The Stanford Automated Mounter: Pushing the limits of sample exchange at the SSRL macromolecular crystallography beamlines
Abstract
The Stanford Automated Mounter System, a system for mounting and dismounting cryo-cooled crystals, has been upgraded to increase the throughput of samples on the macromolecular crystallography beamlines at the Stanford Synchrotron Radiation Lightsource. This upgrade speeds up robot maneuvers, reduces the heating/drying cycles, pre-fetches samples and adds an air-knife to remove frost from the gripper arms. As a result, sample pin exchange during automated crystal quality screening now takes about 25 s, five times faster than before this upgrade.
- Authors:
-
- SLAC National Accelerator Lab., Menlo Park, CA (United States)
- Publication Date:
- Research Org.:
- SLAC National Accelerator Lab., Menlo Park, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1253923
- Report Number(s):
- SLAC-PUB-16477
Journal ID: ISSN 1600-5767; JACGAR; PII: S1600576716000649
- Grant/Contract Number:
- AC02-76SF00515
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Applied Crystallography (Online)
- Additional Journal Information:
- Journal Name: Journal of Applied Crystallography (Online); Journal Volume: 49; Journal Issue: 2; Journal ID: ISSN 1600-5767
- Publisher:
- International Union of Crystallography
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 43 PARTICLE ACCELERATORS; automated crystal mounting; high-throughput sample exchange; sample exchange robot; synchrotron robotics; protein crystallography; remote access; cryo-crystallography
Citation Formats
Russi, Silvia, Song, Jinhu, McPhillips, Scott E., and Cohen, Aina E. The Stanford Automated Mounter: Pushing the limits of sample exchange at the SSRL macromolecular crystallography beamlines. United States: N. p., 2016.
Web. doi:10.1107/S1600576716000649.
Russi, Silvia, Song, Jinhu, McPhillips, Scott E., & Cohen, Aina E. The Stanford Automated Mounter: Pushing the limits of sample exchange at the SSRL macromolecular crystallography beamlines. United States. https://doi.org/10.1107/S1600576716000649
Russi, Silvia, Song, Jinhu, McPhillips, Scott E., and Cohen, Aina E. Wed .
"The Stanford Automated Mounter: Pushing the limits of sample exchange at the SSRL macromolecular crystallography beamlines". United States. https://doi.org/10.1107/S1600576716000649. https://www.osti.gov/servlets/purl/1253923.
@article{osti_1253923,
title = {The Stanford Automated Mounter: Pushing the limits of sample exchange at the SSRL macromolecular crystallography beamlines},
author = {Russi, Silvia and Song, Jinhu and McPhillips, Scott E. and Cohen, Aina E.},
abstractNote = {The Stanford Automated Mounter System, a system for mounting and dismounting cryo-cooled crystals, has been upgraded to increase the throughput of samples on the macromolecular crystallography beamlines at the Stanford Synchrotron Radiation Lightsource. This upgrade speeds up robot maneuvers, reduces the heating/drying cycles, pre-fetches samples and adds an air-knife to remove frost from the gripper arms. As a result, sample pin exchange during automated crystal quality screening now takes about 25 s, five times faster than before this upgrade.},
doi = {10.1107/S1600576716000649},
journal = {Journal of Applied Crystallography (Online)},
number = 2,
volume = 49,
place = {United States},
year = {Wed Feb 24 00:00:00 EST 2016},
month = {Wed Feb 24 00:00:00 EST 2016}
}
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Works referenced in this record:
High-density grids for efficient data collection from multiple crystals
journal, January 2016
- Baxter, Elizabeth L.; Aguila, Laura; Alonso-Mori, Roberto
- Acta Crystallographica Section D Structural Biology, Vol. 72, Issue 1, p. 2-11
Beamline automation and remote access at NSRRC BL-13
journal, August 2008
- Chao, C. -H.; Chiang, C. -H.; Tseng, C. -C.
- Acta Crystallographica Section A Foundations of Crystallography, Vol. 64, Issue a1
Implementation and performance of SIBYLS: a dual endstation small-angle X-ray scattering and macromolecular crystallography beamline at the Advanced Light Source
journal, January 2013
- Classen, Scott; Hura, Greg L.; Holton, James M.
- Journal of Applied Crystallography, Vol. 46, Issue 1
An automated system to mount cryo-cooled protein crystals on a synchrotron beamline, using compact sample cassettes and a small-scale robot
journal, November 2002
- Cohen, Aina E.; Ellis, Paul J.; Miller, Mitchell D.
- Journal of Applied Crystallography, Vol. 35, Issue 6
Goniometer-based femtosecond crystallography with X-ray free electron lasers
journal, October 2014
- Cohen, Aina E.; Soltis, S. Michael; González, Ana
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 48, p. 17122-17127
MX1: a bending-magnet crystallography beamline serving both chemical and macromolecular crystallography communities at the Australian Synchrotron
journal, January 2015
- Cowieson, Nathan Philip; Aragao, David; Clift, Mark
- Journal of Synchrotron Radiation, Vol. 22, Issue 1
Canadian macromolecular crystallography facility: a suite of fully automated beamlines
journal, January 2012
- Grochulski, Pawel; Fodje, Michel; Labiuk, Shaunivan
- Journal of Structural and Functional Genomics, Vol. 13, Issue 2
High-throughput operation of sample-exchange robots with double tongs at the Photon Factory beamlines
journal, April 2008
- Hiraki, Masahiko; Watanabe, Shokei; pHonda, Nobuo
- Journal of Synchrotron Radiation, Vol. 15, Issue 3
Improvement of an automated protein crystal exchange system PAM for high-throughput data collection
journal, October 2013
- Hiraki, Masahiko; Yamada, Yusuke; Chavas, Leonard M. G.
- Journal of Synchrotron Radiation, Vol. 20, Issue 6
Capture and X-ray diffraction studies of protein microcrystals in a microfluidic trap array
journal, March 2015
- Lyubimov, Artem Y.; Murray, Thomas D.; Koehl, Antoine
- Acta Crystallographica Section D Biological Crystallography, Vol. 71, Issue 4
Automated technologies and novel techniques to accelerate protein crystallography for structural genomics
journal, February 2008
- Manjasetty, Babu A.; Turnbull, Andrew P.; Panjikar, Santosh
- PROTEOMICS, Vol. 8, Issue 4
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