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Title: MCM ring hexamerization is a prerequisite for DNA-binding

Abstract

The hexameric Minichromosome Maintenance (MCM) protein complex forms a ring that unwinds DNA at the replication fork in eukaryotes and archaea. Our recent crystal structure of an archaeal MCM N-terminal domain bound to single-stranded DNA (ssDNA) revealed ssDNA associating across tight subunit interfaces but not at the loose interfaces, indicating that DNA-binding is governed not only by the DNA-binding residues of the subunits (MCM ssDNA-binding motif, MSSB) but also by the relative orientation of the subunits. We now extend these findings to show that DNA-binding by the MCM N-terminal domain of the archaeal organism Pyrococcus furiosus occurs specifically in the hexameric oligomeric form. We show that mutants defective for hexamerization are defective in binding ssDNA despite retaining all the residues observed to interact with ssDNA in the crystal structure. One mutation that exhibits severely defective hexamerization and ssDNA-binding is at a conserved phenylalanine that aligns with the mouse Mcm4(Chaos3) mutation associated with chromosomal instability, cancer, and decreased intersubunit association.

Authors:
 [1];  [1];  [1]
+ Show Author Affiliations
  1. St. Jude Children's Research Hospital, Memphis, TN (United States)
Publication Date:
Research Org.:
Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1223715
Grant/Contract Number:  
AC02-06CH11357; W-31-109-ENG-38
Resource Type:
Accepted Manuscript
Journal Name:
Nucleic Acids Research
Additional Journal Information:
Journal Name: Nucleic Acids Research; Journal ID: ISSN 0305-1048
Publisher:
Oxford University Press
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Froelich, Clifford A., Nourse, Amanda, and Enemark, Eric J. MCM ring hexamerization is a prerequisite for DNA-binding. United States: N. p., 2015. Web. doi:10.1093/nar/gkv914.
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Froelich, Clifford A., Nourse, Amanda, & Enemark, Eric J. MCM ring hexamerization is a prerequisite for DNA-binding. United States. https://doi.org/10.1093/nar/gkv914
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Froelich, Clifford A., Nourse, Amanda, and Enemark, Eric J. Sun . "MCM ring hexamerization is a prerequisite for DNA-binding". United States. https://doi.org/10.1093/nar/gkv914. https://www.osti.gov/servlets/purl/1223715.
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@article{osti_1223715,
title = {MCM ring hexamerization is a prerequisite for DNA-binding},
author = {Froelich, Clifford A. and Nourse, Amanda and Enemark, Eric J.},
abstractNote = {The hexameric Minichromosome Maintenance (MCM) protein complex forms a ring that unwinds DNA at the replication fork in eukaryotes and archaea. Our recent crystal structure of an archaeal MCM N-terminal domain bound to single-stranded DNA (ssDNA) revealed ssDNA associating across tight subunit interfaces but not at the loose interfaces, indicating that DNA-binding is governed not only by the DNA-binding residues of the subunits (MCM ssDNA-binding motif, MSSB) but also by the relative orientation of the subunits. We now extend these findings to show that DNA-binding by the MCM N-terminal domain of the archaeal organism Pyrococcus furiosus occurs specifically in the hexameric oligomeric form. We show that mutants defective for hexamerization are defective in binding ssDNA despite retaining all the residues observed to interact with ssDNA in the crystal structure. One mutation that exhibits severely defective hexamerization and ssDNA-binding is at a conserved phenylalanine that aligns with the mouse Mcm4(Chaos3) mutation associated with chromosomal instability, cancer, and decreased intersubunit association.},
doi = {10.1093/nar/gkv914},
journal = {Nucleic Acids Research},
number = ,
volume = ,
place = {United States},
year = {Sun Sep 13 00:00:00 EDT 2015},
month = {Sun Sep 13 00:00:00 EDT 2015}
}
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https://doi.org/10.1093/nar/gkv914
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Works referenced in this record:

The Mcm2-7 Complex Has In Vitro Helicase Activity
journal, July 2008

Activation of the MCM2-7 Helicase by Association with Cdc45 and GINS Proteins
journal, January 2010

MCM proteins and DNA replication
journal, April 2006

  • Maiorano, Domenico; Lutzmann, Malik; Méchali, Marcel
  • Current Opinion in Cell Biology, Vol. 18, Issue 2
  • DOI: 10.1016/j.ceb.2006.02.006

MCM proteins: evolution, properties, and role in DNA replication
journal, June 1998

  • Kearsey, Stephen E.; Labib, Karim
  • Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, Vol. 1398, Issue 2
  • DOI: 10.1016/S0167-4781(98)00033-5

MCM Proteins in DNA Replication
journal, June 1999

A double-hexamer archaeal minichromosome maintenance protein is an ATP-dependent DNA helicase
journal, February 2000

  • Chong, J. P. J.; Hayashi, M. K.; Simon, M. N.
  • Proceedings of the National Academy of Sciences, Vol. 97, Issue 4
  • DOI: 10.1073/pnas.030539597

Organization of the archaeal MCM complex on DNA and implications for the helicase mechanism
journal, August 2005

  • McGeoch, Adam T.; Trakselis, Michael A.; Laskey, Ronald A.
  • Nature Structural & Molecular Biology, Vol. 12, Issue 9
  • DOI: 10.1038/nsmb974

Minichromosome maintenance helicase activity is controlled by N- and C-terminal motifs and requires the ATPase domain helix-2 insert
journal, May 2006

  • Jenkinson, E. R.; Chong, J. P. J.
  • Proceedings of the National Academy of Sciences, Vol. 103, Issue 20
  • DOI: 10.1073/pnas.0509297103

Hexameric ring structure of the full-length archaeal MCM protein complex
journal, October 2003

Structural basis of the Methanothermobacter thermautotrophicus MCM helicase activity
journal, October 2006

  • Costa, Alessandro; Pape, Tillmann; van Heel, Marin
  • Nucleic Acids Research, Vol. 34, Issue 20
  • DOI: 10.1093/nar/gkl708

Differences in the Single-stranded DNA Binding Activities of MCM2-7 and MCM467
journal, September 2007

  • Bochman, Matthew L.; Schwacha, Anthony
  • Journal of Biological Chemistry, Vol. 282, Issue 46
  • DOI: 10.1074/jbc.M703824200

Concerted Loading of Mcm2–7 Double Hexamers around DNA during DNA Replication Origin Licensing
journal, November 2009

A double-hexameric MCM2-7 complex is loaded onto origin DNA during licensing of eukaryotic DNA replication
journal, November 2009

  • Evrin, C.; Clarke, P.; Zech, J.
  • Proceedings of the National Academy of Sciences, Vol. 106, Issue 48
  • DOI: 10.1073/pnas.0911500106

The structural basis for MCM2–7 helicase activation by GINS and Cdc45
journal, March 2011

  • Costa, Alessandro; Ilves, Ivar; Tamberg, Nele
  • Nature Structural & Molecular Biology, Vol. 18, Issue 4
  • DOI: 10.1038/nsmb.2004

Cryo-EM structure of a helicase loading intermediate containing ORC–Cdc6–Cdt1–MCM2-7 bound to DNA
journal, July 2013

  • Sun, Jingchuan; Evrin, Cecile; Samel, Stefan A.
  • Nature Structural & Molecular Biology, Vol. 20, Issue 8
  • DOI: 10.1038/nsmb.2629

DNA binding polarity, dimerization, and ATPase ring remodeling in the CMG helicase of the eukaryotic replisome
journal, August 2014

Structural and mechanistic insights into Mcm2–7 double-hexamer assembly and function
journal, October 2014

  • Sun, Jingchuan; Fernandez-Cid, Alejandra; Riera, Alberto
  • Genes & Development, Vol. 28, Issue 20
  • DOI: 10.1101/gad.242313.114

The structure and function of MCM from archaeal M. Thermoautotrophicum
journal, January 2003

  • Fletcher, Ryan J.; Bishop, Brooke E.; Leon, Ronald P.
  • Nature Structural & Molecular Biology, Vol. 10, Issue 3
  • DOI: 10.1038/nsb893

AAA+: A Class of Chaperone-Like ATPases Associated with the Assembly, Operation, and Disassembly of Protein Complexes
journal, January 1999

  • Neuwald, Andrew F.; Aravind, L.; Spouge, John L.
  • Genome Research, Vol. 9, Issue 1
  • DOI: 10.1101/gr.9.1.27

Evolutionary Relationships and Structural Mechanisms of aaa+ Proteins
journal, June 2006

Crystal structure of a near-full-length archaeal MCM: Functional insights for an AAA+ hexameric helicase
journal, December 2008

  • Brewster, A. S.; Wang, G.; Yu, X.
  • Proceedings of the National Academy of Sciences, Vol. 105, Issue 51
  • DOI: 10.1073/pnas.0808037105

Insights into the Architecture of the Replicative Helicase from the Structure of an Archaeal MCM Homolog
journal, February 2009

DNA-binding proteins and evolution of transcription regulation in the archaea
journal, December 1999

Structure and regulatory role of the C-terminal winged helix domain of the archaeal minichromosome maintenance complex
journal, February 2015

  • Wiedemann, Christoph; Szambowska, Anna; Häfner, Sabine
  • Nucleic Acids Research, Vol. 43, Issue 5
  • DOI: 10.1093/nar/gkv120

Structural analysis of the Sulfolobus solfataricus MCM protein N-terminal domain
journal, April 2008

  • Liu, W.; Pucci, B.; Rossi, M.
  • Nucleic Acids Research, Vol. 36, Issue 10
  • DOI: 10.1093/nar/gkn183

A conserved MCM single-stranded DNA binding element is essential for replication initiation
journal, April 2014

  • Froelich, Clifford A.; Kang, Sukhyun; Epling, Leslie B.
  • eLife, Vol. 3
  • DOI: 10.7554/eLife.01993

Coupling of DNA binding and helicase activity is mediated by a conserved loop in the MCM protein
journal, January 2008

  • Sakakibara, Nozomi; Kasiviswanathan, Rajesh; Melamud, Eugene
  • Nucleic Acids Research, Vol. 36, Issue 4
  • DOI: 10.1093/nar/gkm1160

Genetic Screen for Chromosome Instability in Mice: Mcm4 and Breast Cancer
journal, May 2007

  • Shima, Naoko; Buske, Tavanna R.; Schimenti, John C.
  • Cell Cycle, Vol. 6, Issue 10
  • DOI: 10.4161/cc.6.10.4250

A viable allele of Mcm4 causes chromosome instability and mammary adenocarcinomas in mice
journal, December 2006

  • Shima, Naoko; Alcaraz, Ana; Liachko, Ivan
  • Nature Genetics, Vol. 39, Issue 1
  • DOI: 10.1038/ng1936

Post-transcriptional homeostasis and regulation of MCM2–7 in mammalian cells
journal, February 2012

  • Chuang, Chen-Hua; Yang, Dian; Bai, Gongshi
  • Nucleic Acids Research, Vol. 40, Issue 11
  • DOI: 10.1093/nar/gks176

Overview of Current Methods in Sedimentation Velocity and Sedimentation Equilibrium Analytical Ultracentrifugation
journal, February 2013

  • Zhao, Huaying; Brautigam, Chad A.; Ghirlando, Rodolfo
  • Current Protocols in Protein Science, Vol. 71, Issue 1
  • DOI: 10.1002/0471140864.ps2012s71

Recorded scan times can limit the accuracy of sedimentation coefficients in analytical ultracentrifugation
journal, June 2013

  • Zhao, Huaying; Ghirlando, Rodolfo; Piszczek, Grzegorz
  • Analytical Biochemistry, Vol. 437, Issue 1
  • DOI: 10.1016/j.ab.2013.02.011

Analytical ultracentrifugation as a tool for studying protein interactions
journal, March 2013

On the analysis of protein self-association by sedimentation velocity analytical ultracentrifugation
journal, September 2003

Measuring Protein‐Protein Interactions by Equilibrium Sedimentation
journal, November 2007

Are fluorescence-detected sedimentation velocity data reliable?
journal, June 2013

  • Lyons, Daniel F.; Lary, Jeffrey W.; Husain, Bushra
  • Analytical Biochemistry, Vol. 437, Issue 2
  • DOI: 10.1016/j.ab.2013.02.019

NMRPipe: A multidimensional spectral processing system based on UNIX pipes
journal, November 1995

  • Delaglio, Frank; Grzesiek, Stephan; Vuister, GeertenW.
  • Journal of Biomolecular NMR, Vol. 6, Issue 3
  • DOI: 10.1007/BF00197809

Phaser crystallographic software
journal, July 2007

  • McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D.
  • Journal of Applied Crystallography, Vol. 40, Issue 4
  • DOI: 10.1107/S0021889807021206

Crystallography & NMR System: A New Software Suite for Macromolecular Structure Determination
journal, September 1998

  • Brünger, A. T.; Adams, P. D.; Clore, G. M.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 54, Issue 5
  • DOI: 10.1107/S0907444998003254

Version 1.2 of the Crystallography and NMR system
journal, October 2007

REFMAC 5 dictionary: organization of prior chemical knowledge and guidelines for its use
journal, November 2004

  • Vagin, Alexei A.; Steiner, Roberto A.; Lebedev, Andrey A.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12
  • DOI: 10.1107/S0907444904023510

PROCHECK: a program to check the stereochemical quality of protein structures
journal, April 1993

  • Laskowski, R. A.; MacArthur, M. W.; Moss, D. S.
  • Journal of Applied Crystallography, Vol. 26, Issue 2
  • DOI: 10.1107/S0021889892009944

Intersubunit allosteric communication mediated by a conserved loop in the MCM helicase
journal, January 2009

  • Barry, E. R.; Lovett, J. E.; Costa, A.
  • Proceedings of the National Academy of Sciences, Vol. 106, Issue 4
  • DOI: 10.1073/pnas.0809192106

Oligomeric States of Bacteriophage T7 Gene 4 Primase/Helicase
journal, July 2006

  • Crampton, Donald J.; Ohi, Melanie; Qimron, Udi
  • Journal of Molecular Biology, Vol. 360, Issue 3
  • DOI: 10.1016/j.jmb.2006.05.037

Structure and Function of Hexameric Helicases
journal, June 2000

Structure of the Tertiary Complex of the RepA Hexameric Helicase of Plasmid RSF1010 with the ssDNA and Nucleotide Cofactors in Solution †
journal, November 2007

  • Marcinowicz, Agnieszka; Jezewska, Maria J.; Bujalowski, Paul J.
  • Biochemistry, Vol. 46, Issue 46
  • DOI: 10.1021/bi700729k

DNA is bound within the central hole to one or two of the six subunits of the T7 DNA helicase
journal, September 1996

  • Yu, Xiong; Hingorani, Manju M.; Patel, Smita S.
  • Nature Structural & Molecular Biology, Vol. 3, Issue 9
  • DOI: 10.1038/nsb0996-740

The Phage T4-coded DNA Replication Helicase (gp41) Forms a Hexamer upon Activation by Nucleoside Triphosphate
journal, March 1995

  • Dong, Feng; Gogol, Edward P.; von Hippel, Peter H.
  • Journal of Biological Chemistry, Vol. 270, Issue 13
  • DOI: 10.1074/jbc.270.13.7462

Structure and assembly of the Escherichia coli transcription termination factor rho and its interactions with RNA I. Cryoelectron microscopic studies
journal, October 1991

  • Gogol, Edward P.; Seifried, Steven E.; von Hippel, Peter H.
  • Journal of Molecular Biology, Vol. 221, Issue 4
  • DOI: 10.1016/0022-2836(91)90923-T

The Crystal Structure of the Bifunctional Primase-Helicase of Bacteriophage T7
journal, November 2003

The Eukaryotic Mcm2-7 Replicative Helicase
book, January 2012

Structure of the eukaryotic MCM complex at 3.8 Å
journal, July 2015

  • Li, Ningning; Zhai, Yuanliang; Zhang, Yixiao
  • Nature, Vol. 524, Issue 7564
  • DOI: 10.1038/nature14685

Are fluorescence-detected sedimentation velocity data reliable?
journal, June 2013

  • Lyons, Daniel F.; Lary, Jeffrey W.; Husain, Bushra
  • Analytical Biochemistry, Vol. 437, Issue 2
  • DOI: 10.1016/j.ab.2013.02.019

Putting Two Heads Together to Unwind DNA
journal, November 2009

Oligomeric States of Bacteriophage T7 Gene 4 Primase/Helicase
journal, July 2006

  • Crampton, Donald J.; Ohi, Melanie; Qimron, Udi
  • Journal of Molecular Biology, Vol. 360, Issue 3
  • DOI: 10.1016/j.jmb.2006.05.037

Activation of the MCM2-7 Helicase by Association with Cdc45 and GINS Proteins
journal, January 2010

Insights into the Architecture of the Replicative Helicase from the Structure of an Archaeal MCM Homolog
journal, February 2009

Crystal Structure of T7 Gene 4 Ring Helicase Indicates a Mechanism for Sequential Hydrolysis of Nucleotides
journal, June 2000

Structure of the Tertiary Complex of the RepA Hexameric Helicase of Plasmid RSF1010 with the ssDNA and Nucleotide Cofactors in Solution †
journal, November 2007

  • Marcinowicz, Agnieszka; Jezewska, Maria J.; Bujalowski, Paul J.
  • Biochemistry, Vol. 46, Issue 46
  • DOI: 10.1021/bi700729k

DNA is bound within the central hole to one or two of the six subunits of the T7 DNA helicase
journal, September 1996

  • Yu, Xiong; Hingorani, Manju M.; Patel, Smita S.
  • Nature Structural & Molecular Biology, Vol. 3, Issue 9
  • DOI: 10.1038/nsb0996-740

The structural basis for MCM2–7 helicase activation by GINS and Cdc45
journal, March 2011

  • Costa, Alessandro; Ilves, Ivar; Tamberg, Nele
  • Nature Structural & Molecular Biology, Vol. 18, Issue 4
  • DOI: 10.1038/nsmb.2004

Cryo-EM structure of a helicase loading intermediate containing ORC–Cdc6–Cdt1–MCM2-7 bound to DNA
journal, July 2013

  • Sun, Jingchuan; Evrin, Cecile; Samel, Stefan A.
  • Nature Structural & Molecular Biology, Vol. 20, Issue 8
  • DOI: 10.1038/nsmb.2629

Organization of the archaeal MCM complex on DNA and implications for the helicase mechanism
journal, August 2005

  • McGeoch, Adam T.; Trakselis, Michael A.; Laskey, Ronald A.
  • Nature Structural & Molecular Biology, Vol. 12, Issue 9
  • DOI: 10.1038/nsmb974

A double-hexamer archaeal minichromosome maintenance protein is an ATP-dependent DNA helicase
journal, February 2000

  • Chong, J. P. J.; Hayashi, M. K.; Simon, M. N.
  • Proceedings of the National Academy of Sciences, Vol. 97, Issue 4
  • DOI: 10.1073/pnas.030539597

Minichromosome maintenance helicase activity is controlled by N- and C-terminal motifs and requires the ATPase domain helix-2 insert
journal, May 2006

  • Jenkinson, E. R.; Chong, J. P. J.
  • Proceedings of the National Academy of Sciences, Vol. 103, Issue 20
  • DOI: 10.1073/pnas.0509297103

Crystal structure of a near-full-length archaeal MCM: Functional insights for an AAA+ hexameric helicase
journal, December 2008

  • Brewster, A. S.; Wang, G.; Yu, X.
  • Proceedings of the National Academy of Sciences, Vol. 105, Issue 51
  • DOI: 10.1073/pnas.0808037105

A double-hexameric MCM2-7 complex is loaded onto origin DNA during licensing of eukaryotic DNA replication
journal, November 2009

  • Evrin, C.; Clarke, P.; Zech, J.
  • Proceedings of the National Academy of Sciences, Vol. 106, Issue 48
  • DOI: 10.1073/pnas.0911500106

The Phage T4-coded DNA Replication Helicase (gp41) Forms a Hexamer upon Activation by Nucleoside Triphosphate
journal, March 1995

  • Dong, Feng; Gogol, Edward P.; von Hippel, Peter H.
  • Journal of Biological Chemistry, Vol. 270, Issue 13
  • DOI: 10.1074/jbc.270.13.7462

Characterization and Structure Determination of the Cdt1 Binding Domain of Human Minichromosome Maintenance (Mcm) 6
journal, March 2010

  • Wei, Zhun; Liu, Changdong; Wu, Xing
  • Journal of Biological Chemistry, Vol. 285, Issue 17
  • DOI: 10.1074/jbc.c109.094599

Physical and Functional Interaction between the Mini-chromosome Maintenance-like DNA Helicase and the Single-stranded DNA Binding Protein from the Crenarchaeon Sulfolobus solfataricus
journal, January 2002

  • Carpentieri, Floriana; De Felice, Mariarita; De Falco, Mariarosaria
  • Journal of Biological Chemistry, Vol. 277, Issue 14
  • DOI: 10.1074/jbc.m200091200

Biochemical Characterization of the Methanothermobacter thermautotrophicus Minichromosome Maintenance (MCM) Helicase N-terminal Domains
journal, April 2004

  • Kasiviswanathan, Rajesh; Shin, Jae-Ho; Melamud, Eugene
  • Journal of Biological Chemistry, Vol. 279, Issue 27
  • DOI: 10.1074/jbc.m403202200

Amino Acids of the Sulfolobus solfataricus Mini-chromosome Maintenance-like DNA Helicase Involved in DNA Binding/Remodeling
journal, September 2004

  • Pucci, Biagio; De Felice, Mariarita; Rossi, Mosè
  • Journal of Biological Chemistry, Vol. 279, Issue 47
  • DOI: 10.1074/jbc.m408967200

Polymorphism and Double Hexamer Structure in the Archaeal Minichromosome Maintenance (MCM) Helicase from Methanobacterium thermoautotrophicum
journal, October 2005

  • Gómez-Llorente, Yacob; Fletcher, Ryan J.; Chen, Xiaojiang S.
  • Journal of Biological Chemistry, Vol. 280, Issue 49
  • DOI: 10.1074/jbc.m509760200

Modular Organization of the Sulfolobus solfataricus Mini-chromosome Maintenance Protein
journal, March 2007

  • Pucci, Biagio; De Felice, Mariarita; Rocco, Monia
  • Journal of Biological Chemistry, Vol. 282, Issue 17
  • DOI: 10.1074/jbc.m610953200

DNA-binding proteins and evolution of transcription regulation in the archaea
journal, December 1999

Structural basis of the Methanothermobacter thermautotrophicus MCM helicase activity
journal, October 2006

  • Costa, Alessandro; Pape, Tillmann; van Heel, Marin
  • Nucleic Acids Research, Vol. 34, Issue 20
  • DOI: 10.1093/nar/gkl708

Coupling of DNA binding and helicase activity is mediated by a conserved loop in the MCM protein
journal, January 2008

  • Sakakibara, Nozomi; Kasiviswanathan, Rajesh; Melamud, Eugene
  • Nucleic Acids Research, Vol. 36, Issue 4
  • DOI: 10.1093/nar/gkm1160

Structural analysis of the Sulfolobus solfataricus MCM protein N-terminal domain
journal, April 2008

  • Liu, W.; Pucci, B.; Rossi, M.
  • Nucleic Acids Research, Vol. 36, Issue 10
  • DOI: 10.1093/nar/gkn183

Post-transcriptional homeostasis and regulation of MCM2–7 in mammalian cells
journal, February 2012

  • Chuang, Chen-Hua; Yang, Dian; Bai, Gongshi
  • Nucleic Acids Research, Vol. 40, Issue 11
  • DOI: 10.1093/nar/gks176

Structure and regulatory role of the C-terminal winged helix domain of the archaeal minichromosome maintenance complex
journal, February 2015

  • Wiedemann, Christoph; Szambowska, Anna; Häfner, Sabine
  • Nucleic Acids Research, Vol. 43, Issue 5
  • DOI: 10.1093/nar/gkv120

Subunit Organization of Mcm2-7 and the Unequal Role of Active Sites in ATP Hydrolysis and Viability
journal, July 2008

  • Bochman, Matthew L.; Bell, Stephen P.; Schwacha, Anthony
  • Molecular and Cellular Biology, Vol. 28, Issue 19
  • DOI: 10.1128/mcb.00161-08

Structure and Function of Hexameric Helicases
journal, June 2000

Different Residues on the Surface of theMethanothermobacter thermautotrophicusMCM Helicase Interact with Single- and Double-Stranded DNA
journal, January 2010

  • Sakakibara, Nozomi; Kasiviswanathan, Rajesh; Kelman, Zvi
  • Archaea, Vol. 2010
  • DOI: 10.1155/2010/505693

Genetic Screen for Chromosome Instability in Mice: Mcm4 and Breast Cancer
journal, May 2007

  • Shima, Naoko; Buske, Tavanna R.; Schimenti, John C.
  • Cell Cycle, Vol. 6, Issue 10
  • DOI: 10.4161/cc.6.10.4250

Preparing to unwind
journal, April 2014

Analysis of the crystal structure of an active MCM hexamer
journal, September 2014

  • Miller, Justin M.; Arachea, Buenafe T.; Epling, Leslie B.
  • eLife, Vol. 3
  • DOI: 10.7554/elife.03433
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