Digital microfluidic hub for automated nucleic acid sample preparation.
Abstract
We have designed, fabricated, and characterized a digital microfluidic (DMF) platform to function as a central hub for interfacing multiple lab-on-a-chip sample processing modules towards automating the preparation of clinically-derived DNA samples for ultrahigh throughput sequencing (UHTS). The platform enables plug-and-play installation of a two-plate DMF device with consistent spacing, offers flexible connectivity for transferring samples between modules, and uses an intuitive programmable interface to control droplet/electrode actuations. Additionally, the hub platform uses transparent indium-tin oxide (ITO) electrodes to allow complete top and bottom optical access to the droplets on the DMF array, providing additional flexibility for various detection schemes.
- Authors:
- Publication Date:
- Research Org.:
- Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1021631
- Report Number(s):
- SAND2010-4960C
TRN: US201117%%225
- DOE Contract Number:
- AC04-94AL85000
- Resource Type:
- Conference
- Resource Relation:
- Conference: Proposed for presentation at the MicroTAS 2010 Conference held October 3-7, 2010 in Groningen, Netherlands.
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE; DETECTION; DNA; DNA SEQUENCING; ELECTRODES; FLEXIBILITY; NUCLEIC ACIDS; OXIDES; PROCESSING; SAMPLE PREPARATION
Citation Formats
He, Jim, Bartsch, Michael S, Patel, Kamlesh D, Kittlaus, Eric A, Remillared, Erin M, Pezzola, Genevieve L, Renzi, Ronald F, and Kim, Hanyoup. Digital microfluidic hub for automated nucleic acid sample preparation.. United States: N. p., 2010.
Web.
He, Jim, Bartsch, Michael S, Patel, Kamlesh D, Kittlaus, Eric A, Remillared, Erin M, Pezzola, Genevieve L, Renzi, Ronald F, & Kim, Hanyoup. Digital microfluidic hub for automated nucleic acid sample preparation.. United States.
He, Jim, Bartsch, Michael S, Patel, Kamlesh D, Kittlaus, Eric A, Remillared, Erin M, Pezzola, Genevieve L, Renzi, Ronald F, and Kim, Hanyoup. 2010.
"Digital microfluidic hub for automated nucleic acid sample preparation.". United States.
@article{osti_1021631,
title = {Digital microfluidic hub for automated nucleic acid sample preparation.},
author = {He, Jim and Bartsch, Michael S and Patel, Kamlesh D and Kittlaus, Eric A and Remillared, Erin M and Pezzola, Genevieve L and Renzi, Ronald F and Kim, Hanyoup},
abstractNote = {We have designed, fabricated, and characterized a digital microfluidic (DMF) platform to function as a central hub for interfacing multiple lab-on-a-chip sample processing modules towards automating the preparation of clinically-derived DNA samples for ultrahigh throughput sequencing (UHTS). The platform enables plug-and-play installation of a two-plate DMF device with consistent spacing, offers flexible connectivity for transferring samples between modules, and uses an intuitive programmable interface to control droplet/electrode actuations. Additionally, the hub platform uses transparent indium-tin oxide (ITO) electrodes to allow complete top and bottom optical access to the droplets on the DMF array, providing additional flexibility for various detection schemes.},
doi = {},
url = {https://www.osti.gov/biblio/1021631},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Jul 01 00:00:00 EDT 2010},
month = {Thu Jul 01 00:00:00 EDT 2010}
}