Structural Basis of UV DNA-Damage Recognition by the DDB1-DDB2 Complex

Journal Article · Thu Apr 02 00:00:00 EDT 2009 · Cell

DOI:https://doi.org/10.1016/j.cell.2008.10.045· OSTI ID:1007062

Scrima, Andrea; ková, Renata; Czyzewski, Bryan K; Kawasaki, Yusuke; Jeffrey, Philip D; Groisman, Regina; Nakatani, Yoshihiro; Iwai, Shigenori; Pavletich, Nikola P; Thomä, Nicolas H ^[1]

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1. CNRS-CRMD

Ultraviolet (UV) light-induced pyrimidine photodimers are repaired by the nucleotide excision repair pathway. Photolesions have biophysical parameters closely resembling undamaged DNA, impeding discovery through damage surveillance proteins. The DDB1DDB2 complex serves in the initial detection of UV lesions in vivo. Here we present the structures of the DDB1DDB2 complex alone and bound to DNA containing either a 6-4 pyrimidine-pyrimidone photodimer (6-4PP) lesion or an abasic site. The structure shows that the lesion is held exclusively by the WD40 domain of DDB2. A DDB2 hairpin inserts into the minor groove, extrudes the photodimer into a binding pocket, and kinks the duplex by {approx}40*. The tightly localized probing of the photolesions, combined with proofreading in the photodimer pocket, enables DDB2 to detect lesions refractory to detection by other damage surveillance proteins. The structure provides insights into damage recognition in chromatin and suggests a mechanism by which the DDB1-associated CUL4 ubiquitin ligase targets proteins surrounding the site of damage.

• Research Organization: Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
• Sponsoring Organization: USDOE
• OSTI ID: 1007062
• Journal Information: Cell, Vol. 135, Issue (7) ; 12, 2008; ISSN 0092-8674
• Country of Publication: United States
• Language: ENGLISH

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
CHROMATIN
DETECTION
DNA
EXCISION REPAIR
IN VIVO
LIGASES
NUCLEOTIDES
PROTEINS
PYRIMIDINES
TARGETS