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Title: Scattering from phase-separated vesicles. I. An analytical form factor for multiple static domains

Abstract

This is the first in a series of studies considering elastic scattering from laterally heterogeneous lipid vesicles containing multiple domains. Unique among biophysical tools, small-angle neutron scattering can in principle give detailed information about the size, shape and spatial arrangement of domains. A general theory for scattering from laterally heterogeneous vesicles is presented, and the analytical form factor for static domains with arbitrary spatial configuration is derived, including a simplification for uniformly sized round domains. The validity of the model, including series truncation effects, is assessed by comparison with simulated data obtained from a Monte Carlo method. Several aspects of the analytical solution for scattering intensity are discussed in the context of small-angle neutron scattering data, including the effect of varying domain size and number, as well as solvent contrast. Finally, the analysis indicates that effects of domain formation are most pronounced when the vesicle's average scattering length density matches that of the surrounding solvent.

Authors:
 [1];  [2];  [3]
+ Show Author Affiliations
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biology and Soft Matter Division
  2. Canadian Nuclear Lab., Chalk River, ON (Canada)
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biology and Soft Matter Division; Univ. of Tennessee, Knoxville, TN (United States). Dept. of Physics and Astronomy; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Inst. for Neutron Sciences; Brock Univ., St. Catharines, ON (Canada). Dept. of Physics
Publication Date:
Research Org.:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1265605
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Applied Crystallography (Online)
Additional Journal Information:
Journal Name: Journal of Applied Crystallography (Online); Journal Volume: 48; Journal Issue: 5; Journal ID: ISSN 1600-5767
Publisher:
International Union of Crystallography
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 47 OTHER INSTRUMENTATION; bilayer phases; lipid raft; liquid ordered; liquid disordered; nanodomains

Citation Formats

Heberle, Frederick A., Anghel, Vinicius N. P., and Katsaras, John. Scattering from phase-separated vesicles. I. An analytical form factor for multiple static domains. United States: N. p., 2015. Web. doi:10.1107/S160057671501362X.
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Heberle, Frederick A., Anghel, Vinicius N. P., & Katsaras, John. Scattering from phase-separated vesicles. I. An analytical form factor for multiple static domains. United States. https://doi.org/10.1107/S160057671501362X
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Heberle, Frederick A., Anghel, Vinicius N. P., and Katsaras, John. Tue . "Scattering from phase-separated vesicles. I. An analytical form factor for multiple static domains". United States. https://doi.org/10.1107/S160057671501362X. https://www.osti.gov/servlets/purl/1265605.
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@article{osti_1265605,
title = {Scattering from phase-separated vesicles. I. An analytical form factor for multiple static domains},
author = {Heberle, Frederick A. and Anghel, Vinicius N. P. and Katsaras, John},
abstractNote = {This is the first in a series of studies considering elastic scattering from laterally heterogeneous lipid vesicles containing multiple domains. Unique among biophysical tools, small-angle neutron scattering can in principle give detailed information about the size, shape and spatial arrangement of domains. A general theory for scattering from laterally heterogeneous vesicles is presented, and the analytical form factor for static domains with arbitrary spatial configuration is derived, including a simplification for uniformly sized round domains. The validity of the model, including series truncation effects, is assessed by comparison with simulated data obtained from a Monte Carlo method. Several aspects of the analytical solution for scattering intensity are discussed in the context of small-angle neutron scattering data, including the effect of varying domain size and number, as well as solvent contrast. Finally, the analysis indicates that effects of domain formation are most pronounced when the vesicle's average scattering length density matches that of the surrounding solvent.},
doi = {10.1107/S160057671501362X},
journal = {Journal of Applied Crystallography (Online)},
number = 5,
volume = 48,
place = {United States},
year = {Tue Aug 18 00:00:00 EDT 2015},
month = {Tue Aug 18 00:00:00 EDT 2015}
}
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https://doi.org/10.1107/S160057671501362X
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