Genome Sequence of the Obligate Methanotroph Methylosinus trichosporium Strain OB3b
- University of Alberta, Edmondton, Canada
- University of Michigan
- University of Iowa
- University of Warwick, UK
- Defence Science and Detection Department (Dstl), Wiltshire, UK
- University of Strasbourg
- University of Washington, Seattle
- Los Alamos National Laboratory (LANL)
- U.S. Department of Energy, Joint Genome Institute
- ORNL
- Radboud University Nijmegen, The Netherlands
- Genoscope/Centre National de la Recherche Scientifique-Unite Mixte de Recherche
- University of Louisville, Louisville
Methylosinus trichosporium OB3b (for "oddball" strain 3b) is an obligate aerobic methane-oxidizing alphaproteobacterium that was originally isolated in 1970 by Roger Whittenbury and colleagues. This strain has since been used extensively to elucidate the structure and function of several key enzymes of methane oxidation, including both particulate and soluble methane monooxygenase (sMMO) and the extracellular copper chelator methanobactin. In particular, the catalytic properties of soluble methane monooxygenase from M. trichosporium OB3b have been well characterized in context with biodegradation of recalcitrant hydrocarbons, such as trichloroethylene. The sequence of the M. trichosporium OB3b genome is the first reported from a member of the Methylocystaceae family in the order Rhizobiales.
- Research Organization:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Sponsoring Organization:
- Work for Others (WFO)
- DOE Contract Number:
- DE-AC05-00OR22725
- OSTI ID:
- 1007842
- Journal Information:
- Journal of Bacteriology, Vol. 192, Issue 24; ISSN 0021--9193
- Country of Publication:
- United States
- Language:
- English
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