Production of deuterated switchgrass by hydroponic cultivation
Abstract
Deuterium enrichment of biological materials can potential enable expanded experimental use of small angle neutron scattering (SANS) to investigate molecular structural transitions of complex systems such as plant cell walls. Two key advances have been made that facilitate cultivation of switchgrass, an important forage and biofuel crop, for controlled isotopic enrichment: (1) perfusion system with individual chambers and (2) hydroponic growth from tiller cuttings. Plants were grown and maintained for several months with periodic harvest. Photosynthetic activity was monitored by measurement of CO2 in outflow from the growth chambers. Plant morphology and composition appeared normal compared to matched controls grown with H2O. Using this improved method, gram quantities of switchgrass leaves and stems were produced by continuous hydroponic cultivation using growth medium consisting of basal mineral salts in 50% D2O. Deuterium incorporation was confirmed by detection of the O-D and C-D stretching peaks with FTIR and quantified by 1H- and 2H-NMR. Lastly, this capability to produce deuterated lignocellulosic biomass under controlled conditions will enhance investigation of cell wall structure and its deconstruction by neutron scattering and NMR techniques.
- Authors:
-
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Georgia Institute of Technology, Atlanta, GA (United States)
- Georgia Institute of Technology, Atlanta, GA (United States); Washington Univ., St. Louis, MO (United States)
- Georgia Institute of Technology, Atlanta, GA (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Massachusetts, Amherst, MA (United States)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1185899
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Planta
- Additional Journal Information:
- Journal Volume: 242; Journal Issue: 1; Journal ID: ISSN 0032-0935
- Publisher:
- Springer
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; biomass; switchgrass; D2O; deuteration; NMR; FTIR
Citation Formats
Evans, Barbara R., Bali, Garima, Foston, Marcus B., Ragauskas, Arthur J., O'Neill, Hugh Michael, Shah, Riddhi S., McGaughey, Joseph, Reeves, David T., Rempe, Caroline S., and Davison, Brian H. Production of deuterated switchgrass by hydroponic cultivation. United States: N. p., 2015.
Web. doi:10.1007/s00425-015-2298-0.
Evans, Barbara R., Bali, Garima, Foston, Marcus B., Ragauskas, Arthur J., O'Neill, Hugh Michael, Shah, Riddhi S., McGaughey, Joseph, Reeves, David T., Rempe, Caroline S., & Davison, Brian H. Production of deuterated switchgrass by hydroponic cultivation. United States. https://doi.org/10.1007/s00425-015-2298-0
Evans, Barbara R., Bali, Garima, Foston, Marcus B., Ragauskas, Arthur J., O'Neill, Hugh Michael, Shah, Riddhi S., McGaughey, Joseph, Reeves, David T., Rempe, Caroline S., and Davison, Brian H. Tue .
"Production of deuterated switchgrass by hydroponic cultivation". United States. https://doi.org/10.1007/s00425-015-2298-0. https://www.osti.gov/servlets/purl/1185899.
@article{osti_1185899,
title = {Production of deuterated switchgrass by hydroponic cultivation},
author = {Evans, Barbara R. and Bali, Garima and Foston, Marcus B. and Ragauskas, Arthur J. and O'Neill, Hugh Michael and Shah, Riddhi S. and McGaughey, Joseph and Reeves, David T. and Rempe, Caroline S. and Davison, Brian H.},
abstractNote = {Deuterium enrichment of biological materials can potential enable expanded experimental use of small angle neutron scattering (SANS) to investigate molecular structural transitions of complex systems such as plant cell walls. Two key advances have been made that facilitate cultivation of switchgrass, an important forage and biofuel crop, for controlled isotopic enrichment: (1) perfusion system with individual chambers and (2) hydroponic growth from tiller cuttings. Plants were grown and maintained for several months with periodic harvest. Photosynthetic activity was monitored by measurement of CO2 in outflow from the growth chambers. Plant morphology and composition appeared normal compared to matched controls grown with H2O. Using this improved method, gram quantities of switchgrass leaves and stems were produced by continuous hydroponic cultivation using growth medium consisting of basal mineral salts in 50% D2O. Deuterium incorporation was confirmed by detection of the O-D and C-D stretching peaks with FTIR and quantified by 1H- and 2H-NMR. Lastly, this capability to produce deuterated lignocellulosic biomass under controlled conditions will enhance investigation of cell wall structure and its deconstruction by neutron scattering and NMR techniques.},
doi = {10.1007/s00425-015-2298-0},
journal = {Planta},
number = 1,
volume = 242,
place = {United States},
year = {Tue Apr 21 00:00:00 EDT 2015},
month = {Tue Apr 21 00:00:00 EDT 2015}
}
Web of Science
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Works referencing / citing this record:
Production of deuterated biomass by cultivation of Lemna minor (duckweed) in D2O
journal, January 2019
- Evans, Barbara R.; Foston, Marcus; O’Neill, Hugh M.
- Planta, Vol. 249, Issue 5
Ultrastructure and Enzymatic Hydrolysis of Deuterated Switchgrass
journal, September 2018
- Bhagia, Samarthya; Meng, Xianzhi; Evans, Barbara R.
- Scientific Reports, Vol. 8, Issue 1