Bacillus anthracis spores germinate extracellularly at air-liquid interface in an in vitro lung model under serum-free conditions
Abstract
Aims: To better understand the parameters that govern spore dissemination after lung exposure using in vitro cell systems. Methods and Results: We evaluated the kinetics of uptake, germination and proliferation of B. anthracis Sterne spores in association with human primary lung epithelial cells, Calu-3, and A549 cell lines. We also analyzed the influence of various cell culture media formulations related to spore germination. Conclusions: We found negligible spore uptake by epithelial cells, but germination and proliferation of spores in the extracellular environment was evident, and was appreciably higher in A549 and Calu-3 cultures than in primary epithelial cells. Additionally, our results revealed spores in association with primary cells submerged in cell culture media germinated 1 h
- Authors:
-
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Chemical and Biological Signature Sciences Group
- Publication Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1222891
- Report Number(s):
- PNNL-SA-109060
400904120
- Grant/Contract Number:
- AC05-76RL01830
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Applied Microbiology, 119(3):711-723
- Additional Journal Information:
- Journal Name: Journal of Applied Microbiology, 119(3):711-723
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Powell, Joshua D., Hutchison, Janine R., Hess, Becky M., and Straub, Tim M. Bacillus anthracis spores germinate extracellularly at air-liquid interface in an in vitro lung model under serum-free conditions. United States: N. p., 2015.
Web. doi:10.1111/jam.12872.
Powell, Joshua D., Hutchison, Janine R., Hess, Becky M., & Straub, Tim M. Bacillus anthracis spores germinate extracellularly at air-liquid interface in an in vitro lung model under serum-free conditions. United States. https://doi.org/10.1111/jam.12872
Powell, Joshua D., Hutchison, Janine R., Hess, Becky M., and Straub, Tim M. Thu .
"Bacillus anthracis spores germinate extracellularly at air-liquid interface in an in vitro lung model under serum-free conditions". United States. https://doi.org/10.1111/jam.12872. https://www.osti.gov/servlets/purl/1222891.
@article{osti_1222891,
title = {Bacillus anthracis spores germinate extracellularly at air-liquid interface in an in vitro lung model under serum-free conditions},
author = {Powell, Joshua D. and Hutchison, Janine R. and Hess, Becky M. and Straub, Tim M.},
abstractNote = {Aims: To better understand the parameters that govern spore dissemination after lung exposure using in vitro cell systems. Methods and Results: We evaluated the kinetics of uptake, germination and proliferation of B. anthracis Sterne spores in association with human primary lung epithelial cells, Calu-3, and A549 cell lines. We also analyzed the influence of various cell culture media formulations related to spore germination. Conclusions: We found negligible spore uptake by epithelial cells, but germination and proliferation of spores in the extracellular environment was evident, and was appreciably higher in A549 and Calu-3 cultures than in primary epithelial cells. Additionally, our results revealed spores in association with primary cells submerged in cell culture media germinated 1 h},
doi = {10.1111/jam.12872},
journal = {Journal of Applied Microbiology, 119(3):711-723},
number = ,
volume = ,
place = {United States},
year = {Thu Jul 30 00:00:00 EDT 2015},
month = {Thu Jul 30 00:00:00 EDT 2015}
}
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