Functionalized Buckyballs for Visualizing Microbial Species in Different States and Environments
Abstract
To date, in situ visualization of microbial density has remained an open problem. Here, functionalized buckyballs (e.g., C60-pyrrolidine tris acid) are shown to be a versatile platform that allows internalization within a microorganism without either adhering to the cell wall and cell membrane or binding to a matrix substrate such as soil. These molecular probes are validated via multi-scale imaging, to show association with microorganisms via fluorescence microscopy, positive cellular uptake via electron microscopy, and non-specific binding to the substrates through a combination of fluorescence and autoradiography imaging. In conclusion, we also demonstrate that cysteine-functionalized C60- pyrrolidine tris acid can differentiate live and dead microorganisms.
- Authors:
-
- Univ. of Nevada, Reno, NV (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Brookhaven National Lab. (BNL), Upton, NY (United States)
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1255532
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Scientific Reports
- Additional Journal Information:
- Journal Volume: 5; Journal ID: ISSN 2045-2322
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Cheng, Qingsu, Aravind, Ashwin, Buckley, Matthew, Gifford, Andrew, and Parvin, Bahram. Functionalized Buckyballs for Visualizing Microbial Species in Different States and Environments. United States: N. p., 2015.
Web. doi:10.1038/srep13685.
Cheng, Qingsu, Aravind, Ashwin, Buckley, Matthew, Gifford, Andrew, & Parvin, Bahram. Functionalized Buckyballs for Visualizing Microbial Species in Different States and Environments. United States. https://doi.org/10.1038/srep13685
Cheng, Qingsu, Aravind, Ashwin, Buckley, Matthew, Gifford, Andrew, and Parvin, Bahram. Tue .
"Functionalized Buckyballs for Visualizing Microbial Species in Different States and Environments". United States. https://doi.org/10.1038/srep13685. https://www.osti.gov/servlets/purl/1255532.
@article{osti_1255532,
title = {Functionalized Buckyballs for Visualizing Microbial Species in Different States and Environments},
author = {Cheng, Qingsu and Aravind, Ashwin and Buckley, Matthew and Gifford, Andrew and Parvin, Bahram},
abstractNote = {To date, in situ visualization of microbial density has remained an open problem. Here, functionalized buckyballs (e.g., C60-pyrrolidine tris acid) are shown to be a versatile platform that allows internalization within a microorganism without either adhering to the cell wall and cell membrane or binding to a matrix substrate such as soil. These molecular probes are validated via multi-scale imaging, to show association with microorganisms via fluorescence microscopy, positive cellular uptake via electron microscopy, and non-specific binding to the substrates through a combination of fluorescence and autoradiography imaging. In conclusion, we also demonstrate that cysteine-functionalized C60- pyrrolidine tris acid can differentiate live and dead microorganisms.},
doi = {10.1038/srep13685},
journal = {Scientific Reports},
number = ,
volume = 5,
place = {United States},
year = {Tue Sep 08 00:00:00 EDT 2015},
month = {Tue Sep 08 00:00:00 EDT 2015}
}
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Proteomic Analysis of Carbon Concentrating Chemolithotrophic Bacteria Serratia sp. for Sequestration of Carbon Dioxide
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Collateral effects of antibiotics on mammalian gut microbiomes
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Works referencing / citing this record:
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Buckyballs conjugated with nucleic acid sequences identifies microorganisms in live cell assays
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