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Title: PrimerDesign-M: A multiple-alignment based multiple-primer design tool for walking across variable genomes

Abstract

Analyses of entire viral genomes or mtDNA requires comprehensive design of many primers across their genomes. In addition, simultaneous optimization of several DNA primer design criteria may improve overall experimental efficiency and downstream bioinformatic processing. To achieve these goals, we developed PrimerDesign-M. It includes several options for multiple-primer design, allowing researchers to efficiently design walking primers that cover long DNA targets, such as entire HIV-1 genomes, and that optimizes primers simultaneously informed by genetic diversity in multiple alignments and experimental design constraints given by the user. PrimerDesign-M can also design primers that include DNA barcodes and minimize primer dimerization. PrimerDesign-M finds optimal primers for highly variable DNA targets and facilitates design flexibility by suggesting alternative designs to adapt to experimental conditions.

Authors:
 [1];  [1]
  1. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Publication Date:
Research Org.:
Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1234421
Report Number(s):
LA-UR-14-29590
Journal ID: ISSN 1367-4803
Grant/Contract Number:  
AC52-06NA25396
Resource Type:
Accepted Manuscript
Journal Name:
Bioinformatics
Additional Journal Information:
Journal Volume: 31; Journal Issue: 9; Journal ID: ISSN 1367-4803
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 97 MATHEMATICS AND COMPUTING

Citation Formats

Yoon, Hyejin, and Leitner, Thomas. PrimerDesign-M: A multiple-alignment based multiple-primer design tool for walking across variable genomes. United States: N. p., 2014. Web. doi:10.1093/bioinformatics/btu832.
Yoon, Hyejin, & Leitner, Thomas. PrimerDesign-M: A multiple-alignment based multiple-primer design tool for walking across variable genomes. United States. https://doi.org/10.1093/bioinformatics/btu832
Yoon, Hyejin, and Leitner, Thomas. Wed . "PrimerDesign-M: A multiple-alignment based multiple-primer design tool for walking across variable genomes". United States. https://doi.org/10.1093/bioinformatics/btu832. https://www.osti.gov/servlets/purl/1234421.
@article{osti_1234421,
title = {PrimerDesign-M: A multiple-alignment based multiple-primer design tool for walking across variable genomes},
author = {Yoon, Hyejin and Leitner, Thomas},
abstractNote = {Analyses of entire viral genomes or mtDNA requires comprehensive design of many primers across their genomes. In addition, simultaneous optimization of several DNA primer design criteria may improve overall experimental efficiency and downstream bioinformatic processing. To achieve these goals, we developed PrimerDesign-M. It includes several options for multiple-primer design, allowing researchers to efficiently design walking primers that cover long DNA targets, such as entire HIV-1 genomes, and that optimizes primers simultaneously informed by genetic diversity in multiple alignments and experimental design constraints given by the user. PrimerDesign-M can also design primers that include DNA barcodes and minimize primer dimerization. PrimerDesign-M finds optimal primers for highly variable DNA targets and facilitates design flexibility by suggesting alternative designs to adapt to experimental conditions.},
doi = {10.1093/bioinformatics/btu832},
journal = {Bioinformatics},
number = 9,
volume = 31,
place = {United States},
year = {Wed Dec 17 00:00:00 EST 2014},
month = {Wed Dec 17 00:00:00 EST 2014}
}

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Cited by: 32 works
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