Ternary structure reveals mechanism of a membrane diacylglycerol kinase
Abstract
Diacylglycerol kinase catalyses the ATP-dependent conversion of diacylglycerol to phosphatidic acid in the plasma membrane of Escherichia coli. The small size of this integral membrane trimer, which has 121 residues per subunit, means that available protein must be used economically to craft three catalytic and substrate-binding sites centred about the membrane/cytosol interface. How nature has accomplished this extraordinary feat is revealed here in a crystal structure of the kinase captured as a ternary complex with bound lipid substrate and an ATP analogue. Residues, identified as essential for activity by mutagenesis, decorate the active site and are rationalized by the ternary structure. The γ-phosphate of the ATP analogue is positioned for direct transfer to the primary hydroxyl of the lipid whose acyl chain is in the membrane. A catalytic mechanism for this unique enzyme is proposed. As a result, the active site architecture shows clear evidence of having arisen by convergent evolution.
- Authors:
-
more »
- Trinity College Dublin, Dublin (Ireland)
- Univ. of Oxford, Oxford (United Kingdom)
- Arizona State Univ., Tempe, AZ (United States)
- Univ. of Southern California, Los Angeles, CA (United States)
- Univ. College Dublin, Dublin (Ireland)
- Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
- Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Univ. of Hamburg, Hamburg (Germany)
- Univ. of Konstanz, Konstanz (Germany)
- SLAC National Accelerator Lab., Menlo Park, CA (United States)
- Publication Date:
- Research Org.:
- SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1233178
- Grant/Contract Number:
- AC03-76SF00515
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nature Communications
- Additional Journal Information:
- Journal Volume: 6; Journal Issue: 1; Journal ID: ISSN 2041-1723
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES; biological sciences; biochemistry; biophysics
Citation Formats
Li, Dianfan, Stansfeld, Phillip J., Sansom, Mark S. P., Keogh, Aaron, Vogeley, Lutz, Howe, Nicole, Lyons, Joseph A., Aragao, David, Fromme, Petra, Fromme, Raimund, Basu, Shibom, Grotjohann, Ingo, Kupitz, Christopher, Rendek, Kimberley, Weierstall, Uwe, Zatsepin, Nadia A., Cherezov, Vadim, Liu, Wei, Bandaru, Sateesh, English, Niall J., Gati, Cornelius, Barty, Anton, Yefanov, Oleksandr, Chapman, Henry N., Diederichs, Kay, Messerschmidt, Marc, Boutet, Sébastien, Williams, Garth J., Marvin Seibert, M., and Caffrey, Martin. Ternary structure reveals mechanism of a membrane diacylglycerol kinase. United States: N. p., 2015.
Web. doi:10.1038/ncomms10140.
Li, Dianfan, Stansfeld, Phillip J., Sansom, Mark S. P., Keogh, Aaron, Vogeley, Lutz, Howe, Nicole, Lyons, Joseph A., Aragao, David, Fromme, Petra, Fromme, Raimund, Basu, Shibom, Grotjohann, Ingo, Kupitz, Christopher, Rendek, Kimberley, Weierstall, Uwe, Zatsepin, Nadia A., Cherezov, Vadim, Liu, Wei, Bandaru, Sateesh, English, Niall J., Gati, Cornelius, Barty, Anton, Yefanov, Oleksandr, Chapman, Henry N., Diederichs, Kay, Messerschmidt, Marc, Boutet, Sébastien, Williams, Garth J., Marvin Seibert, M., & Caffrey, Martin. Ternary structure reveals mechanism of a membrane diacylglycerol kinase. United States. https://doi.org/10.1038/ncomms10140
Li, Dianfan, Stansfeld, Phillip J., Sansom, Mark S. P., Keogh, Aaron, Vogeley, Lutz, Howe, Nicole, Lyons, Joseph A., Aragao, David, Fromme, Petra, Fromme, Raimund, Basu, Shibom, Grotjohann, Ingo, Kupitz, Christopher, Rendek, Kimberley, Weierstall, Uwe, Zatsepin, Nadia A., Cherezov, Vadim, Liu, Wei, Bandaru, Sateesh, English, Niall J., Gati, Cornelius, Barty, Anton, Yefanov, Oleksandr, Chapman, Henry N., Diederichs, Kay, Messerschmidt, Marc, Boutet, Sébastien, Williams, Garth J., Marvin Seibert, M., and Caffrey, Martin. Thu .
"Ternary structure reveals mechanism of a membrane diacylglycerol kinase". United States. https://doi.org/10.1038/ncomms10140. https://www.osti.gov/servlets/purl/1233178.
@article{osti_1233178,
title = {Ternary structure reveals mechanism of a membrane diacylglycerol kinase},
author = {Li, Dianfan and Stansfeld, Phillip J. and Sansom, Mark S. P. and Keogh, Aaron and Vogeley, Lutz and Howe, Nicole and Lyons, Joseph A. and Aragao, David and Fromme, Petra and Fromme, Raimund and Basu, Shibom and Grotjohann, Ingo and Kupitz, Christopher and Rendek, Kimberley and Weierstall, Uwe and Zatsepin, Nadia A. and Cherezov, Vadim and Liu, Wei and Bandaru, Sateesh and English, Niall J. and Gati, Cornelius and Barty, Anton and Yefanov, Oleksandr and Chapman, Henry N. and Diederichs, Kay and Messerschmidt, Marc and Boutet, Sébastien and Williams, Garth J. and Marvin Seibert, M. and Caffrey, Martin},
abstractNote = {Diacylglycerol kinase catalyses the ATP-dependent conversion of diacylglycerol to phosphatidic acid in the plasma membrane of Escherichia coli. The small size of this integral membrane trimer, which has 121 residues per subunit, means that available protein must be used economically to craft three catalytic and substrate-binding sites centred about the membrane/cytosol interface. How nature has accomplished this extraordinary feat is revealed here in a crystal structure of the kinase captured as a ternary complex with bound lipid substrate and an ATP analogue. Residues, identified as essential for activity by mutagenesis, decorate the active site and are rationalized by the ternary structure. The γ-phosphate of the ATP analogue is positioned for direct transfer to the primary hydroxyl of the lipid whose acyl chain is in the membrane. A catalytic mechanism for this unique enzyme is proposed. As a result, the active site architecture shows clear evidence of having arisen by convergent evolution.},
doi = {10.1038/ncomms10140},
journal = {Nature Communications},
number = 1,
volume = 6,
place = {United States},
year = {Thu Dec 17 00:00:00 EST 2015},
month = {Thu Dec 17 00:00:00 EST 2015}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 22 works
Citation information provided by
Web of Science
Web of Science
Figures / Tables:
Table 1: Crystallization conditions, data collection and refinement statistics.
All figures and tables
(7 total)
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Biomolecular cryocrystallography: Structural changes during flash-cooling
journal, March 2004
- Halle, B.
- Proceedings of the National Academy of Sciences, Vol. 101, Issue 14
Active Sites of Diacylglycerol Kinase from Escherichia coli Are Shared between Subunits †
journal, April 1999
- Lau, Francis W.; Chen, Xing; Bowie, James U.
- Biochemistry, Vol. 38, Issue 17
Linking Crystallographic Model and Data Quality
journal, May 2012
- Karplus, P. A.; Diederichs, K.
- Science, Vol. 336, Issue 6084
CrystFEL : a software suite for snapshot serial crystallography
journal, March 2012
- White, Thomas A.; Kirian, Richard A.; Martin, Andrew V.
- Journal of Applied Crystallography, Vol. 45, Issue 2
The Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS)
journal, March 2010
- Boutet, Sébastien; J. Williams, Garth
- New Journal of Physics, Vol. 12, Issue 3
Active Sites of Diacylglycerol Kinase from Escherichia coli Are Shared between Subunits †
journal, April 1999
- Lau, Francis W.; Chen, Xing; Bowie, James U.
- Biochemistry, Vol. 38, Issue 17
PHENIX: a comprehensive Python-based system for macromolecular structure solution.
text, January 2010
- Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
- Apollo - University of Cambridge Repository
Irreversible Misfolding of Diacylglycerol Kinase Is Independent of Aggregation and Occurs Prior to Trimerization and Membrane Association †
journal, August 2006
- Mi, Dehui; Kim, Hak Jun; Hadziselimovic, Arina
- Biochemistry, Vol. 45, Issue 33
PHENIX: a comprehensive Python-based system for macromolecular structure solution.
text, January 2010
- Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
- Apollo - University of Cambridge Repository
A biomolecular force field based on the free enthalpy of hydration and solvation: The GROMOS force-field parameter sets 53A5 and 53A6
journal, January 2004
- Oostenbrink, Chris; Villa, Alessandra; Mark, Alan E.
- Journal of Computational Chemistry, Vol. 25, Issue 13
Escherichia coli Diacylglycerol Kinase Is an Evolutionarily Optimized Membrane Enzyme and Catalyzes Direct Phosphoryl Transfer
journal, September 1997
- Badola, Prakash; Sanders, Charles R.
- Journal of Biological Chemistry, Vol. 272, Issue 39
Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography
journal, September 2012
- Li, Dianfan; Boland, Coilín; Aragao, David
- Journal of Visualized Experiments, Issue 67
A biomolecular force field based on the free enthalpy of hydration and solvation: The GROMOS force-field parameter sets 53A5 and 53A6
journal, January 2004
- Oostenbrink, Chris; Villa, Alessandra; Mark, Alan E.
- Journal of Computational Chemistry, Vol. 25, Issue 13
Coot model-building tools for molecular graphics
journal, November 2004
- Emsley, Paul; Cowtan, Kevin
- Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132
Lipid cubic phase as a membrane mimetic for integral membrane protein enzymes
journal, May 2011
- Li, D.; Caffrey, M.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 21
Femtosecond X-ray protein nanocrystallography
journal, February 2011
- Chapman, Henry N.; Fromme, Petra; Barty, Anton
- Nature, Vol. 470, Issue 7332, p. 73-77
Protein structural ensembles are revealed by redefining X-ray electron density noise
journal, December 2013
- Lang, P. T.; Holton, J. M.; Fraser, J. S.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 1
Geometry optimization with QM/MM, ONIOM, and other combined methods. I. Microiterations and constraints
journal, April 2003
- Vreven, Thom; Morokuma, Keiji; Farkas, Ödön
- Journal of Computational Chemistry, Vol. 24, Issue 6
Building a Thermostable Membrane Protein
journal, March 2000
- Zhou, Yufeng; Bowie, James U.
- Journal of Biological Chemistry, Vol. 275, Issue 10
Exploring the allowed sequence space of a membrane protein
journal, February 1996
- Wen, Juan; Chen, Xing; Bowie, James U.
- Nature Structural Biology, Vol. 3, Issue 2
Decision making in xia 2
journal, June 2013
- Winter, Graeme; Lobley, Carina M. C.; Prince, Stephen M.
- Acta Crystallographica Section D Biological Crystallography, Vol. 69, Issue 7
GROMACS 4.5: a high-throughput and highly parallel open source molecular simulation toolkit
journal, February 2013
- Pronk, Sander; Páll, Szilárd; Schulz, Roland
- Bioinformatics, Vol. 29, Issue 7
From Coarse Grained to Atomistic: A Serial Multiscale Approach to Membrane Protein Simulations
journal, March 2011
- Stansfeld, Phillip J.; Sansom, Mark S. P.
- Journal of Chemical Theory and Computation, Vol. 7, Issue 4
Geometry optimization with QM/MM, ONIOM, and other combined methods. I. Microiterations and constraints
journal, April 2003
- Vreven, Thom; Morokuma, Keiji; Farkas, Ödön
- Journal of Computational Chemistry, Vol. 24, Issue 6
Changing single side-chains can greatly enhance the resistance of a membrane protein to irreversible inactivation
journal, July 1999
- Lau, Francis W.; Nauli, Sehat; Zhou, Yufeng
- Journal of Molecular Biology, Vol. 290, Issue 2
Membrane topology of Escherichia coli diacylglycerol kinase.
journal, September 1994
- Smith, R. L.; O'Toole, J. F.; Maguire, M. E.
- Journal of Bacteriology, Vol. 176, Issue 17
Quantum and Classical Dynamics Simulations of ATP Hydrolysis in Solution
journal, June 2012
- Harrison, Christopher B.; Schulten, Klaus
- Journal of Chemical Theory and Computation, Vol. 8, Issue 7
Membrane topology of Escherichia coli diacylglycerol kinase.
journal, September 1994
- Smith, R. L.; O'Toole, J. F.; Maguire, M. E.
- Journal of Bacteriology, Vol. 176, Issue 17
Escherichia coli Diacylglycerol Kinase Is an α-Helical Polytopic Membrane Protein and Can Spontaneously Insert into Preformed Lipid Vesicles †
journal, January 1996
- Sanders, Charles R.; Czerski, Lech; Vinogradova, Olga
- Biochemistry, Vol. 35, Issue 26
FEM: feature-enhanced map
journal, February 2015
- Afonine, Pavel V.; Moriarty, Nigel W.; Mustyakimov, Marat
- Acta Crystallographica Section D Biological Crystallography, Vol. 71, Issue 3
Multiscale Simulations Reveal Conserved Patterns of Lipid Interactions with Aquaporins
journal, May 2013
- Stansfeld, Phillip J.; Jefferys, Elizabeth E.; Sansom, Mark S. P.
- Structure, Vol. 21, Issue 5
X-ray lasers for structural and dynamic biology
journal, September 2012
- Spence, J. C. H.; Weierstall, U.; Chapman, H. N.
- Reports on Progress in Physics, Vol. 75, Issue 10
The Structural Basis for Control of Eukaryotic Protein Kinases
journal, July 2012
- Endicott, Jane A.; Noble, Martin E. M.; Johnson, Louise N.
- Annual Review of Biochemistry, Vol. 81, Issue 1
Mapping the Oligomeric Interface of Diacylglycerol Kinase by Engineered Thiol Cross-Linking: Homologous Sites in the Transmembrane Domain †
journal, April 2000
- Nagy, Joanna K.; Lau, Francis W.; Bowie, James U.
- Biochemistry, Vol. 39, Issue 14
Serial Femtosecond Crystallography of G Protein-Coupled Receptors
journal, December 2013
- Liu, W.; Wacker, D.; Gati, C.
- Science, Vol. 342, Issue 6165
2.2 Å refined crystal structure of the catalytic subunit of cAMP-dependent protein kinase complexed with MnATP and a peptide inhibitor
journal, May 1993
- Zheng, J.; Trafny, E. A.; Knighton, D. R.
- Acta Crystallographica Section D Biological Crystallography, Vol. 49, Issue 3
Renaturing Membrane Proteins in the Lipid Cubic Phase, a Nanoporous Membrane Mimetic
journal, July 2014
- Li, Dianfan; Caffrey, Martin
- Scientific Reports, Vol. 4, Issue 1
Crystal structure of the integral membrane diacylglycerol kinase
journal, May 2013
- Li, Dianfan; Lyons, Joseph A.; Pye, Valerie E.
- Nature, Vol. 497, Issue 7450
Cavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation
journal, February 2021
- Tillu, Vikas A.; Rae, James; Gao, Ya
- Nature Communications, Vol. 12, Issue 1
Renaturing Membrane Proteins in the Lipid Cubic Phase, a Nanoporous Membrane Mimetic
journal, July 2014
- Li, Dianfan; Caffrey, Martin
- Scientific Reports, Vol. 4, Issue 1
Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography
journal, February 2014
- Weierstall, Uwe; James, Daniel; Wang, Chong
- Nature Communications, Vol. 5, Issue 1
Diffraction before destruction
journal, July 2014
- Chapman, Henry N.; Caleman, Carl; Timneanu, Nicusor
- Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 369, Issue 1647
Prokaryotic Diacylglycerol Kinase and Undecaprenol Kinase
journal, June 2012
- Van Horn, Wade D.; Sanders, Charles R.
- Annual Review of Biophysics, Vol. 41, Issue 1
Host Lipid and Temperature as Important Screening Variables for Crystallizing Integral Membrane Proteins in Lipidic Mesophases. Trials with Diacylglycerol Kinase
journal, June 2013
- Li, Dianfan; Shah, Syed T. A.; Caffrey, Martin
- Crystal Growth & Design, Vol. 13, Issue 7
Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography
journal, February 2014
- Weierstall, Uwe; James, Daniel; Wang, Chong
- Nature Communications, Vol. 5, Issue 1
‘Hit and run’ serial femtosecond crystallography of a membrane kinase in the lipid cubic phase
journal, July 2014
- Caffrey, Martin; Li, Dianfan; Howe, Nicole
- Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 369, Issue 1647
CSPAD-140k: A versatile detector for LCLS experiments
journal, August 2013
- Herrmann, Sven; Boutet, Sébastien; Duda, Brian
- Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Vol. 718
Quantum and Classical Dynamics Simulations of ATP Hydrolysis in Solution
journal, June 2012
- Harrison, Christopher B.; Schulten, Klaus
- Journal of Chemical Theory and Computation, Vol. 8, Issue 7
Mapping the Oligomeric Interface of Diacylglycerol Kinase by Engineered Thiol Cross-Linking: Homologous Sites in the Transmembrane Domain †
journal, April 2000
- Nagy, Joanna K.; Lau, Francis W.; Bowie, James U.
- Biochemistry, Vol. 39, Issue 14
Solution Nuclear Magnetic Resonance Structure of Membrane-Integral Diacylglycerol Kinase
journal, June 2009
- Van Horn, W. D.; Kim, H. -J.; Ellis, C. D.
- Science, Vol. 324, Issue 5935
PHENIX: a comprehensive Python-based system for macromolecular structure solution
journal, January 2010
- Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
- Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221
Building a Thermostable Membrane Protein
journal, March 2000
- Zhou, Yufeng; Bowie, James U.
- Journal of Biological Chemistry, Vol. 275, Issue 10
Structural changes in a cryo-cooled protein crystal owing to radiation damage
journal, March 2000
- Burmeister, Wilhelm Pascal
- Acta Crystallographica Section D Biological Crystallography, Vol. 56, Issue 3
Exploring the allowed sequence space of a membrane protein
journal, February 1996
- Wen, Juan; Chen, Xing; Bowie, James U.
- Nature Structural Biology, Vol. 3, Issue 2
Cheetah : software for high-throughput reduction and analysis of serial femtosecond X-ray diffraction data
journal, May 2014
- Barty, Anton; Kirian, Richard A.; Maia, Filipe R. N. C.
- Journal of Applied Crystallography, Vol. 47, Issue 3
Escherichia coli Diacylglycerol Kinase Is an α-Helical Polytopic Membrane Protein and Can Spontaneously Insert into Preformed Lipid Vesicles †
journal, January 1996
- Sanders, Charles R.; Czerski, Lech; Vinogradova, Olga
- Biochemistry, Vol. 35, Issue 26
Preparation of microcrystals in lipidic cubic phase for serial femtosecond crystallography
journal, August 2014
- Liu, Wei; Ishchenko, Andrii; Cherezov, Vadim
- Nature Protocols, Vol. 9, Issue 9
Protein structural ensembles are revealed by redefining X-ray electron density noise
journal, December 2013
- Lang, P. T.; Holton, J. M.; Fraser, J. S.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 1
Preparation of microcrystals in lipidic cubic phase for serial femtosecond crystallography
journal, August 2014
- Liu, Wei; Ishchenko, Andrii; Cherezov, Vadim
- Nature Protocols, Vol. 9, Issue 9
From Coarse Grained to Atomistic: A Serial Multiscale Approach to Membrane Protein Simulations
journal, March 2011
- Stansfeld, Phillip J.; Sansom, Mark S. P.
- Journal of Chemical Theory and Computation, Vol. 7, Issue 4
X-ray lasers for structural and dynamic biology
journal, September 2012
- Spence, J. C. H.; Weierstall, U.; Chapman, H. N.
- Reports on Progress in Physics, Vol. 75, Issue 10
GROMACS 4.5: a high-throughput and highly parallel open source molecular simulation toolkit
journal, February 2013
- Pronk, Sander; Páll, Szilárd; Schulz, Roland
- Bioinformatics, Vol. 29, Issue 7
The Structural Basis for Control of Eukaryotic Protein Kinases
journal, July 2012
- Endicott, Jane A.; Noble, Martin E. M.; Johnson, Louise N.
- Annual Review of Biochemistry, Vol. 81, Issue 1
‘Hit and run’ serial femtosecond crystallography of a membrane kinase in the lipid cubic phase
journal, July 2014
- Caffrey, Martin; Li, Dianfan; Howe, Nicole
- Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 369, Issue 1647
Crystal structure of the integral membrane diacylglycerol kinase
journal, May 2013
- Li, Dianfan; Lyons, Joseph A.; Pye, Valerie E.
- Nature, Vol. 497, Issue 7450
Prokaryotic Diacylglycerol Kinase and Undecaprenol Kinase
journal, June 2012
- Van Horn, Wade D.; Sanders, Charles R.
- Annual Review of Biophysics, Vol. 41, Issue 1
Escherichia coli Diacylglycerol Kinase Is an Evolutionarily Optimized Membrane Enzyme and Catalyzes Direct Phosphoryl Transfer
journal, September 1997
- Badola, Prakash; Sanders, Charles R.
- Journal of Biological Chemistry, Vol. 272, Issue 39
Cell-free expression and in meso crystallisation of an integral membrane kinase for structure determination
journal, July 2014
- Boland, Coilín; Li, Dianfan; Shah, Syed Tasadaque Ali
- Cellular and Molecular Life Sciences, Vol. 71, Issue 24
Diffraction before destruction
journal, July 2014
- Chapman, Henry N.; Caleman, Carl; Timneanu, Nicusor
- Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 369, Issue 1647
Accessing protein conformational ensembles using room-temperature X-ray crystallography
journal, September 2011
- Fraser, J. S.; van den Bedem, H.; Samelson, A. J.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 39
Lipid cubic phase as a membrane mimetic for integral membrane protein enzymes
journal, May 2011
- Li, D.; Caffrey, M.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 21
Phaser crystallographic software
journal, July 2007
- McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D.
- Journal of Applied Crystallography, Vol. 40, Issue 4
Host Lipid and Temperature as Important Screening Variables for Crystallizing Integral Membrane Proteins in Lipidic Mesophases. Trials with Diacylglycerol Kinase
journal, June 2013
- Li, Dianfan; Shah, Syed T. A.; Caffrey, Martin
- Crystal Growth & Design, Vol. 13, Issue 7
Changing single side-chains can greatly enhance the resistance of a membrane protein to irreversible inactivation
journal, July 1999
- Lau, Francis W.; Nauli, Sehat; Zhou, Yufeng
- Journal of Molecular Biology, Vol. 290, Issue 2
sn-1,2-Diacylglycerol kinase of Escherichia coli. Mixed micellar analysis of the phospholipid cofactor requirement and divalent cation dependence.
journal, May 1986
- Walsh, J. P.; Bell, R. M.
- Journal of Biological Chemistry, Vol. 261, Issue 14
Works referencing / citing this record:
In meso in situ serial X-ray crystallography of soluble and membrane proteins at cryogenic temperatures
journal, January 2016
- Huang, Chia-Ying; Olieric, Vincent; Ma, Pikyee
- Acta Crystallographica Section D Structural Biology, Vol. 72, Issue 1
Global response of diacylglycerol kinase towards substrate binding observed by 2D and 3D MAS NMR
text, January 2019
- Möbius, Kristin; Kazemi, Sina; Güntert, Peter
- ETH Zurich
Global response of diacylglycerol kinase towards substrate binding observed by 2D and 3D MAS NMR
journal, March 2019
- Möbius, Kristin; Kazemi, Sina; Güntert, Peter
- Scientific Reports, Vol. 9, Issue 1
Folding and Misfolding of Human Membrane Proteins in Health and Disease: From Single Molecules to Cellular Proteostasis
journal, January 2019
- Marinko, Justin T.; Huang, Hui; Penn, Wesley D.
- Chemical Reviews, Vol. 119, Issue 9
Crystal structure of undecaprenyl-pyrophosphate phosphatase and its role in peptidoglycan biosynthesis
journal, March 2018
- El Ghachi, Meriem; Howe, Nicole; Huang, Chia-Ying
- Nature Communications, Vol. 9, Issue 1
Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography
text, January 2016
- Nogly, Przemyslaw; Panneels, Valerie; Nelson, Garrett
- Deutsches Elektronen-Synchrotron, DESY, Hamburg
Crystal structure of undecaprenyl-pyrophosphate phosphatase and its role in peptidoglycan biosynthesis
journal, March 2018
- El Ghachi, Meriem; Howe, Nicole; Huang, Chia-Ying
- Nature Communications, Vol. 9, Issue 1
Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography
text, January 2016
- Nogly, Przemyslaw; Panneels, Valérie; Nelson, Garrett
- ETH Zurich
Global response of diacylglycerol kinase towards substrate binding observed by 2D and 3D MAS NMR
text, January 2019
- Möbius, Kristin; Kazemi, Sina; Güntert, Peter
- ETH Zurich
Figures / Tables found in this record:
Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.