Microbial Diversity and Bioremediation of a Hydrocarbon-Contaminated Aquifer (Vega Baja, Puerto Rico)

Rodriguez-Martinex, Enid M.; Perez, Ernie; Schadt, Christopher Warren; Zhou, Jizhong; Massol-Deya, Arturo A.

doi:10.3390/ijerph2006030036

Title: Microbial Diversity and Bioremediation of a Hydrocarbon-Contaminated Aquifer (Vega Baja, Puerto Rico)

Full Record
Other Related Research

Abstract

Hydrocarbon contamination of groundwater resources has become a major environmental and human health concern in many parts of the world. Our objectives were to employ both culture and culture-independent techniques to characterize the dynamics of microbial community structure within a fluidized bed reactor used to bioremediate a diesel-contaminated groundwater in a tropical environment. Under normal operating conditions, 97 to 99% of total hydrocarbons were removed with only 14 min hydraulic retention time. Over 25 different cultures were isolated from the treatment unit (96% which utilized diesel constituents as sole carbon source). Approximately 20% of the isolates were also capable of complete denitrification to nitrogen gas. Sequence analysis of 16S rDNA demonstrated ample diversity with most belonging to the {infinity}, {beta} and {gamma} subdivision of the Proteobacteria, Bacilli, and Actinobacteria groups. Moreover, the genetic constitution of the microbial community was examined at multiple time points with a Functional Gene Array (FGA) containing over 12,000 probes for genes involved in organic degradation and major biogeochemical cycles. Total community DNA was extracted and amplified using an isothermal {phi}29 polymerase-based technique, labeled with Cy5 dye, and hybridized to the arrays in 50% formimide overnight at 50 C. Cluster analysis revealed comparable profiles over themore »« less

Authors:

Rodriguez-Martinex, Enid M. ^[1]; Perez, Ernie ^[1]; Schadt, Christopher Warren ^[2]; Zhou, Jizhong ^[3]; Massol-Deya, Arturo A. ^[1]

University of Puerto Rico
ORNL
University of Oklahoma

Publication Date:: Sun Jan 01 00:00:00 EST 2006

Research Org.:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:: USDOE Office of Science (SC)

OSTI Identifier:: 1003692

DOE Contract Number:: DE-AC05-00OR22725

Resource Type:: Journal Article

Journal Name:: International Journal of Environmental Research and Public Health

Additional Journal Information:: Journal Volume: 3; Journal Issue: 3; Journal ID: ISSN 1660--4601

Country of Publication:: United States

Language:: English

Subject:: 03 NATURAL GAS; AQUIFERS; BIOREMEDIATION; CONTAMINATION; CYTOCHROMES; DENITRIFICATION; DNA; FLUIDIZED BED REACTORS; GENES; GENETICS; GROUND WATER; HYDROCARBONS; METHANE; NITROGEN; NITROUS OXIDE; OXIDOREDUCTASES; STRUCTURAL CHEMICAL ANALYSIS; SULFITES; Microarray; Groundwater; Contaminant Degradation

Citation Formats


                    Rodriguez-Martinex, Enid M., Perez, Ernie, Schadt, Christopher Warren, Zhou, Jizhong, and Massol-Deya, Arturo A. Microbial Diversity and Bioremediation of a Hydrocarbon-Contaminated Aquifer (Vega Baja, Puerto Rico).  United States: N. p., 2006. 
        Web.  doi:10.3390/ijerph2006030036.

Copy to clipboard


                    Rodriguez-Martinex, Enid M., Perez, Ernie, Schadt, Christopher Warren, Zhou, Jizhong, & Massol-Deya, Arturo A. Microbial Diversity and Bioremediation of a Hydrocarbon-Contaminated Aquifer (Vega Baja, Puerto Rico).  United States.  https://doi.org/10.3390/ijerph2006030036

Copy to clipboard


                    Rodriguez-Martinex, Enid M., Perez, Ernie, Schadt, Christopher Warren, Zhou, Jizhong, and Massol-Deya, Arturo A. 2006.  
        "Microbial Diversity and Bioremediation of a Hydrocarbon-Contaminated Aquifer (Vega Baja, Puerto Rico)".  United States.  https://doi.org/10.3390/ijerph2006030036.

Copy to clipboard


                    
@article{osti_1003692,

  title        = {Microbial Diversity and Bioremediation of a Hydrocarbon-Contaminated Aquifer (Vega Baja, Puerto Rico)},

  author       = {Rodriguez-Martinex, Enid M. and Perez, Ernie and Schadt, Christopher Warren and Zhou, Jizhong and Massol-Deya, Arturo A.},

  abstractNote = {Hydrocarbon contamination of groundwater resources has become a major environmental and human health concern in many parts of the world. Our objectives were to employ both culture and culture-independent techniques to characterize the dynamics of microbial community structure within a fluidized bed reactor used to bioremediate a diesel-contaminated groundwater in a tropical environment. Under normal operating conditions, 97 to 99% of total hydrocarbons were removed with only 14 min hydraulic retention time. Over 25 different cultures were isolated from the treatment unit (96% which utilized diesel constituents as sole carbon source). Approximately 20% of the isolates were also capable of complete denitrification to nitrogen gas. Sequence analysis of 16S rDNA demonstrated ample diversity with most belonging to the {infinity}, {beta} and {gamma} subdivision of the Proteobacteria, Bacilli, and Actinobacteria groups. Moreover, the genetic constitution of the microbial community was examined at multiple time points with a Functional Gene Array (FGA) containing over 12,000 probes for genes involved in organic degradation and major biogeochemical cycles. Total community DNA was extracted and amplified using an isothermal {phi}29 polymerase-based technique, labeled with Cy5 dye, and hybridized to the arrays in 50% formimide overnight at 50 C. Cluster analysis revealed comparable profiles over the course of treatment suggesting the early selection of a very stable microbial community. A total of 270 genes for organic contaminant degradation (including naphthalene, toluene [aerobic and anaerobic], octane, biphenyl, pyrene, xylene, phenanthrene, and benzene); and 333 genes involved in metabolic activities (nitrite and nitrous oxide reductases [nirS, nirK, and nosZ], dissimilatory sulfite reductases [dsrAB], potential metal reducing C-type cytochromes, and methane monooxygenase [pmoA]) were repeatedly detected. Genes for degradation of MTBE, nitroaromatics and chlorinated compounds were also present, indicating a broad catabolic potential of the treatment unit. FGA's demonstrated the early establishment of a diverse community with concurrent aerobic and anaerobic processes contributing to the bioremediation process.},

  doi          = {10.3390/ijerph2006030036},

  url          = {https://www.osti.gov/biblio/1003692},
  journal      = {International Journal of Environmental Research and Public Health},

  issn         = {1660--4601},

  number       = 3,

  volume       = 3,

  place        = {United States},

  year         = {Sun Jan 01 00:00:00 EST 2006},

  month        = {Sun Jan 01 00:00:00 EST 2006}

}

Copy to clipboard

Journal Article:

https://doi.org/10.3390/ijerph2006030036

Other availability

Find in Google Scholar

Search WorldCat to find libraries that may hold this journal

Save / Share:

Export Metadata

Save to My Library

Similar records in OSTI.GOV collections:

Microbial Diversity and Bioremediation of aHydrocarbon-Contaminated Aquifer (Vega Baja, Puerto Rico)

Journal Article Rodriguez-Martinez, E; Perez, Ernie; Schadt, ChristopherW; ... - International Journal of Environmental Research and PublicHealth

Hydrocarbon contamination of groundwater resources hasbecome a major environmental and human health concern in many parts ofthe world. Our objectives were to employ both culture andculture-independent techniques to characterize the dynamics of microbialcommunity structure within a fluidized bed reactor used to bioremediate adiesel-contaminated groundwater in a tropical environment. Under normaloperating conditions, 97 to 99 percent of total hydrocarbons were removedwith only 14 min hydraulic retention time. Over 25 different cultureswere isolated from the treatment unit (96 percent which utilized dieselconstituents as sole carbon source). Approximately 20 percent of theisolates were also capable of complete denitrification to nitrogen gas.Sequence analysis ofmore »« less
Comparison of multiple ecogenomics methods for determiningecosystem function in uranium-contaminated environments

Conference Hazen, T; Dehal, P; Arkin, A; ...

Background: Bioremediation may offer the only feasiblestrategy for the nearly intractable problem of metal and radionuclidecontamination of soil and groundwater. To understand bioremediation incontaminated environments, it is critical to determine the organismspresent in these environments, analyze their responses to stressconditions, and elucidate functional position in the environment.Methods: We used multiple molecular techniques on both sediment andgroundwater to develop a better understanding of the functionalcapability and stress level within the microbial community inrelationship to over one hundred geochemical parameters. Due to the lowpH (3.5-4.5) and high contaminant levels (e.g., uranium) microbialdensities and activities were low. We used a phage polymeraseamplification systemmore »« less
Full Text Available
Phylogenetic and Functional Biomakers as Indicators of Bacterial Community Responses to Mixed-Waste Contamination

Journal Article Fields, Matthew; Carroll, Sue; Watson, David; ... - Environmental Science & Technology

Few studies have demonstrated changes in community structure along a contaminant plume in terms of phylogenetic, functional, and geochemical changes, and such studies are essential to understand how a microbial ecosystem responds to perturbations. Clonal libraries of multiple genes (SSU rDNA, nirK, nirS, amoA, pmoA, and dsrAB) were analyzed from groundwater samples (n = 6) that varied in contaminant levels, and 107 geochemical parameters were measured. Principal components analyses (PCA) were used to compare the relationships among the sites with respect to the biomarker (n = 785 for all sequences) distributions and the geochemical variables. A major portion of themore »« less
https://doi.org/10.1021/es051748q
Microbial Community Functional Structures in Wastewater Treatment Plants as Characterized by GeoChip

Journal Article Wang, Xiaohui; Xia, Yu; Wen, Xianghua; ... - PLoS ONE

Background: Biological WWTPs must be functionally stable to continuously and steadily remove contaminants which rely upon the activity of complex microbial communities. However, knowledge is still lacking in regard to microbial community functional structures and their linkages to environmental variables. Aims: To investigate microbial community functional structures of activated sludge in wastewater treatment plants (WWTPs) and to understand the effects of environmental factors on their structure. Methods: 12 activated sludge samples were collected from four WWTPs in Beijing. A comprehensive functional gene array named GeoChip 4.2 was used to determine the microbial functional genes involved in a variety of biogeochemicalmore »« less
https://doi.org/10.1371/journal.pone.0093422

Full Text Available
Subsidized or stressed? Shifts in freshwater benthic microbial metagenomics along a gradient of alkaline coal mine drainage

Journal Article Bier, Raven; Wernegreen, Jennifer J.; Vilgalys, Rytas; ... - Limnology and Oceanography

Chemical pollution mixtures enter aquatic environments and interact with microorganisms in eclectic ways with disparate consequences for microbial ecosystem services. Can using a thermodynamic framework help to determine the net influence of a chemical mixture on the functional capacity of benthic microbial communities? In this study, we examined this question by comparing benthic stream microbial communities exposed to a gradient of neutral-alkaline coalmine effluent. Using a combination of approaches (metagenomics, quantitative polymerase chain reaction [qPCR], and functional assays), we show that functional genes and pathways of microbial communities growing in mine effluent differed in composition, but not diversity. The majoritymore »« less
Cited by 5
https://doi.org/10.1002/lno.11301

Full Text Available

Similar Records