Expression, purification, crystallization and preliminary X-ray analysis of Aeromonas hydrophilia metallo-[beta]-lactamase
Journal Article
·
· Acta Crystallogr. F
OSTI ID:1008485
- Merck
The CphA metallo-{beta}-lactamase from Aeromonas hydrophilia has been expressed, purified and crystallized by the hanging-drop vapor-diffusion method using ammonium sulfate as the precipitant. The crystals exhibit orthorhombic symmetry (P2{sub 1}2{sub 1}2), with unit-cell parameters a = 40.75, b = 42.05, c = 128.88 {angstrom}. There is one monomer in the asymmetric unit and the solvent content is estimated to be 44% by volume. A data set extending to 1.8 {angstrom} has been measured.
- Research Organization:
- Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 1008485
- Journal Information:
- Acta Crystallogr. F, Vol. 61, Issue (2) ; 2005; ISSN 1744-3091
- Country of Publication:
- United States
- Language:
- ENGLISH
Similar Records
Expression, purification, crystallization and preliminary X-ray analysis of Aeromonas hydrophilia metallo-β-lactamase
The metallo-. beta. -lactamases of Bacillus cereus 5/B/6: Expression in Echierichia coli, purification, and characterization of the purified recombinant enzyme
Expression, purification, crystallization, and preliminary X-ray crystallographic analysis of OXA-17, an extended-spectrum {beta}-lactamase conferring severe antibiotic resistance
Journal Article
·
Tue Feb 01 00:00:00 EST 2005
· Acta Crystallographica. Section F
·
OSTI ID:1008485
The metallo-. beta. -lactamases of Bacillus cereus 5/B/6: Expression in Echierichia coli, purification, and characterization of the purified recombinant enzyme
Conference
·
Mon Mar 11 00:00:00 EST 1991
· FASEB Journal (Federation of American Societies for Experimental Biology); (United States)
·
OSTI ID:1008485
+1 more
Expression, purification, crystallization, and preliminary X-ray crystallographic analysis of OXA-17, an extended-spectrum {beta}-lactamase conferring severe antibiotic resistance
Journal Article
·
Mon Jul 15 00:00:00 EDT 2013
· Crystallography Reports
·
OSTI ID:1008485