Analysis of Biostimulated Microbial Communities from Two Field Experiments Reveals Temporal and Spatial Differences in Proteome Profiles

Journal Article · Fri Jan 01 00:00:00 EST 2010 · Environmental Science & Technology

DOI:https://doi.org/10.1021/es101029f· OSTI ID:1000727

Callister, Stephen J ^[1]; Wilkins, Mike ^[2]; Nicora, Carrie D. ^[1]; Williams, Ken ^[3]; Banfield, Jillian F. ^[2]; Verberkmoes, Nathan C ^[4]; N'Guessan, A. Lucie ^[5]; Mouser, Paula J ^[5]; Elifantz, Hila ^[5]; Smith, Richard D. ^[1]; Lovley, Derek ^[5]; Lipton, Mary S ^[1]; Long, Phil ^[1]

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1. Pacific Northwest National Laboratory (PNNL)
2. University of California, Berkeley
3. Lawrence Berkeley National Laboratory (LBNL)
4. ORNL
5. University of Massachusetts, Amherst

Stimulated by an acetate-amendment field experiment conducted in 2007, anaerobic microbial populations in the aquifer at the Rifle Integrated Field Research Challenge site in Colorado reduced mobile U(VI) to insoluble U(IV). During this experiment, planktonic biomass was sampled at various time points to quantitatively evaluate proteomes. In 2008, an acetateamended field experiment was again conducted in a similar manner to the 2007 experiment. As there was no comprehensive metagenome sequence available for use in proteomics analysis, we systematically evaluated 12 different organism genome sequences to generate sets of aggregate genomes, or pseudo-metagenomes , for supplying relative quantitative peptide and protein identifications. Proteomics results support previous observations of the dominance of Geobacteraceae during biostimulation using acetate as sole electron donor, and revealed a shift from an early stage of iron reduction to a late stage of iron reduction. Additionally,ashift from iron reduction to sulfate reduction was indicated by changes in the contribution of proteome information contributed by different organism genome sequences within the aggregate set. In addition, the comparison of proteome measurements made between the 2007 field experiment and 2008 field experiment revealed differences in proteome profiles. These differences may be the result of alterations in abundance and population structure within the planktonic biomass samples collected for analysis.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: Work for Others (WFO)
• DOE Contract Number: DE-AC05-00OR22725
• OSTI ID: 1000727
• Journal Information: Environmental Science & Technology, Vol. 44, Issue 23; ISSN 0013--936X
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
ABUNDANCE
ACETATES
AQUIFERS
BINDING ENERGY
BIOMASS
COMMUNITIES
ELECTRONS
IRON
PEPTIDES
PROTEINS
SULFATES
VALENCE