Elimination of formate production in Clostridium thermocellum
Abstract
We study the ability of Clostridium thermocellum to rapidly degrade cellulose and ferment resulting hydrolysis products into ethanol makes it a promising platform organism for cellulosic biofuel production via consolidated bioprocessing. Currently, however, ethanol yield are far below theoretical maximum due to branched product pathways that divert carbon and electrons towards formate, H2, lactate, acetate, and secreted amino acids. To redirect carbon and electron flux away from formate, pyruvate:formate lyase (pfl) and respective PFL-activating enzyme were deleted. Formate production in the resulting Δpfl strain was eliminated and acetate production decreased by 50% on both complex and defined medium. Growth rate of Δpfl decreased by 2.9-fold on defined medium and diauxic growth was observed on complex medium. Supplementation of defined medium with 2 mM formate restored Δpfl growth rate to 80% of the parent strain. Finally, we discuss the role of pfl in metabolic engineering strategies and C1 metabolism.
- Authors:
-
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); ; Dartmouth College, Hanover, NH (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1286847
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Industrial Microbiology and Biotechnology
- Additional Journal Information:
- Journal Volume: 42; Journal Issue: 9; Journal ID: ISSN 1367-5435
- Publisher:
- Springer
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; Cellulosic ethanol; Clostridium thermocellum; Pyruvate:formate lyase; Metabolic engineering; C1 metabolism
Citation Formats
Rydzak, Thomas, Lynd, Lee R., and Guss, Adam M. Elimination of formate production in Clostridium thermocellum. United States: N. p., 2015.
Web. doi:10.1007/s10295-015-1644-3.
Rydzak, Thomas, Lynd, Lee R., & Guss, Adam M. Elimination of formate production in Clostridium thermocellum. United States. https://doi.org/10.1007/s10295-015-1644-3
Rydzak, Thomas, Lynd, Lee R., and Guss, Adam M. Sat .
"Elimination of formate production in Clostridium thermocellum". United States. https://doi.org/10.1007/s10295-015-1644-3. https://www.osti.gov/servlets/purl/1286847.
@article{osti_1286847,
title = {Elimination of formate production in Clostridium thermocellum},
author = {Rydzak, Thomas and Lynd, Lee R. and Guss, Adam M.},
abstractNote = {We study the ability of Clostridium thermocellum to rapidly degrade cellulose and ferment resulting hydrolysis products into ethanol makes it a promising platform organism for cellulosic biofuel production via consolidated bioprocessing. Currently, however, ethanol yield are far below theoretical maximum due to branched product pathways that divert carbon and electrons towards formate, H2, lactate, acetate, and secreted amino acids. To redirect carbon and electron flux away from formate, pyruvate:formate lyase (pfl) and respective PFL-activating enzyme were deleted. Formate production in the resulting Δpfl strain was eliminated and acetate production decreased by 50% on both complex and defined medium. Growth rate of Δpfl decreased by 2.9-fold on defined medium and diauxic growth was observed on complex medium. Supplementation of defined medium with 2 mM formate restored Δpfl growth rate to 80% of the parent strain. Finally, we discuss the role of pfl in metabolic engineering strategies and C1 metabolism.},
doi = {10.1007/s10295-015-1644-3},
journal = {Journal of Industrial Microbiology and Biotechnology},
number = 9,
volume = 42,
place = {United States},
year = {Sat Jul 11 00:00:00 EDT 2015},
month = {Sat Jul 11 00:00:00 EDT 2015}
}
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The exometabolome of Clostridium thermocellum reveals overflow metabolism at high cellulose loading
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Increase in Ethanol Yield via Elimination of Lactate Production in an Ethanol-Tolerant Mutant of Clostridium thermocellum
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Works referencing / citing this record:
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Cellulosic ethanol production via consolidated bioprocessing at 75 °C by engineered Caldicellulosiruptor bescii
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Simultaneous achievement of high ethanol yield and titer in Clostridium thermocellum
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Improved growth rate in Clostridium thermocellum hydrogenase mutant via perturbed sulfur metabolism
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Expression of adhA from different organisms in Clostridium thermocellum
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- Biotechnology for Biofuels, Vol. 10, Issue 1
Clostridium thermocellum LL1210 pH homeostasis mechanisms informed by transcriptomics and metabolomics
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