Multi-matrix, dual polarity, tandem mass spectrometry imaging strategy applied to a germinated maize seed: toward mass spectrometry imaging of an untargeted metabolome
Abstract
Mass spectrometry imaging (MSI) provides high spatial resolution information that is unprecedented in traditional metabolomics analyses; however, the molecular coverage is often limited to a handful of compounds and is insufficient to understand overall metabolomic changes of a biological system. Here, we propose an MSI methodology to increase the diversity of chemical compounds that can be imaged and identified, in order to eventually perform untargeted metabolomic analysis using MSI. We use the desorption/ionization bias of various matrixes for different metabolite classes along with dual polarities and a tandem MSI strategy. The use of multiple matrixes and dual polarities allows us to visualize various classes of compounds, while data-dependent MS/MS spectra acquired in the same MSI scans allow us to identify the compounds directly on the tissue. In a proof of concept application to a germinated corn seed, a total of 166 unique ions were determined to have high-quality MS/MS spectra, without counting structural isomers, of which 52 were identified as unique compounds. According to an estimation based on precursor MSI datasets, we expect over five hundred metabolites could be potentially identified and visualized once all experimental conditions are optimized and an MS/MS library is available. Finally, metabolites involved in themore »
- Authors:
-
- Iowa State Univ., Ames, IA (United States); Ames Lab., Ames, IA (United States)
- Publication Date:
- Research Org.:
- Ames Laboratory (AMES), Ames, IA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1227419
- Report Number(s):
- IS-J 8829
Journal ID: ISSN 0003-2654; ANALAO
- Grant/Contract Number:
- AC02-07CH11358
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Analyst
- Additional Journal Information:
- Journal Volume: 140; Journal Issue: 21; Journal ID: ISSN 0003-2654
- Publisher:
- Royal Society of Chemistry
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Feenstra, Adam D., Hansen, Rebecca L., and Lee, Young Jin. Multi-matrix, dual polarity, tandem mass spectrometry imaging strategy applied to a germinated maize seed: toward mass spectrometry imaging of an untargeted metabolome. United States: N. p., 2015.
Web. doi:10.1039/c5an01079a.
Feenstra, Adam D., Hansen, Rebecca L., & Lee, Young Jin. Multi-matrix, dual polarity, tandem mass spectrometry imaging strategy applied to a germinated maize seed: toward mass spectrometry imaging of an untargeted metabolome. United States. https://doi.org/10.1039/c5an01079a
Feenstra, Adam D., Hansen, Rebecca L., and Lee, Young Jin. Thu .
"Multi-matrix, dual polarity, tandem mass spectrometry imaging strategy applied to a germinated maize seed: toward mass spectrometry imaging of an untargeted metabolome". United States. https://doi.org/10.1039/c5an01079a. https://www.osti.gov/servlets/purl/1227419.
@article{osti_1227419,
title = {Multi-matrix, dual polarity, tandem mass spectrometry imaging strategy applied to a germinated maize seed: toward mass spectrometry imaging of an untargeted metabolome},
author = {Feenstra, Adam D. and Hansen, Rebecca L. and Lee, Young Jin},
abstractNote = {Mass spectrometry imaging (MSI) provides high spatial resolution information that is unprecedented in traditional metabolomics analyses; however, the molecular coverage is often limited to a handful of compounds and is insufficient to understand overall metabolomic changes of a biological system. Here, we propose an MSI methodology to increase the diversity of chemical compounds that can be imaged and identified, in order to eventually perform untargeted metabolomic analysis using MSI. We use the desorption/ionization bias of various matrixes for different metabolite classes along with dual polarities and a tandem MSI strategy. The use of multiple matrixes and dual polarities allows us to visualize various classes of compounds, while data-dependent MS/MS spectra acquired in the same MSI scans allow us to identify the compounds directly on the tissue. In a proof of concept application to a germinated corn seed, a total of 166 unique ions were determined to have high-quality MS/MS spectra, without counting structural isomers, of which 52 were identified as unique compounds. According to an estimation based on precursor MSI datasets, we expect over five hundred metabolites could be potentially identified and visualized once all experimental conditions are optimized and an MS/MS library is available. Finally, metabolites involved in the glycolysis pathway and tricarboxylic acid cycle were imaged to demonstrate the potential of this technology to better understand metabolic biology.},
doi = {10.1039/c5an01079a},
journal = {Analyst},
number = 21,
volume = 140,
place = {United States},
year = {Thu Aug 27 00:00:00 EDT 2015},
month = {Thu Aug 27 00:00:00 EDT 2015}
}
Web of Science
Works referenced in this record:
Molecular Imaging of Biological Samples: Localization of Peptides and Proteins Using MALDI-TOF MS
journal, December 1997
- Caprioli, Richard M.; Farmer, Terry B.; Gile, Jocelyn
- Analytical Chemistry, Vol. 69, Issue 23
Mass spectrometric imaging of small molecules
journal, August 2010
- Svatoš, Aleš
- Trends in Biotechnology, Vol. 28, Issue 8
Imaging mass spectrometry: From tissue sections to cell cultures
journal, July 2013
- Weaver, Eric M.; Hummon, Amanda B.
- Advanced Drug Delivery Reviews, Vol. 65, Issue 8
Imaging mass spectrometry
journal, January 2007
- McDonnell, Liam A.; Heeren, Ron M. A.
- Mass Spectrometry Reviews, Vol. 26, Issue 4
A concise review of mass spectrometry imaging
journal, June 2010
- Amstalden van Hove, Erika R.; Smith, Donald F.; Heeren, Ron M. A.
- Journal of Chromatography A, Vol. 1217, Issue 25
Drug localization in different lung cancer phenotypes by MALDI mass spectrometry imaging
journal, June 2011
- Marko-Varga, György; Fehniger, Thomas E.; Rezeli, Melinda
- Journal of Proteomics, Vol. 74, Issue 7
MALDI mass spectrometry-assisted molecular imaging of metabolites during nitrogen fixation in the Medicago truncatula - Sinorhizobium meliloti symbiosis
journal, May 2013
- Ye, Hui; Gemperline, Erin; Venkateshwaran, Muthusubramanian
- The Plant Journal, Vol. 75, Issue 1
Phytochemistry meets genome analysis, and beyond.........
journal, March 2003
- Dixon, Richard A.; Strack, Dieter
- Phytochemistry, Vol. 62, Issue 6
Metabolomics by numbers: acquiring and understanding global metabolite data
journal, May 2004
- Goodacre, Royston; Vaidyanathan, Seetharaman; Dunn, Warwick B.
- Trends in Biotechnology, Vol. 22, Issue 5
Metabolite Profiling of Germinating Rice Seeds
journal, December 2008
- Shu, Xiao-Li; Frank, Thomas; Shu, Qing-Yao
- Journal of Agricultural and Food Chemistry, Vol. 56, Issue 24
Simultaneous analysis of metabolites in potato tuber by gas chromatography-mass spectrometry
journal, July 2000
- Roessner, Ute; Wagner, Cornelia; Kopka, Joachim
- The Plant Journal, Vol. 23, Issue 1
A Novel Approach for Nontargeted Data Analysis for Metabolomics. Large-Scale Profiling of Tomato Fruit Volatiles
journal, November 2005
- Tikunov, Yury; Lommen, Arjen; de Vos, C. H. Ric
- Plant Physiology, Vol. 139, Issue 3
Imaging MS Methodology for More Chemical Information in Less Data Acquisition Time Utilizing a Hybrid Linear Ion Trap−Orbitrap Mass Spectrometer
journal, November 2010
- Perdian, D. C.; Lee, Young Jin
- Analytical Chemistry, Vol. 82, Issue 22
Multiplex Mass Spectrometric Imaging with Polarity Switching for Concurrent Acquisition of Positive and Negative Ion Images
journal, April 2013
- Korte, Andrew R.; Lee, Young Jin
- Journal of The American Society for Mass Spectrometry, Vol. 24, Issue 6
Sublimation as a method of matrix application for mass spectrometric imaging
journal, September 2007
- Hankin, Joseph A.; Barkley, Robert M.; Murphy, Robert C.
- Journal of the American Society for Mass Spectrometry, Vol. 18, Issue 9
High-Spatial and High-Mass Resolution Imaging of Surface Metabolites of Arabidopsis thaliana by Laser Desorption-Ionization Mass Spectrometry Using Colloidal Silver
journal, April 2010
- Jun, Ji Hyun; Song, Zhihong; Liu, Zhenjiu
- Analytical Chemistry, Vol. 82, Issue 8
MSiReader: An Open-Source Interface to View and Analyze High Resolving Power MS Imaging Files on Matlab Platform
journal, March 2013
- Robichaud, Guillaume; Garrard, Kenneth P.; Barry, Jeremy A.
- Journal of The American Society for Mass Spectrometry, Vol. 24, Issue 5
Sublimation of New Matrix Candidates for High Spatial Resolution Imaging Mass Spectrometry of Lipids: Enhanced Information in Both Positive and Negative Polarities after 1,5-Diaminonapthalene Deposition
journal, January 2012
- Thomas, Aurélien; Charbonneau, Jade Laveaux; Fournaise, Erik
- Analytical Chemistry, Vol. 84, Issue 4
MALDI-MS analysis and imaging of small molecule metabolites with 1,5-diaminonaphthalene (DAN): Diaminonaphthalene as small molecule MALDI matrix
journal, July 2014
- Korte, Andrew R.; Lee, Young Jin
- Journal of Mass Spectrometry, Vol. 49, Issue 8
9-Aminoacridine as a matrix for negative mode matrix-assisted laser desorption/ionization
journal, January 2002
- Vermillion-Salsbury, Rachal L.; Hercules, David M.
- Rapid Communications in Mass Spectrometry, Vol. 16, Issue 16
Imaging Matrix Assisted Laser Desorption Ionization Mass Spectrometry: a technique to map plant metabolites within tissues at high spatial resolution
journal, September 2006
- Burrell, Mm; Earnshaw, Cj; Clench, Mr
- Journal of Experimental Botany, Vol. 58, Issue 4
In Situ Primary Metabolites Localization on a Rat Brain Section by Chemical Mass Spectrometry Imaging
journal, July 2009
- Benabdellah, Farida; Touboul, David; Brunelle, Alain
- Analytical Chemistry, Vol. 81, Issue 13
Imaging of lipids in rat heart by MALDI-MS with silver nanoparticles
journal, December 2013
- Jackson, Shelley N.; Baldwin, Kathrine; Muller, Ludovic
- Analytical and Bioanalytical Chemistry, Vol. 406, Issue 5
Imaging mass spectrometry with silver naeoparticles reveals the distribution of fatty acids in mouse retinal sections
journal, August 2010
- Hayasaka, Takahiro; Goto-Inoue, Naoko; Zaima, Nobuhiro
- Journal of the American Society for Mass Spectrometry, Vol. 21, Issue 8
Lipid Analysis by Matrix-Assisted Laser Desorption and Ionization Mass Spectrometry: A Methodological Approach
journal, February 1999
- Schiller, J.; Arnhold, J.; Benard, S.
- Analytical Biochemistry, Vol. 267, Issue 1
2,5-Dihydroxybenzoic acid: a new matrix for laser desorption—ionization mass spectrometry
journal, December 1991
- Strupat, K.; Karas, M.; Hillenkamp, F.
- International Journal of Mass Spectrometry and Ion Processes, Vol. 111
Quantitative aspects of the matrix-assisted laser desorption mass spectrometry of complex oligosaccharides
journal, July 1993
- Harvey, D. J.
- Rapid Communications in Mass Spectrometry, Vol. 7, Issue 7
The Quadrupole Ion Trap Mass Spectrometer—A Small Solution to a Big Challenge
journal, January 1997
- Jonscher, Karen R.; Yates, John R.
- Analytical Biochemistry, Vol. 244, Issue 1
In situ structural characterization of phosphatidylcholines in brain tissue using MALDI-MS/MS
journal, December 2005
- Jackson, Shelley N.; Wang, Hay-Yan J.; Woods, Amina S.
- Journal of the American Society for Mass Spectrometry, Vol. 16, Issue 12
Desorption electrospray ionization (DESI) mass spectrometry and tandem mass spectrometry (MS/MS) of phospholipids and sphingolipids: Ionization, adduct formation, and fragmentation
journal, April 2008
- Manicke, Nicholas E.; Wiseman, Justin M.; Ifa, Demian R.
- Journal of the American Society for Mass Spectrometry, Vol. 19, Issue 4
Fumonisin Disruption of Ceramide Biosynthesis in Maize Roots and the Effects on Plant Development and Fusarium verticillioides -Induced Seedling Disease
journal, April 2007
- Williams, Lonnie D.; Glenn, Anthony E.; Zimeri, Anne Marie
- Journal of Agricultural and Food Chemistry, Vol. 55, Issue 8
Characterization of Sphingolipids from Sunflower Seeds with Altered Fatty Acid Composition
journal, December 2011
- Salas, Joaquín J.; Markham, Jonathan E.; Martínez-Force, Enrique
- Journal of Agricultural and Food Chemistry, Vol. 59, Issue 23
The Organization and Regulation of Plant Glycolysis
journal, June 1996
- Plaxton, William C.
- Annual Review of Plant Physiology and Plant Molecular Biology, Vol. 47, Issue 1
Works referencing / citing this record:
Organic–inorganic binary mixture matrix for comprehensive laser-desorption ionization mass spectrometric analysis and imaging of medium-size molecules including phospholipids, glycerolipids, and oligosaccharides
journal, January 2016
- Feenstra, Adam D.; O'Neill, Kelly C.; Yagnik, Gargey B.
- RSC Advances, Vol. 6, Issue 101
Recent advances in single-cell analysis by mass spectrometry
journal, January 2019
- Yin, Lei; Zhang, Zhi; Liu, Yingze
- The Analyst, Vol. 144, Issue 3
Mass Spectrometry Imaging of Complex Microbial Communities
journal, December 2016
- Dunham, Sage J. B.; Ellis, Joseph F.; Li, Bin
- Accounts of Chemical Research, Vol. 50, Issue 1
Toward Mass Spectrometry Imaging in the Metabolomics Scale: Increasing Metabolic Coverage Through Multiple On-Tissue Chemical Modifications
journal, July 2019
- Dueñas, Maria Emilia; Larson, Evan A.; Lee, Young Jin
- Frontiers in Plant Science, Vol. 10