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Title: Comparative analysis of the ability of Clostridium clariflavum strains and Clostridium thermocellumto utilize hemicellulose and unpretreated plant material

Abstract

Among themophilic consolidated bioprocessing (CBP) candidate organisms, environmental isolates of Clostridium clariflavum have demonstrated the ability to grow on xylan, and the genome of C. clariflavum DSM 19732 has revealed a number of mechanisms that foster solubilization of hemicellulose that are distinctive relative to the model cellulolytic thermophile Clostridium thermocellum. Growth experiments on xylan, xylooligosaccharides, and xylose reveal that C. clariflavum strains are able to completely break down xylan to xylose and that the environmental strain C. clariflavum sp. 4-2a is able to grow on monomeric xylose. C. clariflavum strains were able to utilize a larger proportion of unpretreated switchgrass, and solubilize a higher proportion of glucan, xylan, and arabinan, with strain 4-2a reaching the highest extent of solubilization of these components (64.7 to 69.4%) compared to C. thermocellum (29.5 to 42.5%). In addition, glycome immunoanalyses of residual plant biomass reveal differences in the extent of degradation of easily accessible xylans, with C. clariflavum strains having increased solubilization of this fraction of xylans relative to C. thermocellum. In conclusion, C. clariflavum strains exhibit higher activity than C. thermocellum in the breakdown of hemicellulose and are capable of degrading xylan to xylooligomers and xylose. This capability seems to also play amore » role in the higher levels of utilization of unpretreated plant material.« less

Authors:
 [1];  [2];  [3];  [2];  [3]
+ Show Author Affiliations
  1. Dartmouth College, Hanover, NH (United States). Thayer School of Engineering; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Hofstra Univ., Hempstead, NY (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Univ. of Georgia, Athens, GA (United States)
  3. Dartmouth College, Hanover, NH (United States). Thayer School of Engineering; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
Publication Date:
Research Org.:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1259857
Resource Type:
Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CBP; Clostridium thermocellum; Clostridium clariflavum; Hemicellulose; Switchgrass

Citation Formats

Izquierdo, Javier A., Pattathil, Sivakumar, Guseva, Anna, Hahn, Michael G., and Lynd, Lee R. Comparative analysis of the ability of Clostridium clariflavum strains and Clostridium thermocellumto utilize hemicellulose and unpretreated plant material. United States: N. p., 2014. Web. doi:10.1186/s13068-014-0136-4.
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Izquierdo, Javier A., Pattathil, Sivakumar, Guseva, Anna, Hahn, Michael G., & Lynd, Lee R. Comparative analysis of the ability of Clostridium clariflavum strains and Clostridium thermocellumto utilize hemicellulose and unpretreated plant material. United States. https://doi.org/10.1186/s13068-014-0136-4
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Izquierdo, Javier A., Pattathil, Sivakumar, Guseva, Anna, Hahn, Michael G., and Lynd, Lee R. Tue . "Comparative analysis of the ability of Clostridium clariflavum strains and Clostridium thermocellumto utilize hemicellulose and unpretreated plant material". United States. https://doi.org/10.1186/s13068-014-0136-4. https://www.osti.gov/servlets/purl/1259857.
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@article{osti_1259857,
title = {Comparative analysis of the ability of Clostridium clariflavum strains and Clostridium thermocellumto utilize hemicellulose and unpretreated plant material},
author = {Izquierdo, Javier A. and Pattathil, Sivakumar and Guseva, Anna and Hahn, Michael G. and Lynd, Lee R.},
abstractNote = {Among themophilic consolidated bioprocessing (CBP) candidate organisms, environmental isolates of Clostridium clariflavum have demonstrated the ability to grow on xylan, and the genome of C. clariflavum DSM 19732 has revealed a number of mechanisms that foster solubilization of hemicellulose that are distinctive relative to the model cellulolytic thermophile Clostridium thermocellum. Growth experiments on xylan, xylooligosaccharides, and xylose reveal that C. clariflavum strains are able to completely break down xylan to xylose and that the environmental strain C. clariflavum sp. 4-2a is able to grow on monomeric xylose. C. clariflavum strains were able to utilize a larger proportion of unpretreated switchgrass, and solubilize a higher proportion of glucan, xylan, and arabinan, with strain 4-2a reaching the highest extent of solubilization of these components (64.7 to 69.4%) compared to C. thermocellum (29.5 to 42.5%). In addition, glycome immunoanalyses of residual plant biomass reveal differences in the extent of degradation of easily accessible xylans, with C. clariflavum strains having increased solubilization of this fraction of xylans relative to C. thermocellum. In conclusion, C. clariflavum strains exhibit higher activity than C. thermocellum in the breakdown of hemicellulose and are capable of degrading xylan to xylooligomers and xylose. This capability seems to also play a role in the higher levels of utilization of unpretreated plant material.},
doi = {10.1186/s13068-014-0136-4},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 7,
place = {United States},
year = {Tue Nov 18 00:00:00 EST 2014},
month = {Tue Nov 18 00:00:00 EST 2014}
}
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https://doi.org/10.1186/s13068-014-0136-4
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Microbial production of fatty-acid-derived fuels and chemicals from plant biomass
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Fungal cellulases and complexed cellulosomal enzymes exhibit synergistic mechanisms in cellulose deconstruction
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    Works referencing / citing this record:

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    Physical and chemical differences between one-stage and two-stage hydrothermal pretreated hardwood substrates for use in cellulosic ethanol production
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    Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover
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    Genomics and prevalence of bacterial and archaeal isolates from biogas-producing microbiomes
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    Development and characterization of stable anaerobic thermophilic methanogenic microbiomes fermenting switchgrass at decreasing residence times
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    Immunological Approaches to Biomass Characterization and Utilization
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    • Pattathil, Sivakumar; Avci, Utku; Zhang, Tiantian
    • Frontiers in Bioengineering and Biotechnology, Vol. 3
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    Pentose sugars inhibit metabolism and increase expression of an AgrD-type cyclic pentapeptide in Clostridium thermocellum
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    Clostridium clariflavum: Key Cellulosome Players Are Revealed by Proteomic Analysis
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    journal, January 2016

    • Paye, Julie M. D.; Guseva, Anna; Hammer, Sarah K.
    • Biotechnology for Biofuels, Vol. 9, Issue 1
    • DOI: 10.1186/s13068-015-0412-y

    Physical and chemical differences between one-stage and two-stage hydrothermal pretreated hardwood substrates for use in cellulosic ethanol production
    journal, February 2016

    • Guilliams, Andrew; Pattathil, Sivakumar; Willies, Deidre
    • Biotechnology for Biofuels, Vol. 9, Issue 1
    • DOI: 10.1186/s13068-016-0446-9

    Cellulosic ethanol production via consolidated bioprocessing by a novel thermophilic anaerobic bacterium isolated from a Himalayan hot spring
    journal, March 2017

    • Singh, Nisha; Mathur, Anshu S.; Tuli, Deepak K.
    • Biotechnology for Biofuels, Vol. 10, Issue 1
    • DOI: 10.1186/s13068-017-0756-6

    Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover
    journal, March 2017

    • Gunawan, Christa; Xue, Saisi; Pattathil, Sivakumar
    • Biotechnology for Biofuels, Vol. 10, Issue 1
    • DOI: 10.1186/s13068-017-0757-5

    Development and characterization of stable anaerobic thermophilic methanogenic microbiomes fermenting switchgrass at decreasing residence times
    journal, September 2018

    • Liang, Xiaoyu; Whitham, Jason M.; Holwerda, Evert K.
    • Biotechnology for Biofuels, Vol. 11, Issue 1
    • DOI: 10.1186/s13068-018-1238-1

    Immunological Approaches to Biomass Characterization and Utilization
    journal, October 2015

    • Pattathil, Sivakumar; Avci, Utku; Zhang, Tiantian
    • Frontiers in Bioengineering and Biotechnology, Vol. 3
    • DOI: 10.3389/fbioe.2015.00173
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