A solvent replenishment solution for managing evaporation of biochemical reactions in air-matrix digital microfluidics devices
Abstract
Digital microfluidics (DMF) is a powerful technique for sample preparation and analysis for a broad range of biological and chemical applications. In many cases, it is desirable to carry out DMF on an open surface, such that the matrix surrounding the droplets is ambient air. However, the utility of the air-matrix DMF format has been severely limited by problems with droplet evaporation, especially when the droplet-based biochemical reactions require high temperatures for long periods of time. We present a simple solution for managing evaporation in air-matrix DMF: just-in-time replenishment of the reaction volume using droplets of solvent. We demonstrate that this solution enables DMF-mediated execution of several different biochemical reactions (RNA fragmentation, first-strand cDNA synthesis, and PCR) over a range of temperatures (4–95 °C) and incubation times (up to 1 h or more) without use of oil, humidifying chambers, or off-chip heating modules. Reaction volumes and temperatures were maintained roughly constant over the course of each experiment, such that the reaction kinetics and products generated by the air-matrix DMF device were comparable to those of conventional benchscale reactions. As a result, this simple yet effective solution for evaporation management is an important advance in developing air-matrix DMF for a widemore »
- Authors:
-
- Sandia National Laboratories (SNL-CA), Livermore, CA (United States)
- Publication Date:
- Research Org.:
- Sandia National Lab. (SNL-CA), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE National Nuclear Security Administration (NNSA)
- OSTI Identifier:
- 1225865
- Report Number(s):
- SAND-2015-0066J
Journal ID: ISSN 1473-0197; LCAHAM; 558325
- Grant/Contract Number:
- AC04-94AL85000
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Lab on a chip (Print)
- Additional Journal Information:
- Journal Name: Lab on a chip (Print); Journal Volume: 15; Journal Issue: 1; Journal ID: ISSN 1473-0197
- Publisher:
- Royal Society of Chemistry
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Jebrail, Mais J., Renzi, Ronald F., Sinha, Anupama, Van De Vreugde, Jim, Gondhalekar, Carmen, Ambriz, Cesar, Meagher, Robert J., and Branda, Steven S. A solvent replenishment solution for managing evaporation of biochemical reactions in air-matrix digital microfluidics devices. United States: N. p., 2014.
Web. doi:10.1039/c4lc00703d.
Jebrail, Mais J., Renzi, Ronald F., Sinha, Anupama, Van De Vreugde, Jim, Gondhalekar, Carmen, Ambriz, Cesar, Meagher, Robert J., & Branda, Steven S. A solvent replenishment solution for managing evaporation of biochemical reactions in air-matrix digital microfluidics devices. United States. https://doi.org/10.1039/c4lc00703d
Jebrail, Mais J., Renzi, Ronald F., Sinha, Anupama, Van De Vreugde, Jim, Gondhalekar, Carmen, Ambriz, Cesar, Meagher, Robert J., and Branda, Steven S. Wed .
"A solvent replenishment solution for managing evaporation of biochemical reactions in air-matrix digital microfluidics devices". United States. https://doi.org/10.1039/c4lc00703d. https://www.osti.gov/servlets/purl/1225865.
@article{osti_1225865,
title = {A solvent replenishment solution for managing evaporation of biochemical reactions in air-matrix digital microfluidics devices},
author = {Jebrail, Mais J. and Renzi, Ronald F. and Sinha, Anupama and Van De Vreugde, Jim and Gondhalekar, Carmen and Ambriz, Cesar and Meagher, Robert J. and Branda, Steven S.},
abstractNote = {Digital microfluidics (DMF) is a powerful technique for sample preparation and analysis for a broad range of biological and chemical applications. In many cases, it is desirable to carry out DMF on an open surface, such that the matrix surrounding the droplets is ambient air. However, the utility of the air-matrix DMF format has been severely limited by problems with droplet evaporation, especially when the droplet-based biochemical reactions require high temperatures for long periods of time. We present a simple solution for managing evaporation in air-matrix DMF: just-in-time replenishment of the reaction volume using droplets of solvent. We demonstrate that this solution enables DMF-mediated execution of several different biochemical reactions (RNA fragmentation, first-strand cDNA synthesis, and PCR) over a range of temperatures (4–95 °C) and incubation times (up to 1 h or more) without use of oil, humidifying chambers, or off-chip heating modules. Reaction volumes and temperatures were maintained roughly constant over the course of each experiment, such that the reaction kinetics and products generated by the air-matrix DMF device were comparable to those of conventional benchscale reactions. As a result, this simple yet effective solution for evaporation management is an important advance in developing air-matrix DMF for a wide variety of new, high-impact applications, particularly in the biomedical sciences.},
doi = {10.1039/c4lc00703d},
journal = {Lab on a chip (Print)},
number = 1,
volume = 15,
place = {United States},
year = {Wed Oct 01 00:00:00 EDT 2014},
month = {Wed Oct 01 00:00:00 EDT 2014}
}
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Cited by: 34 works
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Figures / Tables:
Figure 1: Air-matrix digital microfluidics (DMF) and evaporation management solution. (a) Schematic of our air-matrix DMF device. Zoom-in windows are in side-view orientation. Highlighted features include a thermoelectric module and resistive temperature detector (RTD) mounted on the back side of the device, for temperature control; voids between the thermal zones,more »
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Figures / Tables found in this record:
Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.