An interface for the direct coupling of small liquid samples to AMS
Abstract
We describe the moving wire interface attached to the 1-MV AMS system at LLNL’s Center for Accelerator Mass Spectrometry for the analysis of nonvolatile liquid samples as either discrete drops or from the direct output of biochemical separatory instrumentation, such as high-performance liquid chromatography (HPLC). Discrete samples containing at least a few 10 s of nanograms of carbon and as little as 50 zmol 14C can be measured with a 3–5% precision in a few minutes. The dynamic range of our system spans approximately 3 orders in magnitude. Sample to sample memory is minimized by the use of fresh targets for each discrete sample or by minimizing the amount of carbon present in a peak generated by an HPLC containing a significant amount of 14C. As a result, liquid sample AMS provides a new technology to expand our biomedical AMS program by enabling the capability to measure low-level biochemicals in extremely small samples that would otherwise be inaccessible.
- Authors:
-
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Publication Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1266673
- Alternate Identifier(s):
- OSTI ID: 1246215
- Report Number(s):
- LLNL-JRNL-664719
Journal ID: ISSN 0168-583X
- Grant/Contract Number:
- AC52-07NA27344
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nuclear Instruments and Methods in Physics Research. Section B, Beam Interactions with Materials and Atoms
- Additional Journal Information:
- Journal Volume: 361; Journal Issue: C; Journal ID: ISSN 0168-583X
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 47 OTHER INSTRUMENTATION; 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; accelerator mass spectrometry; CO2; HPLC interfaces; 14C
Citation Formats
Ognibene, T. J., Thomas, A. T., Daley, P. F., Bench, G., and Turteltaub, K. W. An interface for the direct coupling of small liquid samples to AMS. United States: N. p., 2015.
Web. doi:10.1016/j.nimb.2015.05.018.
Ognibene, T. J., Thomas, A. T., Daley, P. F., Bench, G., & Turteltaub, K. W. An interface for the direct coupling of small liquid samples to AMS. United States. https://doi.org/10.1016/j.nimb.2015.05.018
Ognibene, T. J., Thomas, A. T., Daley, P. F., Bench, G., and Turteltaub, K. W. Thu .
"An interface for the direct coupling of small liquid samples to AMS". United States. https://doi.org/10.1016/j.nimb.2015.05.018. https://www.osti.gov/servlets/purl/1266673.
@article{osti_1266673,
title = {An interface for the direct coupling of small liquid samples to AMS},
author = {Ognibene, T. J. and Thomas, A. T. and Daley, P. F. and Bench, G. and Turteltaub, K. W.},
abstractNote = {We describe the moving wire interface attached to the 1-MV AMS system at LLNL’s Center for Accelerator Mass Spectrometry for the analysis of nonvolatile liquid samples as either discrete drops or from the direct output of biochemical separatory instrumentation, such as high-performance liquid chromatography (HPLC). Discrete samples containing at least a few 10 s of nanograms of carbon and as little as 50 zmol 14C can be measured with a 3–5% precision in a few minutes. The dynamic range of our system spans approximately 3 orders in magnitude. Sample to sample memory is minimized by the use of fresh targets for each discrete sample or by minimizing the amount of carbon present in a peak generated by an HPLC containing a significant amount of 14C. As a result, liquid sample AMS provides a new technology to expand our biomedical AMS program by enabling the capability to measure low-level biochemicals in extremely small samples that would otherwise be inaccessible.},
doi = {10.1016/j.nimb.2015.05.018},
journal = {Nuclear Instruments and Methods in Physics Research. Section B, Beam Interactions with Materials and Atoms},
number = C,
volume = 361,
place = {United States},
year = {Thu May 28 00:00:00 EDT 2015},
month = {Thu May 28 00:00:00 EDT 2015}
}
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Works referenced in this record:
A High-Throughput Method for the Conversion of CO 2 Obtained from Biochemical Samples to Graphite in Septa-Sealed Vials for Quantification of 14 C via Accelerator Mass Spectrometry
journal, May 2003
- Ognibene, Ted J.; Bench, Graham; Vogel, John S.
- Analytical Chemistry, Vol. 75, Issue 9
Installation of hybrid ion source on the 1-MV LLNL BioAMS spectrometer
journal, January 2013
- Ognibene, T. J.; Salazar, G. A.
- Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, Vol. 294
Ultrahigh Efficiency Moving Wire Combustion Interface for Online Coupling of High-Performance Liquid Chromatography (HPLC)
journal, December 2011
- Thomas, Avi T.; Ognibene, Ted; Daley, Paul
- Analytical Chemistry, Vol. 83, Issue 24
Directly Coupled High-Performance Liquid Chromatography–Accelerator Mass Spectrometry Measurement of Chemically Modified Protein and Peptides
journal, March 2013
- Thomas, Avi T.; Stewart, Benjamin J.; Ognibene, Ted J.
- Analytical Chemistry, Vol. 85, Issue 7
Isotope-Ratio-Monitoring Liquid Chromatography Mass Spectrometry (IRM-LCMS): First Results from a Moving Wire Interface System
journal, August 1996
- Brand, W. A.; Dobberstein, P.
- Isotopes in Environmental and Health Studies, Vol. 32, Issue 2-3
High-precision liquid chromatography-combustion isotope ratio mass spectrometry
journal, December 1993
- Caimi, Richard J.; Brenna, J. Thomas.
- Analytical Chemistry, Vol. 65, Issue 23
Carbon-isotopic analysis of microbial cells sorted by flow cytometry
journal, March 2007
- Eek, K. M.; Sessions, A. L.; Lies, D. P.
- Geobiology, Vol. 5, Issue 1
Moving-Wire Device for Carbon Isotopic Analyses of Nanogram Quantities of Nonvolatile Organic Carbon
journal, October 2005
- Sessions, Alex L.; Sylva, Sean P.; Hayes, John M.
- Analytical Chemistry, Vol. 77, Issue 20
Gettering of Gas by Titanium
journal, December 1955
- Stout, Virgil L.; Gibbons, Martin D.
- Journal of Applied Physics, Vol. 26, Issue 12
High-sensitivity liquid chromatography-combustion isotope ratio mass spectrometry of fat-soluble vitamins
journal, March 1995
- Caimi, Richard J.; Brenna, J. Thomas
- Journal of Mass Spectrometry, Vol. 30, Issue 3
Design of a secondary ionization target for direct production of a C− beam from CO2 pulses for online AMS
journal, January 2013
- Salazar, Gary; Ognibene, Ted
- Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, Vol. 294
Improving a gas ion source for 14C AMS
journal, January 2013
- Fahrni, S. M.; Wacker, L.; Synal, H. -A.
- Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, Vol. 294
Interaction of titanium and vanadium with carbon dioxide in heating
journal, January 1986
- Vlasyuk, R. Z.; Kurovskii, V. Ya.; Lyapunov, A. P.
- Soviet Powder Metallurgy and Metal Ceramics, Vol. 25, Issue 1
Works referencing / citing this record:
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journal, May 2019
- Malfatti, Michael A.; Buchholz, Bruce A.; Enright, Heather A.
- Toxics, Vol. 7, Issue 2
Radiocarbon Tracers in Toxicology and Medicine: Recent Advances in Technology and Science
journal, May 2019
- Malfatti, Michael A.; Buchholz, Bruce A.; Enright, Heather A.
- Toxics, Vol. 7, Issue 2