbial decomposers. As key cellulose degraders, soil fungi are likely to be one of the most impacted and responsive microbial groups to elevated atmospheric CO(2) . To investigate the impacts of ecosystem type and elevated atmospheric CO(2) on cellulolytic fungal communities, we sequenced 10 677 cbhI gene fragments encoding the catalytic subunit of cellobiohydrolase I, across five distinct terrestrial ecosystem experiments after a decade of exposure to elevated CO(2) . The cbhI composition of each ecosystem was distinct, as supported by weighted Unifrac analyses (all P-values; < 0.001), with few operational taxonomic units (OTUs) being shared across ecosystems. Using a 114-member cbhI sequence database compiled from known fungi, less than 1% of the environmental sequences could be classified at the family level indicating that cellulolytic fungi in situ are likely dominated by novel fungi or known fungi that are not yet recognized as cellulose degraders. Shifts in fungal cbhI composition and richness that were correlated with elevated CO(2) exposure varied across the ecosystems. In aspen plantation and desert creosote bush soils, cbhI gene richness was significantly higher after exposure to elevated CO(2) (550more » mol mol(-1) ) than under ambient CO(2) (360 mol mol(-1) CO(2) ). In contrast, while the richness was not altered, the relative abundance of dominant OTUs in desert soil crusts was significantly shifted. This suggests that responses are complex, vary across different ecosystems and, in at least one case, are OTU-specific. Collectively, our results document the complexity of cellulolytic fungal communities in multiple terrestrial ecosystems and the variability of their responses to long-term exposure to elevated atmospheric CO(2) .« less

Authors:
 [1];  [2];  [3];  [4];  [4];  [4];  [5];  [6];  [1]
  1. Los Alamos National Laboratory (LANL)
  2. University of Michigan
  3. Northern Arizona University
  4. Duke University
  5. ORNL
  6. Smithsonian
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1024292
DOE Contract Number:  
DE-AC05-00OR22725
Resource Type:
Journal Article
Journal Name:
Environmental Microbiology
Additional Journal Information:
Journal Volume: 13; Journal Issue: 10; Journal ID: ISSN 1462-2912
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; ABUNDANCE; ASPENS; AVAILABILITY; BIODEGRADATION; CARBON DIOXIDE; CELLULOSE; DECOMPOSITION; CREOSOTE; DESERTS; ECOSYSTEMS; FUNGI; GENES; PRODUCTIVITY; SOILS; TERRESTRIAL ECOSYSTEMS; soil; cellulolytic fungal communities; variableacross five ecosystems

Citation Formats

Weber, Carolyn F, Zak, Donald R, Hungate, Bruce, Jackson, Robert B, Vilgalys, Rytas, Evans, R David, Schadt, Christopher Warren, Megonigal, J. Patrick, and Kuske, Cheryl R. Responses of soil cellulolytic fungal communities to elevated atmospheric CO2 are complex and variableacross five ecosystems. United States: N. p., 2011. Web. doi:10.1111/j.1462-2920.2011.02548.x.
Weber, Carolyn F, Zak, Donald R, Hungate, Bruce, Jackson, Robert B, Vilgalys, Rytas, Evans, R David, Schadt, Christopher Warren, Megonigal, J. Patrick, & Kuske, Cheryl R. Responses of soil cellulolytic fungal communities to elevated atmospheric CO2 are complex and variableacross five ecosystems. United States. https://doi.org/10.1111/j.1462-2920.2011.02548.x
Weber, Carolyn F, Zak, Donald R, Hungate, Bruce, Jackson, Robert B, Vilgalys, Rytas, Evans, R David, Schadt, Christopher Warren, Megonigal, J. Patrick, and Kuske, Cheryl R. 2011. "Responses of soil cellulolytic fungal communities to elevated atmospheric CO2 are complex and variableacross five ecosystems". United States. https://doi.org/10.1111/j.1462-2920.2011.02548.x.
@article{osti_1024292,
title = {Responses of soil cellulolytic fungal communities to elevated atmospheric CO2 are complex and variableacross five ecosystems},
author = {Weber, Carolyn F and Zak, Donald R and Hungate, Bruce and Jackson, Robert B and Vilgalys, Rytas and Evans, R David and Schadt, Christopher Warren and Megonigal, J. Patrick and Kuske, Cheryl R},
abstractNote = {Elevated atmospheric CO(2) generally increases plant productivity and subsequently increases the availability of cellulose in soil to microbial decomposers. As key cellulose degraders, soil fungi are likely to be one of the most impacted and responsive microbial groups to elevated atmospheric CO(2) . To investigate the impacts of ecosystem type and elevated atmospheric CO(2) on cellulolytic fungal communities, we sequenced 10 677 cbhI gene fragments encoding the catalytic subunit of cellobiohydrolase I, across five distinct terrestrial ecosystem experiments after a decade of exposure to elevated CO(2) . The cbhI composition of each ecosystem was distinct, as supported by weighted Unifrac analyses (all P-values; < 0.001), with few operational taxonomic units (OTUs) being shared across ecosystems. Using a 114-member cbhI sequence database compiled from known fungi, less than 1% of the environmental sequences could be classified at the family level indicating that cellulolytic fungi in situ are likely dominated by novel fungi or known fungi that are not yet recognized as cellulose degraders. Shifts in fungal cbhI composition and richness that were correlated with elevated CO(2) exposure varied across the ecosystems. In aspen plantation and desert creosote bush soils, cbhI gene richness was significantly higher after exposure to elevated CO(2) (550 mol mol(-1) ) than under ambient CO(2) (360 mol mol(-1) CO(2) ). In contrast, while the richness was not altered, the relative abundance of dominant OTUs in desert soil crusts was significantly shifted. This suggests that responses are complex, vary across different ecosystems and, in at least one case, are OTU-specific. Collectively, our results document the complexity of cellulolytic fungal communities in multiple terrestrial ecosystems and the variability of their responses to long-term exposure to elevated atmospheric CO(2) .},
doi = {10.1111/j.1462-2920.2011.02548.x},
url = {https://www.osti.gov/biblio/1024292}, journal = {Environmental Microbiology},
issn = {1462-2912},
number = 10,
volume = 13,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 2011},
month = {Sat Jan 01 00:00:00 EST 2011}
}