Engineering photosynthetic organisms for the production of biohydrogen
Abstract
Oxygenic photosynthetic organisms such as green algae are capable of absorbing sunlight and converting the chemical energy into hydrogen gas. This process takes advantage of the photosynthetic apparatus of these organisms which links water oxidation to H2 production. Biological H2 has therefore the potential to be an alternative fuel of the future and shows great promise for generating large scale sustainable energy. Microalgae are able to produce H2 under light anoxic or dark anoxic condition by activating 3 different pathways that utilize the hydrogenases as catalysts. In this review, we highlight the principal barriers that prevent hydrogen production in green algae and how those limitations are being addressed, through metabolic and genetic engineering. We also discuss the major challenges and bottlenecks facing the development of future commercial algal photobiological systems for H2 production. Lastly we provide suggestions for future strategies and potential new techniques to be developed towards an integrated system with optimized hydrogen production.
- Authors:
-
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Publication Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1220584
- Report Number(s):
- NREL/JA-2700-60901
Journal ID: ISSN 0166-8595
- Grant/Contract Number:
- AC36-08GO28308
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Photosynthesis Research
- Additional Journal Information:
- Journal Volume: 123; Journal Issue: 3; Related Information: Photosynthesis Research; Journal ID: ISSN 0166-8595
- Publisher:
- Springer
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; green algae; H2 metabolism; hydrogenases; electron transfer; genetic engineering
Citation Formats
Dubini, Alexandra, and Ghirardi, Maria L. Engineering photosynthetic organisms for the production of biohydrogen. United States: N. p., 2014.
Web. doi:10.1007/s11120-014-9991-x.
Dubini, Alexandra, & Ghirardi, Maria L. Engineering photosynthetic organisms for the production of biohydrogen. United States. https://doi.org/10.1007/s11120-014-9991-x
Dubini, Alexandra, and Ghirardi, Maria L. Thu .
"Engineering photosynthetic organisms for the production of biohydrogen". United States. https://doi.org/10.1007/s11120-014-9991-x. https://www.osti.gov/servlets/purl/1220584.
@article{osti_1220584,
title = {Engineering photosynthetic organisms for the production of biohydrogen},
author = {Dubini, Alexandra and Ghirardi, Maria L.},
abstractNote = {Oxygenic photosynthetic organisms such as green algae are capable of absorbing sunlight and converting the chemical energy into hydrogen gas. This process takes advantage of the photosynthetic apparatus of these organisms which links water oxidation to H2 production. Biological H2 has therefore the potential to be an alternative fuel of the future and shows great promise for generating large scale sustainable energy. Microalgae are able to produce H2 under light anoxic or dark anoxic condition by activating 3 different pathways that utilize the hydrogenases as catalysts. In this review, we highlight the principal barriers that prevent hydrogen production in green algae and how those limitations are being addressed, through metabolic and genetic engineering. We also discuss the major challenges and bottlenecks facing the development of future commercial algal photobiological systems for H2 production. Lastly we provide suggestions for future strategies and potential new techniques to be developed towards an integrated system with optimized hydrogen production.},
doi = {10.1007/s11120-014-9991-x},
journal = {Photosynthesis Research},
number = 3,
volume = 123,
place = {United States},
year = {Thu Mar 27 00:00:00 EDT 2014},
month = {Thu Mar 27 00:00:00 EDT 2014}
}
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Improved biohydrogen production with an expression of codon-optimized hemH and lba genes in the chloroplast of Chlamydomonas reinhardtii
journal, February 2011
- Wu, Shuangxiu; Xu, Lili; Huang, Rui
- Bioresource Technology, Vol. 102, Issue 3
Knockdown of PsbO leads to induction of HydA and production of photobiological H2 in the green alga Chlorella sp. DT
journal, September 2013
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Increased hydrogen photoproduction by means of a sulfur-deprived Chlamydomonas reinhardtii D1 protein mutant
journal, May 2009
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Relationships between PSII-independent hydrogen bioproduction and starch metabolism as evidenced from isolation of starch catabolism mutants in the green alga Chlamydomonas reinhardtii
journal, October 2010
- Chochois, Vincent; Constans, Laure; Dauvillée, David
- International Journal of Hydrogen Energy, Vol. 35, Issue 19
A truncated antenna mutant of Chlamydomonas reinhardtii can produce more hydrogen than the parental strain
journal, February 2011
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- International Journal of Hydrogen Energy, Vol. 36, Issue 3
Design of a new biosensor for algal H2 production based on the H2-sensing system of Rhodobacter capsulatus
journal, August 2011
- Wecker, Matt S. A.; Meuser, Jonathan E.; Posewitz, Matthew C.
- International Journal of Hydrogen Energy, Vol. 36, Issue 17
Introducing pyruvate oxidase into the chloroplast of Chlamydomonas reinhardtii increases oxygen consumption and promotes hydrogen production
journal, August 2011
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- International Journal of Hydrogen Energy, Vol. 36, Issue 17
Solar-to-bioH2 production enhanced by homologous overexpression of hydrogenase in green alga Chlorella sp. DT
journal, December 2012
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- International Journal of Hydrogen Energy, Vol. 37, Issue 23
Functional integration of the HUP1 hexose symporter gene into the genome of C. reinhardtii: Impacts on biological H2 production
journal, August 2007
- Doebbe, Anja; Rupprecht, Jens; Beckmann, Julia
- Journal of Biotechnology, Vol. 131, Issue 1
Improved hydrogen production with expression of hemH and lba genes in chloroplast of Chlamydomonas reinhardtii
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- Journal of Biotechnology, Vol. 146, Issue 3
Sustained H2 production in a Chlamydomonas reinhardtii D1 protein mutant
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Aspartate 170 of the photosystem II reaction center polypeptide D1 is involved in the assembly of the oxygen-evolving manganese cluster
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- Biochemistry, Vol. 31, Issue 3
Hydrogenase/Ferredoxin Charge-Transfer Complexes: Effect of Hydrogenase Mutations on the Complex Association †
journal, April 2009
- Long, Hai; King, Paul W.; Ghirardi, Maria L.
- The Journal of Physical Chemistry A, Vol. 113, Issue 16
Relating diffusion along the substrate tunnel and oxygen sensitivity in hydrogenase
journal, December 2009
- Liebgott, Pierre-Pol; Leroux, Fanny; Burlat, Bénédicte
- Nature Chemical Biology, Vol. 6, Issue 1
Cyclic electron flow is redox-controlled but independent of state transition
journal, June 2013
- Takahashi, Hiroko; Clowez, Sophie; Wollman, Francis-André
- Nature Communications, Vol. 4, Issue 1
The synthesis of Rhodobacter capsulatus HupSL hydrogenase is regulated by the two-component HupT/HupR system
journal, December 1999
- Dischert, Wanda; Vignais, Paulette M.; Colbeau, Annette
- Molecular Microbiology, Vol. 34, Issue 5
Potential for hydrogen production with inducible chloroplast gene expression in Chlamydomonas
journal, October 2007
- Surzycki, R.; Cournac, L.; Peltier, G.
- Proceedings of the National Academy of Sciences, Vol. 104, Issue 44
Photosynthetic electron partitioning between [FeFe]-hydrogenase and ferredoxin:NADP+-oxidoreductase (FNR) enzymes in vitro
journal, May 2011
- Yacoby, I.; Pochekailov, S.; Toporik, H.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 23
Increased photosystem II stability promotes H2 production in sulfur-deprived Chlamydomonas reinhardtii
journal, April 2013
- Volgusheva, A.; Styring, S.; Mamedov, F.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 18
Biophysical, Biochemical, and Physiological Characterization of Chlamydomonas reinhardtii Mutants with Amino Acid Substitutions at the Ala 251 Residue in the D1 Protein That Result in Varying Levels of Photosynthetic Competence
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