Enzyme Induced Formation of Monodisperse Hydrogel Nanoparticles Tunable in Size

Bocharova, Vera; Sharp, Danna; Jones, Aaron; Cheng, Shiwang; Griffin, Philip J.; Agapov, Alexander L.; Voylov, Dmitry; Wang, Yangyang; Kisliuk, Alexander; Melman, Artem; Sokolov, Alexei P.

doi:10.1021/acs.chemmater.5b00187

Title: Enzyme Induced Formation of Monodisperse Hydrogel Nanoparticles Tunable in Size

Abstract

Here, we report a novel approach to synthesize monodisperse hydrogel nanoparticles that are tunable in size. The distinctive feature of our approach is the use of a multicopper oxidase enzyme, laccase, as both a biocatalyst and template for nanoparticle growth. We utilize the ferroxidase activity of laccase to initiate localized production of iron(III) cations from the oxidation of iron(II) cations. We demonstrate that nanoparticles are formed in a dilute polymer solution of alginate as a result of cross-linking between alginate and enzymatically produced iron(III) cations. Exerting control over the enzymatic reaction allows for nanometer-scale tuning of the hydrogel nanoparticle radii in the range of 30–100 nm. Moreover, the nanoparticles and their growth kinetics were characterized via dynamic light scattering, atomic force microscopy, and UV–vis spectroscopy. Our finding opens up a new avenue for the synthesis of tunable nanoscale hydrogel particles for biomedical applications.

Authors:

Bocharova, Vera ^[1]; Sharp, Danna ^[2]; Jones, Aaron ^[2]; Cheng, Shiwang ^[1]; Griffin, Philip J. ^[3]; Agapov, Alexander L. ^[2]; Voylov, Dmitry ^[2]; Wang, Yangyang ^[1]; Kisliuk, Alexander ^[1]; Melman, Artem ^[4]; Sokolov, Alexei P. ^[5]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Univ. of Tennessee, Knoxville, TN (United States)
Univ. of Pennsylvania, Philadelphia, PA (United States)
Clarkson Univ., Potsdam, NY (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)

Publication Date:: Mon Mar 09 00:00:00 EDT 2015

Research Org.:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences (CNMS)

Sponsoring Org.:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

OSTI Identifier:: 1185968

Grant/Contract Number:: AC05-00OR22725; DMR-1408811

Resource Type:: Accepted Manuscript

Journal Name:: Chemistry of Materials

Additional Journal Information:: Journal Volume: 27; Journal Issue: 7; Journal ID: ISSN 0897-4756

Publisher:: American Chemical Society (ACS)

Country of Publication:: United States

Language:: English

Subject:: 36 MATERIALS SCIENCE

Citation Formats


                    Bocharova, Vera, Sharp, Danna, Jones, Aaron, Cheng, Shiwang, Griffin, Philip J., Agapov, Alexander L., Voylov, Dmitry, Wang, Yangyang, Kisliuk, Alexander, Melman, Artem, and Sokolov, Alexei P. Enzyme Induced Formation of Monodisperse Hydrogel Nanoparticles Tunable in Size.  United States: N. p., 2015. 
Web.  doi:10.1021/acs.chemmater.5b00187.

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                    Bocharova, Vera, Sharp, Danna, Jones, Aaron, Cheng, Shiwang, Griffin, Philip J., Agapov, Alexander L., Voylov, Dmitry, Wang, Yangyang, Kisliuk, Alexander, Melman, Artem, & Sokolov, Alexei P. Enzyme Induced Formation of Monodisperse Hydrogel Nanoparticles Tunable in Size.  United States.  https://doi.org/10.1021/acs.chemmater.5b00187

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                    Bocharova, Vera, Sharp, Danna, Jones, Aaron, Cheng, Shiwang, Griffin, Philip J., Agapov, Alexander L., Voylov, Dmitry, Wang, Yangyang, Kisliuk, Alexander, Melman, Artem, and Sokolov, Alexei P. Mon .  
"Enzyme Induced Formation of Monodisperse Hydrogel Nanoparticles Tunable in Size".  United States.  https://doi.org/10.1021/acs.chemmater.5b00187.  https://www.osti.gov/servlets/purl/1185968.

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@article{osti_1185968,

  title        = {Enzyme Induced Formation of Monodisperse Hydrogel Nanoparticles Tunable in Size},

  author       = {Bocharova, Vera and Sharp, Danna and Jones, Aaron and Cheng, Shiwang and Griffin, Philip J. and Agapov, Alexander L. and Voylov, Dmitry and Wang, Yangyang and Kisliuk, Alexander and Melman, Artem and Sokolov, Alexei P.},

  abstractNote = {Here, we report a novel approach to synthesize monodisperse hydrogel nanoparticles that are tunable in size. The distinctive feature of our approach is the use of a multicopper oxidase enzyme, laccase, as both a biocatalyst and template for nanoparticle growth. We utilize the ferroxidase activity of laccase to initiate localized production of iron(III) cations from the oxidation of iron(II) cations. We demonstrate that nanoparticles are formed in a dilute polymer solution of alginate as a result of cross-linking between alginate and enzymatically produced iron(III) cations. Exerting control over the enzymatic reaction allows for nanometer-scale tuning of the hydrogel nanoparticle radii in the range of 30–100 nm. Moreover, the nanoparticles and their growth kinetics were characterized via dynamic light scattering, atomic force microscopy, and UV–vis spectroscopy. Our finding opens up a new avenue for the synthesis of tunable nanoscale hydrogel particles for biomedical applications.},

  doi          = {10.1021/acs.chemmater.5b00187},

  journal      = {Chemistry of Materials},

  number       = 7,

  volume       = 27,

  place        = {United States},

  year         = {Mon Mar 09 00:00:00 EDT 2015},

  month        = {Mon Mar 09 00:00:00 EDT 2015}

}

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https://doi.org/10.1021/acs.chemmater.5b00187

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Title: Enzyme Induced Formation of Monodisperse Hydrogel Nanoparticles Tunable in Size

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Self-Assembled Nanogel of Hydrophobized Dendritic Dextrin for Protein Delivery
journal, July 2009

Biodegradable Nanogel Formation of Polylactide-Grafted Dextran Copolymer in Dilute Aqueous Solution and Enhancement of Its Stability by Stereocomplexation
journal, July 2007

Self-Cross-Linked Polymer Nanogels: A Versatile Nanoscopic Drug Delivery Platform
journal, December 2010

Surfactant-Free Synthesis of Biodegradable, Biocompatible, and Stimuli-Responsive Cationic Nanogel Particles
journal, September 2013

Release dynamics of ciprofloxacin from swellable nanocarriers of poly(2-hydroxyethyl methacrylate): an in vitro study
journal, June 2010

pH sensitive swelling and releasing behavior of nano-gels based on polyaspartamide graft copolymers
journal, April 2011

Lidocaine loaded biodegradable nanospheres
journal, August 1999

Synthesis and Characterizations of In Situ Cross-Linkable Gelatin and 4-Arm-PPO-PEO Hybrid Hydrogels via Enzymatic Reaction for Tissue Regenerative Medicine
journal, February 2012

Enzyme-mediated fast in situ formation of hydrogels from dextran–tyramine conjugates
journal, January 2007

Tailorable Cell Culture Platforms from Enzymatically Cross-Linked Multifunctional Poly(ethylene glycol)-Based Hydrogels
journal, January 2013

Enzymatically Crosslinked Dextran-Tyramine Hydrogels as Injectable Scaffolds for Cartilage Tissue Engineering
journal, August 2010

In Situ Forming Hydrogels Based on Tyramine Conjugated 4-Arm-PPO-PEO via Enzymatic Oxidative Reaction
journal, February 2010

Multicopper oxidase-1 is a ferroxidase essential for iron homeostasis in Drosophila melanogaster
journal, July 2012

Multi-Copper Oxidases and Human Iron Metabolism
journal, June 2013

Electrochemically Controlled Drug-Mimicking Protein Release from Iron-Alginate Thin-Films Associated with an Electrode
journal, January 2012

Electrochemically stimulated release of lysozyme from an alginate matrix cross-linked with iron cations
journal, January 2012

Photodegradable Iron(III) Cross-Linked Alginate Gels
journal, July 2012

A Study of the Chain Stiffness and Extension of Alginates, in Vitro Epimerized Alginates, and Periodate-Oxidized Alginates Using Size-Exclusion Chromatography Combined with Light Scattering and Viscosity Detectors
journal, July 2006

Dioxygen Reactivity of Laccase: Dependence on Laccase Source, pH, and Anion Inhibition
journal, January 2001

Preparation of Ionically Cross-Linked Pectin Nanoparticles in the Presence of Chlorides of Divalent and Monovalent Cations
journal, September 2013

Enzyme Kinetics
book, January 2008

Enzymatically Cross-Linked Hyperbranched Polyglycerol Hydrogels as Scaffolds for Living Cells
journal, October 2014

Crystal Structure of a Laccase from the Fungus Trametes versicolor at 1.90-Å Resolution Containing a Full Complement of Coppers
journal, August 2002

Isolation and structural analysis of the laccase gene from the ligninegrading fungus Phlebia radiata
journal, July 1991

Characterization and structural analysis of the laccase I gene from the newly isolated ligninolytic basidiomycete PM1 (CECT 2971)
journal, December 1993

Effect of Ca ²⁺ , Ba ²⁺ , and Sr ²⁺ on Alginate Microbeads
journal, April 2006

Reexamining the Egg-Box Model in Calcium−Alginate Gels with X-ray Diffraction
journal, February 2007

Oxidoreductase-Initiated Radical Polymerizations to Design Hydrogels and Micro/Nanogels: Mechanism, Molding, and Applications
journal, March 2018