BdCESA7, BdCESA8, and BdPMT utility promoter constructs for targeted expression to secondary cell-wall-forming cells of grasses
Abstract
© 2016 Petrik, Cass, Padmakshan, Foster, Vogel, Karlen, Ralph and Sedbrook. Utility vectors with promoters that confer desired spatial and temporal expression patterns are useful tools for studying gene and cellular function and for industrial applications. To target the expression of DNA sequences of interest to cells forming plant secondary cell walls, which generate most of the vegetative biomass, upstream regulatory sequences of the Brachypodium distachyon lignin biosynthetic gene BdPMT and the cellulose synthase genes BdCESA7 and BdCESA8 were isolated and cloned into binary vectors designed for Agrobacterium-mediated transformation of monocots. Expression patterns were assessed using the β-glucuronidase gene GUSPlus and X-glucuronide staining. All three promoters showed strong expression levels in stem tissue at the base of internodes where cell wall deposition is most active, in both vascular bundle xylem vessels and tracheids, and in interfascicular tissues, with expression less pronounced in developmentally older tissues. In leaves, BdCESA7 and BdCESA8 promoter-driven expression was strongest in leaf veins, leaf margins, and trichomes; relatively weaker and patchy expression was observed in the epidermis. BdPMT promoter-driven expression was similar to the BdCESA promoters expression patterns, including strong expression in trichomes. The intensity and extent of GUS staining varied considerably between transgenic lines, suggestingmore »
- Authors:
-
- Illinois State Univ., Normal, IL (United States); Univ. of Wisconsin-Madison, Madison, WI (United States). DOE Great Lakes Bioenergy Research Center
- Univ. of Wisconsin-Madison, Madison, WI (United States). DOE Great Lakes Bioenergy Research Center
- Michigan State Univ., East Lansing, MI (United States). DOE Great Lakes Bioenergy Research Center
- DOE Joint Genome Institute, Walnut Creek, CA (United States)
- Univ. of Wisconsin-Madison, Madison, WI (United States). DOE Great Lakes Bioenergy Research Center; Univ. of Wisconsin-Madison, Madison, WI (United States). Dept.of Biochemistry
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1257265
- Alternate Identifier(s):
- OSTI ID: 1379077
- Grant/Contract Number:
- FC02–07ER64494; AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Frontiers in Plant Science
- Additional Journal Information:
- Journal Volume: 7; Journal ID: ISSN 1664-462X
- Publisher:
- Frontiers Research Foundation
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; binary vectors; Brachypodium; cellulose; lignin; monocot; p-coumarate; tissue-specific expression
Citation Formats
Petrik, Deborah L., Cass, Cynthia L., Padmakshan, Dharshana, Foster, Cliff E., Vogel, John P., Karlen, Steven D., Ralph, John, and Sedbrook, John C. BdCESA7, BdCESA8, and BdPMT utility promoter constructs for targeted expression to secondary cell-wall-forming cells of grasses. United States: N. p., 2016.
Web. doi:10.3389/fpls.2016.00055.
Petrik, Deborah L., Cass, Cynthia L., Padmakshan, Dharshana, Foster, Cliff E., Vogel, John P., Karlen, Steven D., Ralph, John, & Sedbrook, John C. BdCESA7, BdCESA8, and BdPMT utility promoter constructs for targeted expression to secondary cell-wall-forming cells of grasses. United States. https://doi.org/10.3389/fpls.2016.00055
Petrik, Deborah L., Cass, Cynthia L., Padmakshan, Dharshana, Foster, Cliff E., Vogel, John P., Karlen, Steven D., Ralph, John, and Sedbrook, John C. Thu .
"BdCESA7, BdCESA8, and BdPMT utility promoter constructs for targeted expression to secondary cell-wall-forming cells of grasses". United States. https://doi.org/10.3389/fpls.2016.00055. https://www.osti.gov/servlets/purl/1257265.
@article{osti_1257265,
title = {BdCESA7, BdCESA8, and BdPMT utility promoter constructs for targeted expression to secondary cell-wall-forming cells of grasses},
author = {Petrik, Deborah L. and Cass, Cynthia L. and Padmakshan, Dharshana and Foster, Cliff E. and Vogel, John P. and Karlen, Steven D. and Ralph, John and Sedbrook, John C.},
abstractNote = {© 2016 Petrik, Cass, Padmakshan, Foster, Vogel, Karlen, Ralph and Sedbrook. Utility vectors with promoters that confer desired spatial and temporal expression patterns are useful tools for studying gene and cellular function and for industrial applications. To target the expression of DNA sequences of interest to cells forming plant secondary cell walls, which generate most of the vegetative biomass, upstream regulatory sequences of the Brachypodium distachyon lignin biosynthetic gene BdPMT and the cellulose synthase genes BdCESA7 and BdCESA8 were isolated and cloned into binary vectors designed for Agrobacterium-mediated transformation of monocots. Expression patterns were assessed using the β-glucuronidase gene GUSPlus and X-glucuronide staining. All three promoters showed strong expression levels in stem tissue at the base of internodes where cell wall deposition is most active, in both vascular bundle xylem vessels and tracheids, and in interfascicular tissues, with expression less pronounced in developmentally older tissues. In leaves, BdCESA7 and BdCESA8 promoter-driven expression was strongest in leaf veins, leaf margins, and trichomes; relatively weaker and patchy expression was observed in the epidermis. BdPMT promoter-driven expression was similar to the BdCESA promoters expression patterns, including strong expression in trichomes. The intensity and extent of GUS staining varied considerably between transgenic lines, suggesting that positional effects influenced promoter activity. Introducing the BdPMT and BdCESA8 Open Reading Frames into BdPMT and BdCESA8 utility promoter binary vectors, respectively, and transforming those constructs into Brachypodium pmt and cesa8 loss-of-function mutants resulted in rescue of the corresponding mutant phenotypes. This work therefore validates the functionality of these utility promoter binary vectors for use in Brachypodium and likely other grass species. The identification, in Bdcesa8-1 T-DNA mutant stems, of an 80% reduction in crystalline cellulose levels confirms that the BdCESA8 gene is a secondary-cell-wall-forming cellulose synthase.},
doi = {10.3389/fpls.2016.00055},
journal = {Frontiers in Plant Science},
number = ,
volume = 7,
place = {United States},
year = {Thu Feb 04 00:00:00 EST 2016},
month = {Thu Feb 04 00:00:00 EST 2016}
}
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Works referenced in this record:
The biosynthesis, composition and assembly of the outer pollen wall: A tough case to crack
journal, May 2015
- Quilichini, Teagen D.; Grienenberger, Etienne; Douglas, Carl J.
- Phytochemistry, Vol. 113
TM2, a novel strong matrix attachment region isolated from tobacco, increases transgene expression in transgenic rice calli and plants
journal, January 2005
- Xue, Hua; Yang, Yu-Tao; Wu, Chang-Ai
- Theoretical and Applied Genetics, Vol. 110, Issue 4
The biosynthesis, composition and assembly of the outer pollen wall: A tough case to crack
journal, May 2015
- Quilichini, Teagen D.; Grienenberger, Etienne; Douglas, Carl J.
- Phytochemistry, Vol. 113
Lignin bioengineering
journal, April 2014
- Eudes, Aymerick; Liang, Yan; Mitra, Prajakta
- Current Opinion in Biotechnology, Vol. 26
A Set of Modular Binary Vectors for Transformation of Cereals
journal, November 2007
- Himmelbach, Axel; Zierold, Uwe; Hensel, Götz
- Plant Physiology, Vol. 145, Issue 4
Lignin modification improves fermentable sugar yields for biofuel production
journal, June 2007
- Chen, Fang; Dixon, Richard A.
- Nature Biotechnology, Vol. 25, Issue 7, p. 759-761
Genetic evidence for three unique components in primary cell-wall cellulose synthase complexes in Arabidopsis
journal, September 2007
- Persson, S.; Paredez, A.; Carroll, A.
- Proceedings of the National Academy of Sciences, Vol. 104, Issue 39
PlaNet: Combined Sequence and Expression Comparisons across Plant Networks Derived from Seven Species
journal, March 2011
- Mutwil, Marek; Klie, Sebastian; Tohge, Takayuki
- The Plant Cell, Vol. 23, Issue 3
Lignin modification improves fermentable sugar yields for biofuel production
journal, June 2007
- Chen, Fang; Dixon, Richard A.
- Nature Biotechnology, Vol. 25, Issue 7, p. 759-761
Regulation of secondary wall synthesis and cell death by NAC transcription factors in the monocot Brachypodium distachyon
journal, February 2013
- Valdivia, Elene R.; Herrera, María Teresa; Gianzo, Cristina
- Journal of Experimental Botany, Vol. 64, Issue 5
High-efficiency Agrobacterium-mediated transformation of Brachypodium distachyon inbred line Bd21-3
journal, November 2007
- Vogel, John; Hill, Theresa
- Plant Cell Reports, Vol. 27, Issue 3
Assaying chimeric genes in plants: The GUS gene fusion system
journal, December 1987
- Jefferson, Richard A.
- Plant Molecular Biology Reporter, Vol. 5, Issue 4
Lignin bioengineering
journal, April 2014
- Eudes, Aymerick; Liang, Yan; Mitra, Prajakta
- Current Opinion in Biotechnology, Vol. 26
A Set of Modular Binary Vectors for Transformation of Cereals
journal, November 2007
- Himmelbach, Axel; Zierold, Uwe; Hensel, Götz
- Plant Physiology, Vol. 145, Issue 4
Identification of Grass-specific Enzyme That Acylates Monolignols with p-Coumarate
journal, January 2012
- Withers, Saunia; Lu, Fachuang; Kim, Hoon
- Journal of Biological Chemistry, Vol. 287, Issue 11, p. 8347-8355
Regulation of Dormancy in Barley by Blue Light and After-Ripening: Effects on Abscisic Acid and Gibberellin Metabolism
journal, April 2008
- Gubler, Frank; Hughes, Trijntje; Waterhouse, Peter
- Plant Physiology, Vol. 147, Issue 2
Expression Enhancement of a Rice Polyubiquitin Gene Promoter
journal, January 2006
- Sivamani, Elumalai; Qu, Rongda
- Plant Molecular Biology, Vol. 60, Issue 2, p. 225-239
Brachypodium distachyon promoters as efficient building blocks for transgenic research in maize
journal, April 2012
- Coussens, Griet; Aesaert, Stijn; Verelst, Wim
- Journal of Experimental Botany, Vol. 63, Issue 11
The wheat HMW-glutenin 1Dy10 gene promoter controls endosperm expression in Brachypodium distachyon
journal, December 2013
- Thilmony, Roger; Guttman, Mara E.; Lin, Jeanie W.
- GM Crops & Food, Vol. 5, Issue 1
The wheat HMW-glutenin 1Dy10 gene promoter controls endosperm expression in Brachypodium distachyon
journal, December 2013
- Thilmony, Roger; Guttman, Mara E.; Lin, Jeanie W.
- GM Crops & Food, Vol. 5, Issue 1
A new method for isolating large quantities of Arabidopsis trichomes for transcriptome, cell wall and other types of analyses
journal, November 2008
- Marks, M. David; Betancur, Lissete; Gilding, Edward
- The Plant Journal, Vol. 56, Issue 3
Regulation of secondary wall synthesis and cell death by NAC transcription factors in the monocot Brachypodium distachyon
journal, February 2013
- Valdivia, Elene R.; Herrera, María Teresa; Gianzo, Cristina
- Journal of Experimental Botany, Vol. 64, Issue 5
Brachypodium distachyon promoters as efficient building blocks for transgenic research in maize
journal, April 2012
- Coussens, Griet; Aesaert, Stijn; Verelst, Wim
- Journal of Experimental Botany, Vol. 63, Issue 11
Engineering the Oryza sativa cell wall with rice NAC transcription factors regulating secondary wall formation
journal, January 2013
- Yoshida, Kouki; Sakamoto, Shingo; Kawai, Tetsushi
- Frontiers in Plant Science, Vol. 4
Genetic Complexity of Cellulose Synthase A Gene Function in Arabidopsis Embryogenesis
journal, December 2002
- Beeckman, Tom; Przemeck, Gerhard K. H.; Stamatiou, George
- Plant Physiology, Vol. 130, Issue 4
Three Distinct Rice Cellulose Synthase Catalytic Subunit Genes Required for Cellulose Synthesis in the Secondary Wall
journal, August 2003
- Tanaka, Katsuyuki; Murata, Kazumasa; Yamazaki, Muneo
- Plant Physiology, Vol. 133, Issue 1
Metabolic engineering of novel lignin in biomass crops
journal, October 2012
- Vanholme, Ruben; Morreel, Kris; Darrah, Chiarina
- New Phytologist, Vol. 196, Issue 4
Monolignol Ferulate Transferase Introduces Chemically Labile Linkages into the Lignin Backbone
journal, April 2014
- Wilkerson, C. G.; Mansfield, S. D.; Lu, F.
- Science, Vol. 344, Issue 6179, p. 90-93
Genetic evidence for three unique components in primary cell-wall cellulose synthase complexes in Arabidopsis
journal, September 2007
- Persson, S.; Paredez, A.; Carroll, A.
- Proceedings of the National Academy of Sciences, Vol. 104, Issue 39
p -Coumaroyl-CoA:monolignol transferase (PMT) acts specifically in the lignin biosynthetic pathway in Brachypodium distachyon
journal, February 2014
- Petrik, Deborah L.; Karlen, Steven D.; Cass, Cynthia L.
- The Plant Journal, Vol. 77, Issue 5
Characterization of a plant scaffold attachment region in a DNA fragment that normalizes transgene expression in tobacco.
journal, April 1992
- Breyne, P.; van Montagu, M.; Depicker, N.
- The Plant Cell, Vol. 4, Issue 4
Location of cellulose and callose in pollen tubes and grains of Nicotiana tabacum
journal, August 1998
- Ferguson, C.; Teeri, T. T.; Siika-aho, M.
- Planta, Vol. 206, Issue 3
Cellulosic Biofuels
journal, June 2009
- Carroll, Andrew; Somerville, Chris
- Annual Review of Plant Biology, Vol. 60, Issue 1
Improved Vectors for Agrobacterium Tumefaciens-Mediated Transformation of Monocot Plants
journal, August 1998
- Wang, M.; Li, Z.; Matthews, Peter R.
- Acta Horticulturae, Issue 461
Characterization of a Plant Scaffold Attachment Region in a DNA Fragment That Normalizes Transgene Expression in Tobacco
journal, April 1992
- Breyne, Peter; Montagu, Marc Van; Depicker, Ann
- The Plant Cell, Vol. 4, Issue 4
BRITTLE CULM1, Which Encodes a COBRA-Like Protein, Affects the Mechanical Properties of Rice Plants
journal, August 2003
- Li, Yunhai; Qian, Qian; Zhou, Yihua
- The Plant Cell, Vol. 15, Issue 9
Quantitative transient gene expression: Comparison of the promoters for maize polyubiquitin1, rice actin1, maize-derivedEmu andCaMV 35S in cells of barley, maize and tobacco
journal, July 1994
- Schledzewski, Kai; Mendel, Ralf R.
- Transgenic Research, Vol. 3, Issue 4
Perturbation of Brachypodium distachyon CELLULOSE SYNTHASE A4 or 7 results in abnormal cell walls
journal, January 2013
- Handakumbura, Pubudu P.; Matos, Dominick A.; Osmont, Karen S.
- BMC Plant Biology, Vol. 13, Issue 1
Generation and Characterization of the Western Regional Research Center Brachypodium T-DNA Insertional Mutant Collection
journal, September 2012
- Bragg, Jennifer N.; Wu, Jiajie; Gordon, Sean P.
- PLoS ONE, Vol. 7, Issue 9
Engineering secondary cell wall deposition in plants
journal, November 2012
- Yang, Fan; Mitra, Prajakta; Zhang, Ling
- Plant Biotechnology Journal, Vol. 11, Issue 3
Perturbation of Brachypodium distachyon CELLULOSE SYNTHASE A4 or 7 results in abnormal cell walls
journal, January 2013
- Handakumbura, Pubudu P.; Matos, Dominick A.; Osmont, Karen S.
- BMC Plant Biology, Vol. 13, Issue 1
Loosening lignin's grip on biofuel production
journal, July 2007
- Chapple, Clint; Ladisch, Michael; Meilan, Rick
- Nature Biotechnology, Vol. 25, Issue 7, p. 746-748
Loosening lignin's grip on biofuel production
journal, July 2007
- Chapple, Clint; Ladisch, Michael; Meilan, Rick
- Nature Biotechnology, Vol. 25, Issue 7, p. 746-748
The Maize macrohairless1 Locus Specifically Promotes Leaf Blade Macrohair Initiation and Responds to Factors Regulating Leaf Identity
journal, March 2004
- Moose, Stephen P.; Lauter, Nick; Carlson, Shawn R.
- Genetics, Vol. 166, Issue 3
The Maize macrohairless1 Locus Specifically Promotes Leaf Blade Macrohair Initiation and Responds to Factors Regulating Leaf Identity
journal, March 2004
- Moose, Stephen P.; Lauter, Nick; Carlson, Shawn R.
- Genetics, Vol. 166, Issue 3
Generation and Characterization of the Western Regional Research Center Brachypodium T-DNA Insertional Mutant Collection
journal, September 2012
- Bragg, Jennifer N.; Wu, Jiajie; Gordon, Sean P.
- PLoS ONE, Vol. 7, Issue 9
Switchgrass (Panicum virgatum L.) polyubiquitin gene (PvUbi1 and PvUbi2) promoters for use in plant transformation
journal, July 2011
- Mann, David GJ; King, Zachary R.; Liu, Wusheng
- BMC Biotechnology, Vol. 11, Issue 1
Genetic Complexity of Cellulose Synthase A Gene Function in Arabidopsis Embryogenesis
journal, December 2002
- Beeckman, Tom; Przemeck, Gerhard K. H.; Stamatiou, George
- Plant Physiology, Vol. 130, Issue 4
Three Distinct Rice Cellulose Synthase Catalytic Subunit Genes Required for Cellulose Synthesis in the Secondary Wall
journal, August 2003
- Tanaka, Katsuyuki; Murata, Kazumasa; Yamazaki, Muneo
- Plant Physiology, Vol. 133, Issue 1
Switchgrass (Panicum virgatum L.) polyubiquitin gene (PvUbi1 and PvUbi2) promoters for use in plant transformation
journal, July 2011
- Mann, David GJ; King, Zachary R.; Liu, Wusheng
- BMC Biotechnology, Vol. 11, Issue 1
Effect of nuclear matrix attachment regions on transgene expression in tobacco plants.
journal, September 2001
- Nowak, W.; Gawłowska, M.; Jarmołowski, A.
- Acta Biochimica Polonica, Vol. 48, Issue 3
A BAHD acyltransferase is expressed in the tapetum of Arabidopsis anthers and is involved in the synthesis of hydroxycinnamoyl spermidines
journal, April 2009
- Grienenberger, Etienne; Besseau, Sébastien; Geoffroy, Pierrette
- The Plant Journal, Vol. 58, Issue 2
Cell Walls and the Developmental Anatomy of the Brachypodium distachyon Stem Internode
journal, November 2013
- Matos, Dominick A.; Whitney, Ian P.; Harrington, Michael J.
- PLoS ONE, Vol. 8, Issue 11
Cellulosic Biofuels
journal, June 2009
- Carroll, Andrew; Somerville, Chris
- Annual Review of Plant Biology, Vol. 60, Issue 1
Engineering secondary cell wall deposition in plants
journal, November 2012
- Yang, Fan; Mitra, Prajakta; Zhang, Ling
- Plant Biotechnology Journal, Vol. 11, Issue 3
Regulation of secondary cell wall biosynthesis by poplar R2R3 MYB transcription factor PtrMYB152 in Arabidopsis
journal, May 2014
- Wang, Shucai; Li, Eryang; Porth, Ilga
- Scientific Reports, Vol. 4, Issue 1
Regulation of Dormancy in Barley by Blue Light and After-Ripening: Effects on Abscisic Acid and Gibberellin Metabolism
journal, April 2008
- Gubler, Frank; Hughes, Trijntje; Waterhouse, Peter
- Plant Physiology, Vol. 147, Issue 2
Expression Enhancement of a Rice Polyubiquitin Gene Promoter
journal, January 2006
- Sivamani, Elumalai; Qu, Rongda
- Plant Molecular Biology, Vol. 60, Issue 2, p. 225-239
Rice Brittle culm 6 encodes a dominant-negative form of CesA protein that perturbs cellulose synthesis in secondary cell walls
journal, January 2011
- Kotake, Toshihisa; Aohara, Tsutomu; Hirano, Ko
- Journal of Experimental Botany, Vol. 62, Issue 6
BRITTLE CULM1, Which Encodes a COBRA-Like Protein, Affects the Mechanical Properties of Rice Plants
journal, August 2003
- Li, Yunhai; Qian, Qian; Zhou, Yihua
- The Plant Cell, Vol. 15, Issue 9
Effect of nuclear matrix attachment regions on transgene expression in tobacco plants.
journal, September 2001
- Nowak, W.; Gawłowska, M.; Jarmołowski, A.
- Acta Biochimica Polonica, Vol. 48, Issue 3
Pathway of p-Coumaric Acid Incorporation into Maize Lignin As Revealed by NMR
journal, October 1994
- Ralph, John; Hatfield, Ronald D.; Quideau, Stephane
- Journal of the American Chemical Society, Vol. 116, Issue 21, p. 9448-9456
Pathway of p-Coumaric Acid Incorporation into Maize Lignin As Revealed by NMR
journal, October 1994
- Ralph, John; Hatfield, Ronald D.; Quideau, Stephane
- Journal of the American Chemical Society, Vol. 116, Issue 21, p. 9448-9456
PlaNet: Combined Sequence and Expression Comparisons across Plant Networks Derived from Seven Species
journal, March 2011
- Mutwil, Marek; Klie, Sebastian; Tohge, Takayuki
- The Plant Cell, Vol. 23, Issue 3
Gene silencing mediated by promoter homology occurs at the level of transcription and results in meiotically heritable alterations in methylation and gene activity
journal, February 1996
- Park, Y. -D.; Papp, I.; Moscone, E. A.
- The Plant Journal, Vol. 9, Issue 2
Rice Brittle culm 6 encodes a dominant-negative form of CesA protein that perturbs cellulose synthesis in secondary cell walls
journal, January 2011
- Kotake, Toshihisa; Aohara, Tsutomu; Hirano, Ko
- Journal of Experimental Botany, Vol. 62, Issue 6
The Arabidopsis Cellulose Synthase Complex: A Proposed Hexamer of CESA Trimers in an Equimolar Stoichiometry
journal, December 2014
- Hill, Joseph L.; Hammudi, Mustafa B.; Tien, Ming
- The Plant Cell, Vol. 26, Issue 12
Monolignol Ferulate Transferase Introduces Chemically Labile Linkages into the Lignin Backbone
journal, April 2014
- Wilkerson, C. G.; Mansfield, S. D.; Lu, F.
- Science, Vol. 344, Issue 6179, p. 90-93
Location of cellulose and callose in pollen tubes and grains of Nicotiana tabacum
journal, August 1998
- Ferguson, C.; Teeri, T. T.; Siika-aho, M.
- Planta, Vol. 206, Issue 3
Engineering the Oryza sativa cell wall with rice NAC transcription factors regulating secondary wall formation
journal, January 2013
- Yoshida, Kouki; Sakamoto, Shingo; Kawai, Tetsushi
- Frontiers in Plant Science, Vol. 4
Regulation of secondary cell wall biosynthesis by poplar R2R3 MYB transcription factor PtrMYB152 in Arabidopsis
journal, May 2014
- Wang, Shucai; Li, Eryang; Porth, Ilga
- Scientific Reports, Vol. 4, Issue 1
High-efficiency Agrobacterium-mediated transformation of Brachypodium distachyon inbred line Bd21-3
journal, November 2007
- Vogel, John; Hill, Theresa
- Plant Cell Reports, Vol. 27, Issue 3
Cell Walls and the Developmental Anatomy of the Brachypodium distachyon Stem Internode
journal, November 2013
- Matos, Dominick A.; Whitney, Ian P.; Harrington, Michael J.
- PLoS ONE, Vol. 8, Issue 11
A role for the rice homeobox gene Oshox1 in provascular cell fate commitment
journal, September 2000
- Scarpella, Enrico; Rueb, Saskia; Boot, Kees J. M.
- Development, Vol. 127, Issue 17
Works referencing / citing this record:
Functional Analysis of Cellulose Synthase CesA4 and CesA6 Genes in Switchgrass (Panicum virgatum) by Overexpression and RNAi-Mediated Gene Silencing
journal, August 2018
- Mazarei, Mitra; Baxter, Holly L.; Li, Mi
- Frontiers in Plant Science, Vol. 9