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Title: Structural prerequisites for G-protein activation by the neurotensin receptor

Abstract

We previously determined the structure of neurotensin receptor NTSR1 in an active-like conformation with six thermostabilizing mutations bound to the peptide agonist neurotensin. This receptor was unable to activate G proteins, indicating that the mutations restricted NTSR1 to relate agonist binding to G-protein activation. Here we analyse the effect of three of those mutations (E166A3.49, L310A6.37, F358A7.42) and present two structures of NTSR1 able to catalyse nucleotide exchange at Gα. The presence of F3587.42 causes the conserved W3216.48 to adopt a side chain orientation parallel to the lipid bilayer sealing the collapsed Na+ ion pocket and linking the agonist with residues in the lower receptor part implicated in GPCR activation. In the intracellular receptor half, the bulkier L3106.37 side chain dictates the position of R1673.50 of the highly conserved D/ERY motif. These residues, together with the presence of E1663.49 provide determinants for G-protein activation by NTSR1.

Authors:
 [1];  [1];  [1];  [1]
+ Show Author Affiliations
  1. National Inst. of Health (NIH), Rockville, MD (United States). Dept. of Health and Human Services
Publication Date:
Research Org.:
Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS); SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)
Sponsoring Org.:
National Institutes of Health (NIH); USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1203746
Grant/Contract Number:  
AC02-06CH11357; AC02-76SF00515
Resource Type:
Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 6; Journal Issue: 07, 2015; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Krumm, Brian E., White, Jim F., Shah, Priyanka, and Grisshammer, Reinhard. Structural prerequisites for G-protein activation by the neurotensin receptor. United States: N. p., 2015. Web. doi:10.1038/ncomms8895.
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Krumm, Brian E., White, Jim F., Shah, Priyanka, & Grisshammer, Reinhard. Structural prerequisites for G-protein activation by the neurotensin receptor. United States. https://doi.org/10.1038/ncomms8895
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Krumm, Brian E., White, Jim F., Shah, Priyanka, and Grisshammer, Reinhard. Fri . "Structural prerequisites for G-protein activation by the neurotensin receptor". United States. https://doi.org/10.1038/ncomms8895. https://www.osti.gov/servlets/purl/1203746.
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@article{osti_1203746,
title = {Structural prerequisites for G-protein activation by the neurotensin receptor},
author = {Krumm, Brian E. and White, Jim F. and Shah, Priyanka and Grisshammer, Reinhard},
abstractNote = {We previously determined the structure of neurotensin receptor NTSR1 in an active-like conformation with six thermostabilizing mutations bound to the peptide agonist neurotensin. This receptor was unable to activate G proteins, indicating that the mutations restricted NTSR1 to relate agonist binding to G-protein activation. Here we analyse the effect of three of those mutations (E166A3.49, L310A6.37, F358A7.42) and present two structures of NTSR1 able to catalyse nucleotide exchange at Gα. The presence of F3587.42 causes the conserved W3216.48 to adopt a side chain orientation parallel to the lipid bilayer sealing the collapsed Na+ ion pocket and linking the agonist with residues in the lower receptor part implicated in GPCR activation. In the intracellular receptor half, the bulkier L3106.37 side chain dictates the position of R1673.50 of the highly conserved D/ERY motif. These residues, together with the presence of E1663.49 provide determinants for G-protein activation by NTSR1.},
doi = {10.1038/ncomms8895},
journal = {Nature Communications},
number = 07, 2015,
volume = 6,
place = {United States},
year = {Fri Jul 24 00:00:00 EDT 2015},
month = {Fri Jul 24 00:00:00 EDT 2015}
}
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Works referenced in this record:

Specific Tryptophan UV-Absorbance Changes Are Probes of the Transition of Rhodopsin to Its Active State †
journal, January 1996

  • Lin, Steven W.; Sakmar, Thomas P.
  • Biochemistry, Vol. 35, Issue 34
  • DOI: 10.1021/bi960858u

High-resolution crystal structure of human protease-activated receptor 1
journal, December 2012

  • Zhang, Cheng; Srinivasan, Yoga; Arlow, Daniel H.
  • Nature, Vol. 492, Issue 7429
  • DOI: 10.1038/nature11701

Thermostabilization of the Neurotensin Receptor NTS1
journal, July 2009

  • Shibata, Yoko; White, Jim F.; Serrano-Vega, Maria J.
  • Journal of Molecular Biology, Vol. 390, Issue 2
  • DOI: 10.1016/j.jmb.2009.04.068

Overview of the CCP 4 suite and current developments
journal, March 2011

  • Winn, Martyn D.; Ballard, Charles C.; Cowtan, Kevin D.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4
  • DOI: 10.1107/S0907444910045749

Critical features for biosynthesis, stability, and functionality of a G protein-coupled receptor uncovered by all-versus-all mutations
journal, June 2012

  • Schlinkmann, K. M.; Honegger, A.; Tureci, E.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 25
  • DOI: 10.1073/pnas.1202107109

Amino acid conservation and interactions in rhodopsin: Probing receptor activation by NMR spectroscopy
journal, May 2014

  • Pope, Andreyah; Eilers, Markus; Reeves, Philip J.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1837, Issue 5
  • DOI: 10.1016/j.bbabio.2013.10.007

Structure and functional expression of the cloned rat neurotensin receptor
journal, June 1990

Lipidic cubic phases: A novel concept for the crystallization of membrane proteins
journal, December 1996

  • Landau, E. M.; Rosenbusch, J. P.
  • Proceedings of the National Academy of Sciences, Vol. 93, Issue 25
  • DOI: 10.1073/pnas.93.25.14532

Rastering strategy for screening and centring of microcrystal samples of human membrane proteins with a sub-10 µm size X-ray synchrotron beam
journal, June 2009

  • Cherezov, Vadim; Hanson, Michael A.; Griffith, Mark T.
  • Journal of The Royal Society Interface, Vol. 6, Issue suppl_5
  • DOI: 10.1098/rsif.2009.0142.focus

Crystal structure of opsin in its G-protein-interacting conformation
journal, September 2008

  • Scheerer, Patrick; Park, Jung Hee; Hildebrand, Peter W.
  • Nature, Vol. 455, Issue 7212
  • DOI: 10.1038/nature07330

Neuropeptide receptor ligands as drugs for psychiatric diseases: the end of the beginning?
journal, May 2012

  • Griebel, Guy; Holsboer, Florian
  • Nature Reviews Drug Discovery, Vol. 11, Issue 6
  • DOI: 10.1038/nrd3702

Maximizing Detergent Stability and Functional Expression of a GPCR by Exhaustive Recombination and Evolution
journal, September 2012

  • Schlinkmann, Karola M.; Hillenbrand, Matthias; Rittner, Alexander
  • Journal of Molecular Biology, Vol. 422, Issue 3
  • DOI: 10.1016/j.jmb.2012.05.039

The Bombesin Receptor Subtypes Have Distinct G Protein Specificities
journal, April 1999

  • Jian, Xiaoying; Sainz, Eduardo; Clark, William A.
  • Journal of Biological Chemistry, Vol. 274, Issue 17
  • DOI: 10.1074/jbc.274.17.11573

Biochemical and pharmacological profile of a potent and selective nonpeptide antagonist of the neurotensin receptor.
journal, January 1993

  • Gully, D.; Canton, M.; Boigegrain, R.
  • Proceedings of the National Academy of Sciences, Vol. 90, Issue 1
  • DOI: 10.1073/pnas.90.1.65

Optimising the combination of thermostabilising mutations in the neurotensin receptor for structure determination
journal, April 2013

  • Shibata, Yoko; Gvozdenovic-Jeremic, Jelena; Love, James
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1828, Issue 4
  • DOI: 10.1016/j.bbamem.2013.01.008

Structure of the agonist-bound neurotensin receptor
journal, October 2012

  • White, Jim F.; Noinaj, Nicholas; Shibata, Yoko
  • Nature, Vol. 490, Issue 7421
  • DOI: 10.1038/nature11558

Crystallizing membrane proteins using lipidic mesophases
journal, April 2009

XDS
journal, January 2010

  • Kabsch, Wolfgang
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2
  • DOI: 10.1107/S0907444909047337

Structure of a β1-adrenergic G-protein-coupled receptor
journal, June 2008

  • Warne, Tony; Serrano-Vega, Maria J.; Baker, Jillian G.
  • Nature, Vol. 454, Issue 7203
  • DOI: 10.1038/nature07101

The structural basis of agonist-induced activation in constitutively active rhodopsin
journal, March 2011

  • Standfuss, Jörg; Edwards, Patricia C.; D’Antona, Aaron
  • Nature, Vol. 471, Issue 7340
  • DOI: 10.1038/nature09795

Pivotal Role of an Aspartate Residue in Sodium Sensitivity and Coupling to G Proteins of Neurotensin Receptors
journal, February 1999

  • Martin, Stéphane; Botto, Jean-Marie; Vincent, Jean-Pierre
  • Molecular Pharmacology, Vol. 55, Issue 2
  • DOI: 10.1124/mol.55.2.210

Structure of a nanobody-stabilized active state of the β2 adrenoceptor
journal, January 2011

  • Rasmussen, Søren G. F.; Choi, Hee-Jung; Fung, Juan Jose
  • Nature, Vol. 469, Issue 7329
  • DOI: 10.1038/nature09648

Crystal structure of the µ-opioid receptor bound to a morphinan antagonist
journal, March 2012

  • Manglik, Aashish; Kruse, Andrew C.; Kobilka, Tong Sun
  • Nature, Vol. 485, Issue 7398
  • DOI: 10.1038/nature10954

Activation and allosteric modulation of a muscarinic acetylcholine receptor
journal, November 2013

  • Kruse, Andrew C.; Ring, Aaron M.; Manglik, Aashish
  • Nature, Vol. 504, Issue 7478
  • DOI: 10.1038/nature12735

MolProbity : all-atom structure validation for macromolecular crystallography
journal, December 2009

  • Chen, Vincent B.; Arendall, W. Bryan; Headd, Jeffrey J.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 1
  • DOI: 10.1107/S0907444909042073

Crystal structure of the β2 adrenergic receptor–Gs protein complex
journal, July 2011

  • Rasmussen, Søren G. F.; DeVree, Brian T.; Zou, Yaozhong
  • Nature, Vol. 477, Issue 7366
  • DOI: 10.1038/nature10361

Structure of a β1-adrenergic G-protein-coupled receptor
journal, June 2008

  • Warne, Tony; Serrano-Vega, Maria J.; Baker, Jillian G.
  • Nature, Vol. 454, Issue 7203
  • DOI: 10.1038/nature07101

PHENIX: a comprehensive Python-based system for macromolecular structure solution
journal, January 2010

  • Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221
  • DOI: 10.1107/S0907444909052925

Molecular control of δ-opioid receptor signalling
journal, January 2014

  • Fenalti, Gustavo; Giguere, Patrick M.; Katritch, Vsevolod
  • Nature, Vol. 506, Issue 7487
  • DOI: 10.1038/nature12944

Automated sample-scanning methods for radiation damage mitigation and diffraction-based centering of macromolecular crystals
journal, July 2011

  • Hilgart, Mark C.; Sanishvili, Ruslan; Ogata, Craig M.
  • Journal of Synchrotron Radiation, Vol. 18, Issue 5
  • DOI: 10.1107/S0909049511029918

Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity
journal, September 2008

  • Sarkar, C. A.; Dodevski, I.; Kenig, M.
  • Proceedings of the National Academy of Sciences, Vol. 105, Issue 39
  • DOI: 10.1073/pnas.0803103105

Functional Reconstitution in Situ of 5-Hydroxytryptamine 2c (5HT 2c ) Receptors with α q and Inverse Agonism of 5HT 2c Receptor Antagonists
journal, September 1996

A crystal clear solution for determining G-protein-coupled receptor structures
journal, September 2012

Optimal description of a protein structure in terms of multiple groups undergoing TLS motion
journal, March 2006

  • Painter, Jay; Merritt, Ethan A.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 62, Issue 4
  • DOI: 10.1107/S0907444906005270

Unifying Family A GPCR Theories of Activation
journal, July 2014

High-Resolution Crystal Structure of an Engineered Human  2-Adrenergic G Protein-Coupled Receptor
journal, October 2007

Optimising the combination of thermostabilising mutations in the neurotensin receptor for structure determination
journal, April 2013

  • Shibata, Yoko; Gvozdenovic-Jeremic, Jelena; Love, James
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1828, Issue 4
  • DOI: 10.1016/j.bbamem.2013.01.008

Specific Tryptophan UV-Absorbance Changes Are Probes of the Transition of Rhodopsin to Its Active State †
journal, January 1996

  • Lin, Steven W.; Sakmar, Thomas P.
  • Biochemistry, Vol. 35, Issue 34
  • DOI: 10.1021/bi960858u

Amino acid conservation and interactions in rhodopsin: Probing receptor activation by NMR spectroscopy
journal, May 2014

  • Pope, Andreyah; Eilers, Markus; Reeves, Philip J.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1837, Issue 5
  • DOI: 10.1016/j.bbabio.2013.10.007

PHENIX: a comprehensive Python-based system for macromolecular structure solution.
text, January 2010

  • Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
  • Apollo - University of Cambridge Repository
  • DOI: 10.17863/cam.45787

Selective reconstitution of gastrin-releasing peptide receptor with G q
journal, January 1997

  • Hellmich, M. R.; Battey, J. F.; Northup, J. K.
  • Proceedings of the National Academy of Sciences, Vol. 94, Issue 2
  • DOI: 10.1073/pnas.94.2.751

Structural Dynamics and Thermostabilization of Neurotensin Receptor 1
journal, April 2015

  • Lee, Sangbae; Bhattacharya, Supriyo; Tate, Christopher G.
  • The Journal of Physical Chemistry B, Vol. 119, Issue 15
  • DOI: 10.1021/jp510735f

Thermostabilization of the Neurotensin Receptor NTS1
journal, July 2009

  • Shibata, Yoko; White, Jim F.; Serrano-Vega, Maria J.
  • Journal of Molecular Biology, Vol. 390, Issue 2
  • DOI: 10.1016/j.jmb.2009.04.068

Unifying Family A GPCR Theories of Activation
journal, July 2014

Dependence of critical micelle concentration of a zwitterionic detergent on ionic strength: implications in receptor solubilization
journal, August 1996

Critical features for biosynthesis, stability, and functionality of a G protein-coupled receptor uncovered by all-versus-all mutations
journal, June 2012

  • Schlinkmann, K. M.; Honegger, A.; Tureci, E.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 25
  • DOI: 10.1073/pnas.1202107109

The structural basis of agonist-induced activation in constitutively active rhodopsin
journal, March 2011

  • Standfuss, Jörg; Edwards, Patricia C.; D’Antona, Aaron
  • Nature, Vol. 471, Issue 7340
  • DOI: 10.1038/nature09795

Structure of the agonist-bound neurotensin receptor
journal, October 2012

  • White, Jim F.; Noinaj, Nicholas; Shibata, Yoko
  • Nature, Vol. 490, Issue 7421
  • DOI: 10.1038/nature11558

The Bombesin Receptor Subtypes Have Distinct G Protein Specificities
journal, April 1999

  • Jian, Xiaoying; Sainz, Eduardo; Clark, William A.
  • Journal of Biological Chemistry, Vol. 274, Issue 17
  • DOI: 10.1074/jbc.274.17.11573

Energy Landscapes as a Tool to Integrate GPCR Structure, Dynamics, and Function
journal, October 2010

How good are my data and what is the resolution?
journal, June 2013

  • Evans, Philip R.; Murshudov, Garib N.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 69, Issue 7
  • DOI: 10.1107/S0907444913000061

Structural Basis for Allosteric Regulation of GPCRs by Sodium Ions
journal, July 2012

Structure of signaling-competent neurotensin receptor 1 obtained by directed evolution in Escherichia coli
journal, January 2014

  • Egloff, Pascal; Hillenbrand, Matthias; Klenk, Christoph
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 6
  • DOI: 10.1073/pnas.1317903111

Structure and functional expression of the cloned rat neurotensin receptor
journal, June 1990

Lipidic cubic phases: A novel concept for the crystallization of membrane proteins
journal, December 1996

  • Landau, E. M.; Rosenbusch, J. P.
  • Proceedings of the National Academy of Sciences, Vol. 93, Issue 25
  • DOI: 10.1073/pnas.93.25.14532

Rastering strategy for screening and centring of microcrystal samples of human membrane proteins with a sub-10 µm size X-ray synchrotron beam
journal, June 2009

  • Cherezov, Vadim; Hanson, Michael A.; Griffith, Mark T.
  • Journal of The Royal Society Interface, Vol. 6, Issue suppl_5
  • DOI: 10.1098/rsif.2009.0142.focus

High-resolution crystal structure of human protease-activated receptor 1
journal, December 2012

  • Zhang, Cheng; Srinivasan, Yoga; Arlow, Daniel H.
  • Nature, Vol. 492, Issue 7429
  • DOI: 10.1038/nature11701

Functional Reconstitution in Situ of 5-Hydroxytryptamine 2c (5HT 2c ) Receptors with α q and Inverse Agonism of 5HT 2c Receptor Antagonists
journal, September 1996

Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity
text, January 2008

  • A., Sarkar, C.; I., Dodevski,; M., Kenig,
  • National Academy of Sciences
  • DOI: 10.5167/uzh-4809

Rhodopsin mutants that bind but fail to activate transducin
journal, October 1990

Rhodopsin mutants that bind but fail to activate transducin
journal, October 1990

Structure of a nanobody-stabilized active state of the β2 adrenoceptor
journal, January 2011

  • Rasmussen, Søren G. F.; Choi, Hee-Jung; Fung, Juan Jose
  • Nature, Vol. 469, Issue 7329
  • DOI: 10.1038/nature09648

High-Resolution Crystal Structure of an Engineered Human  2-Adrenergic G Protein-Coupled Receptor
journal, October 2007

Crystallizing membrane proteins using lipidic mesophases
journal, April 2009

Use of TLS parameters to model anisotropic displacements in macromolecular refinement
journal, January 2001

  • Winn, M. D.; Isupov, M. N.; Murshudov, G. N.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 57, Issue 1
  • DOI: 10.1107/S0907444900014736

Crystal structure of the β2 adrenergic receptor–Gs protein complex
journal, July 2011

  • Rasmussen, Søren G. F.; DeVree, Brian T.; Zou, Yaozhong
  • Nature, Vol. 477, Issue 7366
  • DOI: 10.1038/nature10361

Features and development of Coot
journal, March 2010

  • Emsley, P.; Lohkamp, B.; Scott, W. G.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4
  • DOI: 10.1107/S0907444910007493

A crystal clear solution for determining G-protein-coupled receptor structures
journal, September 2012

Maltose–neopentyl glycol (MNG) amphiphiles for solubilization, stabilization and crystallization of membrane proteins
journal, October 2010

  • Chae, Pil Seok; Rasmussen, Søren G. F.; Rana, Rohini R.
  • Nature Methods, Vol. 7, Issue 12
  • DOI: 10.1038/nmeth.1526

Biochemical and pharmacological profile of a potent and selective nonpeptide antagonist of the neurotensin receptor.
journal, January 1993

  • Gully, D.; Canton, M.; Boigegrain, R.
  • Proceedings of the National Academy of Sciences, Vol. 90, Issue 1
  • DOI: 10.1073/pnas.90.1.65

The Highly Conserved DRY Motif of Class A G Protein-Coupled Receptors: Beyond the Ground State
journal, December 2006

  • Rovati, G. Enrico; Capra, Valérie; Neubig, Richard R.
  • Molecular Pharmacology, Vol. 71, Issue 4
  • DOI: 10.1124/mol.106.029470

Neuropeptide receptor ligands as drugs for psychiatric diseases: the end of the beginning?
journal, May 2012

  • Griebel, Guy; Holsboer, Florian
  • Nature Reviews Drug Discovery, Vol. 11, Issue 6
  • DOI: 10.1038/nrd3702

Molecular control of δ-opioid receptor signalling
journal, January 2014

  • Fenalti, Gustavo; Giguere, Patrick M.; Katritch, Vsevolod
  • Nature, Vol. 506, Issue 7487
  • DOI: 10.1038/nature12944

Pivotal Role of an Aspartate Residue in Sodium Sensitivity and Coupling to G Proteins of Neurotensin Receptors
journal, February 1999

  • Martin, Stéphane; Botto, Jean-Marie; Vincent, Jean-Pierre
  • Molecular Pharmacology, Vol. 55, Issue 2
  • DOI: 10.1124/mol.55.2.210

Modulation of the Interaction between Neurotensin Receptor NTS1 and Gq Protein by Lipid
journal, March 2012

  • Inagaki, Sayaka; Ghirlando, Rodolfo; White, Jim F.
  • Journal of Molecular Biology, Vol. 417, Issue 1-2
  • DOI: 10.1016/j.jmb.2012.01.023

Crystal structure of metarhodopsin II
journal, March 2011

  • Choe, Hui-Woog; Kim, Yong Ju; Park, Jung Hee
  • Nature, Vol. 471, Issue 7340
  • DOI: 10.1038/nature09789

Dependence of critical micelle concentration of a zwitterionic detergent on ionic strength: implications in receptor solubilization
journal, August 1996

Structural Basis for Allosteric Regulation of GPCRs by Sodium Ions
journal, July 2012

Activation and allosteric modulation of a muscarinic acetylcholine receptor
journal, November 2013

  • Kruse, Andrew C.; Ring, Aaron M.; Manglik, Aashish
  • Nature, Vol. 504, Issue 7478
  • DOI: 10.1038/nature12735

The Isolation of a New Hypotensive Peptide, Neurotensin, from Bovine Hypothalami
journal, October 1973

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    • DOI: 10.1038/nature18324

    A mutagenesis and screening strategy to generate optimally thermostabilized membrane proteins for structural studies
    journal, July 2016

    • Magnani, Francesca; Serrano-Vega, Maria J.; Shibata, Yoko
    • Nature Protocols, Vol. 11, Issue 8
    • DOI: 10.1038/nprot.2016.088
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