Concomitant Microbial Generation of Palladium Nanoparticles and Hydrogen To Immobilize Chromate

Chidambaram, D; Hennebel, T; Taghavi, S; Mast, J; Boon, N; Verstraete, W; Van Der Lelie, D; Fitts, J

doi:10.1021/es101559r

Title: Concomitant Microbial Generation of Palladium Nanoparticles and Hydrogen To Immobilize Chromate

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Abstract

The catalytic properties of various metal nanoparticles have led to their use in environmental remediation. Our aim is to develop and apply an efficient bioremediation method based on in situ biosynthesis of bio-Pd nanoparticles and hydrogen. C. pasteurianum BC1 was used to reduce Pd(II) ions to form Pd nanoparticles (bio-Pd) that primarily precipitated on the cell wall and in the cytoplasm. C. pasteurianum BC1 cells, loaded with bio-Pd nanoparticle in the presence of glucose, were subsequently used to fermentatively produce hydrogen and to effectively catalyze the removal of soluble Cr(VI) via reductive transformation to insoluble Cr(III) species. Batch and aquifer microcosm experiments using C. pasteurianum BC1 cells loaded with bio-Pd showed efficient reductive Cr(VI) removal, while in control experiments with killed or viable but Pd-free bacterial cultures no reductive Cr(VI) removal was observed. Our results suggest a novel process where the in situ microbial production of hydrogen is directly coupled to the catalytic bio-Pd mediated reduction of chromate. This process offers significant advantages over the current groundwater treatment technologies that rely on introducing preformed catalytic nanoparticles into groundwater treatment zones and the costly addition of molecular hydrogen to above ground pump and treat systems.

Authors:: Chidambaram, D; Hennebel, T; Taghavi, S; Mast, J; Boon, N; Verstraete, W; Van Der Lelie, D; Fitts, J

Publication Date:: Fri Jan 01 00:00:00 EST 2010

Research Org.:: Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source

Sponsoring Org.:: DOE - OFFICE OF SCIENCE

OSTI Identifier:: 1019842

Report Number(s):: BNL-95688-2011-JA
Journal ID: ISSN 0013-936X; ESTHAG; TRN: US201115%%478

DOE Contract Number:: DE-AC02-98CH10886

Resource Type:: Journal Article

Journal Name:: Environmental Science and Technology

Additional Journal Information:: Journal Volume: 44; Journal Issue: 19; Journal ID: ISSN 0013-936X

Country of Publication:: United States

Language:: English

Subject:: 08 HYDROGEN; AQUIFERS; BIOREMEDIATION; BIOSYNTHESIS; CELL WALL; CHROMATES; CYTOPLASM; GLUCOSE; HYDROGEN; MICROCOSMS; PALLADIUM; PRODUCTION; REMOVAL; TRANSFORMATIONS; national synchrotron light source

Citation Formats


                    Chidambaram, D, Hennebel, T, Taghavi, S, Mast, J, Boon, N, Verstraete, W, Van Der Lelie, D, and Fitts, J. Concomitant Microbial Generation of Palladium Nanoparticles and Hydrogen To Immobilize Chromate.  United States: N. p., 2010. 
        Web.  doi:10.1021/es101559r.

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                    Chidambaram, D, Hennebel, T, Taghavi, S, Mast, J, Boon, N, Verstraete, W, Van Der Lelie, D, & Fitts, J. Concomitant Microbial Generation of Palladium Nanoparticles and Hydrogen To Immobilize Chromate.  United States.  https://doi.org/10.1021/es101559r

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                    Chidambaram, D, Hennebel, T, Taghavi, S, Mast, J, Boon, N, Verstraete, W, Van Der Lelie, D, and Fitts, J. 2010.  
        "Concomitant Microbial Generation of Palladium Nanoparticles and Hydrogen To Immobilize Chromate".  United States.  https://doi.org/10.1021/es101559r.

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@article{osti_1019842,

  title        = {Concomitant Microbial Generation of Palladium Nanoparticles and Hydrogen To Immobilize Chromate},

  author       = {Chidambaram, D and Hennebel, T and Taghavi, S and Mast, J and Boon, N and Verstraete, W and Van Der Lelie, D and Fitts, J},

  abstractNote = {The catalytic properties of various metal nanoparticles have led to their use in environmental remediation. Our aim is to develop and apply an efficient bioremediation method based on in situ biosynthesis of bio-Pd nanoparticles and hydrogen. C. pasteurianum BC1 was used to reduce Pd(II) ions to form Pd nanoparticles (bio-Pd) that primarily precipitated on the cell wall and in the cytoplasm. C. pasteurianum BC1 cells, loaded with bio-Pd nanoparticle in the presence of glucose, were subsequently used to fermentatively produce hydrogen and to effectively catalyze the removal of soluble Cr(VI) via reductive transformation to insoluble Cr(III) species. Batch and aquifer microcosm experiments using C. pasteurianum BC1 cells loaded with bio-Pd showed efficient reductive Cr(VI) removal, while in control experiments with killed or viable but Pd-free bacterial cultures no reductive Cr(VI) removal was observed. Our results suggest a novel process where the in situ microbial production of hydrogen is directly coupled to the catalytic bio-Pd mediated reduction of chromate. This process offers significant advantages over the current groundwater treatment technologies that rely on introducing preformed catalytic nanoparticles into groundwater treatment zones and the costly addition of molecular hydrogen to above ground pump and treat systems.},

  doi          = {10.1021/es101559r},

  url          = {https://www.osti.gov/biblio/1019842},
  journal      = {Environmental Science and Technology},

  issn         = {0013-936X},

  number       = 19,

  volume       = 44,

  place        = {United States},

  year         = {Fri Jan 01 00:00:00 EST 2010},

  month        = {Fri Jan 01 00:00:00 EST 2010}

}

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