DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Production of deuterated switchgrass by hydroponic cultivation

Abstract

Deuterium enrichment of biological materials can potential enable expanded experimental use of small angle neutron scattering (SANS) to investigate molecular structural transitions of complex systems such as plant cell walls. Two key advances have been made that facilitate cultivation of switchgrass, an important forage and biofuel crop, for controlled isotopic enrichment: (1) perfusion system with individual chambers and (2) hydroponic growth from tiller cuttings. Plants were grown and maintained for several months with periodic harvest. Photosynthetic activity was monitored by measurement of CO2 in outflow from the growth chambers. Plant morphology and composition appeared normal compared to matched controls grown with H2O. Using this improved method, gram quantities of switchgrass leaves and stems were produced by continuous hydroponic cultivation using growth medium consisting of basal mineral salts in 50% D2O. Deuterium incorporation was confirmed by detection of the O-D and C-D stretching peaks with FTIR and quantified by 1H- and 2H-NMR. Lastly, this capability to produce deuterated lignocellulosic biomass under controlled conditions will enhance investigation of cell wall structure and its deconstruction by neutron scattering and NMR techniques.

Authors:
 [1];  [2];  [3];  [4];  [1];  [1];  [1];  [5];  [6];  [1]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. Georgia Institute of Technology, Atlanta, GA (United States)
  3. Georgia Institute of Technology, Atlanta, GA (United States); Washington Univ., St. Louis, MO (United States)
  4. Georgia Institute of Technology, Atlanta, GA (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
  5. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Massachusetts, Amherst, MA (United States)
  6. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
Publication Date:
Research Org.:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1185899
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Planta
Additional Journal Information:
Journal Volume: 242; Journal Issue: 1; Journal ID: ISSN 0032-0935
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; biomass; switchgrass; D2O; deuteration; NMR; FTIR

Citation Formats

Evans, Barbara R., Bali, Garima, Foston, Marcus B., Ragauskas, Arthur J., O'Neill, Hugh Michael, Shah, Riddhi S., McGaughey, Joseph, Reeves, David T., Rempe, Caroline S., and Davison, Brian H. Production of deuterated switchgrass by hydroponic cultivation. United States: N. p., 2015. Web. doi:10.1007/s00425-015-2298-0.
Evans, Barbara R., Bali, Garima, Foston, Marcus B., Ragauskas, Arthur J., O'Neill, Hugh Michael, Shah, Riddhi S., McGaughey, Joseph, Reeves, David T., Rempe, Caroline S., & Davison, Brian H. Production of deuterated switchgrass by hydroponic cultivation. United States. https://doi.org/10.1007/s00425-015-2298-0
Evans, Barbara R., Bali, Garima, Foston, Marcus B., Ragauskas, Arthur J., O'Neill, Hugh Michael, Shah, Riddhi S., McGaughey, Joseph, Reeves, David T., Rempe, Caroline S., and Davison, Brian H. Tue . "Production of deuterated switchgrass by hydroponic cultivation". United States. https://doi.org/10.1007/s00425-015-2298-0. https://www.osti.gov/servlets/purl/1185899.
@article{osti_1185899,
title = {Production of deuterated switchgrass by hydroponic cultivation},
author = {Evans, Barbara R. and Bali, Garima and Foston, Marcus B. and Ragauskas, Arthur J. and O'Neill, Hugh Michael and Shah, Riddhi S. and McGaughey, Joseph and Reeves, David T. and Rempe, Caroline S. and Davison, Brian H.},
abstractNote = {Deuterium enrichment of biological materials can potential enable expanded experimental use of small angle neutron scattering (SANS) to investigate molecular structural transitions of complex systems such as plant cell walls. Two key advances have been made that facilitate cultivation of switchgrass, an important forage and biofuel crop, for controlled isotopic enrichment: (1) perfusion system with individual chambers and (2) hydroponic growth from tiller cuttings. Plants were grown and maintained for several months with periodic harvest. Photosynthetic activity was monitored by measurement of CO2 in outflow from the growth chambers. Plant morphology and composition appeared normal compared to matched controls grown with H2O. Using this improved method, gram quantities of switchgrass leaves and stems were produced by continuous hydroponic cultivation using growth medium consisting of basal mineral salts in 50% D2O. Deuterium incorporation was confirmed by detection of the O-D and C-D stretching peaks with FTIR and quantified by 1H- and 2H-NMR. Lastly, this capability to produce deuterated lignocellulosic biomass under controlled conditions will enhance investigation of cell wall structure and its deconstruction by neutron scattering and NMR techniques.},
doi = {10.1007/s00425-015-2298-0},
journal = {Planta},
number = 1,
volume = 242,
place = {United States},
year = {Tue Apr 21 00:00:00 EDT 2015},
month = {Tue Apr 21 00:00:00 EDT 2015}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 10 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

The effect of deuteration on the structure of bacterial cellulose
journal, June 2013


Adaptation and growth response of Arabidopsis thaliana to deuterium
journal, January 1968


Molecular breeding of switchgrass for use as a biofuel crop
journal, December 2007


Switchgrass as an energy crop for biofuel production: A review of its ligno-cellulosic chemical properties
journal, January 2010

  • David, Kasi; Ragauskas, Arthur J.
  • Energy & Environmental Science, Vol. 3, Issue 9
  • DOI: 10.1039/b926617h

Effect of D 2 O on Growth Properties and Chemical Structure of Annual Ryegrass ( Lolium multiflorum )
journal, March 2014

  • Evans, Barbara R.; Bali, Garima; Reeves, David T.
  • Journal of Agricultural and Food Chemistry, Vol. 62, Issue 12
  • DOI: 10.1021/jf4055566

Changes in lignocellulosic supramolecular and ultrastructure during dilute acid pretreatment of Populus and switchgrass
journal, December 2010


Biomass Characterization: Recent Progress in Understanding Biomass Recalcitrance
journal, August 2012

  • Foston, Marcus; Ragauskas, Arthur J.
  • Industrial Biotechnology, Vol. 8, Issue 4
  • DOI: 10.1089/ind.2012.0015

Deuterium incorporation in biomass cell wall components by NMR analysis
journal, January 2012

  • Foston, Marcus B.; McGaughey, Joseph; O'Neill, Hugh
  • The Analyst, Vol. 137, Issue 5
  • DOI: 10.1039/c2an16025k

Deuterated Organisms: Cultivation and Uses
journal, March 1966


Neutron Technologies for Bioenergy Research
journal, August 2012

  • Langan, Paul; Evans, Barbara R.; Foston, Marcus
  • Industrial Biotechnology, Vol. 8, Issue 4
  • DOI: 10.1089/ind.2012.0012

The Biology of Heavy Water
journal, February 1934


Breakdown of Cell Wall Nanostructure in Dilute Acid Pretreated Biomass
journal, September 2010

  • Pingali, Sai Venkatesh; Urban, Volker S.; Heller, William T.
  • Biomacromolecules, Vol. 11, Issue 9
  • DOI: 10.1021/bm100455h

Growth of Roots in Deuterium Oxide
journal, July 1937

  • Pratt, Robertson; Curry, James
  • American Journal of Botany, Vol. 24, Issue 7
  • DOI: 10.2307/2436423

Structure Determination of Partially Deuterated Carotenoids from Intrinsically Labeled Vegetables by HPLC-MS and 1 H NMR
journal, February 2005

  • Putzbach, Karsten; Krucker, Manfred; Albert, Klaus
  • Journal of Agricultural and Food Chemistry, Vol. 53, Issue 3
  • DOI: 10.1021/jf0487506

Transport of calcium, germanium and rubidium lons in rice seedlings in deuterium oxide.
journal, January 1984


Hydrogen isotope effect on transport of potassium ion in rice seedlings equilibrated with deuterium oxide.
journal, January 1984


Water and potassium ion absorption by deuterium oxide resistant winter rye seedlings.
journal, January 1985


Germination and Seedling Growth of Winter Rye in Deuterium Oxide
journal, March 1964

  • Siegel, S. M.; Halpern, L. A.; Giumarro, C.
  • Nature, Vol. 201, Issue 4925
  • DOI: 10.1038/2011244a0

Golden Rice is an effective source of vitamin A
journal, April 2009

  • Tang, Guangwen; Qin, Jian; Dolnikowski, Gregory G.
  • The American Journal of Clinical Nutrition, Vol. 89, Issue 6
  • DOI: 10.3945/ajcn.2008.27119

Works referencing / citing this record:

Production of deuterated biomass by cultivation of Lemna minor (duckweed) in D2O
journal, January 2019


Ultrastructure and Enzymatic Hydrolysis of Deuterated Switchgrass
journal, September 2018