Synechococcus elongatus UTEX 2973, a fast growing cyanobacterial chassis for biosynthesis using light and CO2
Abstract
Photosynthetic microbes are of emerging interest as production organisms in biotechnology because they can grow autotrophically using sunlight, an abundant energy source, and CO2, a greenhouse gas. Important traits for such microbes are fast growth and amenability to genetic manipulation. Here we describe Synechococcus elongatus UTEX 2973, a unicellular cyanobacterium capable of rapid autotrophic growth, comparable to heterotrophic industrial hosts such as yeast. Synechococcus 2973 can be readily transformed for facile generation of desired knockout and knock-in mutations. Genome sequencing coupled with global proteomics studies revealed that Synechococcus 2973 is a close relative of the widely studied cyanobacterium Synechococcus elongatus PCC 7942, an organism that grows more than two times slower. A small number of nucleotide changes are the only significant differences between the genomes of these two cyanobacterial strains. Thus, our study has unraveled genetic determinants necessary for rapid growth of cyanobacterial strains of significant industrial potential.
- Authors:
- Publication Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL); Energy Frontier Research Centers (EFRC) (United States). Photosynthetic Antenna Research Center (PARC)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1171276
- Report Number(s):
- PNNL-SA-105375
Journal ID: ISSN 2045-2322; srep08132
- Grant/Contract Number:
- AC05-76RL01830; SC 0001035
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Scientific Reports
- Additional Journal Information:
- Journal Volume: 5; Journal ID: ISSN 2045-2322
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS; Environmental Molecular Sciences Laboratory
Citation Formats
Yu, Jingjie, Liberton, Michelle, Cliften, Paul F., Head, Richard D., Jacobs, Jon M., Smith, Richard D., Koppenaal, David W., Brand, Jerry J., and Pakrasi, Himadri B. Synechococcus elongatus UTEX 2973, a fast growing cyanobacterial chassis for biosynthesis using light and CO2. United States: N. p., 2015.
Web. doi:10.1038/srep08132.
Yu, Jingjie, Liberton, Michelle, Cliften, Paul F., Head, Richard D., Jacobs, Jon M., Smith, Richard D., Koppenaal, David W., Brand, Jerry J., & Pakrasi, Himadri B. Synechococcus elongatus UTEX 2973, a fast growing cyanobacterial chassis for biosynthesis using light and CO2. United States. https://doi.org/10.1038/srep08132
Yu, Jingjie, Liberton, Michelle, Cliften, Paul F., Head, Richard D., Jacobs, Jon M., Smith, Richard D., Koppenaal, David W., Brand, Jerry J., and Pakrasi, Himadri B. Fri .
"Synechococcus elongatus UTEX 2973, a fast growing cyanobacterial chassis for biosynthesis using light and CO2". United States. https://doi.org/10.1038/srep08132. https://www.osti.gov/servlets/purl/1171276.
@article{osti_1171276,
title = {Synechococcus elongatus UTEX 2973, a fast growing cyanobacterial chassis for biosynthesis using light and CO2},
author = {Yu, Jingjie and Liberton, Michelle and Cliften, Paul F. and Head, Richard D. and Jacobs, Jon M. and Smith, Richard D. and Koppenaal, David W. and Brand, Jerry J. and Pakrasi, Himadri B.},
abstractNote = {Photosynthetic microbes are of emerging interest as production organisms in biotechnology because they can grow autotrophically using sunlight, an abundant energy source, and CO2, a greenhouse gas. Important traits for such microbes are fast growth and amenability to genetic manipulation. Here we describe Synechococcus elongatus UTEX 2973, a unicellular cyanobacterium capable of rapid autotrophic growth, comparable to heterotrophic industrial hosts such as yeast. Synechococcus 2973 can be readily transformed for facile generation of desired knockout and knock-in mutations. Genome sequencing coupled with global proteomics studies revealed that Synechococcus 2973 is a close relative of the widely studied cyanobacterium Synechococcus elongatus PCC 7942, an organism that grows more than two times slower. A small number of nucleotide changes are the only significant differences between the genomes of these two cyanobacterial strains. Thus, our study has unraveled genetic determinants necessary for rapid growth of cyanobacterial strains of significant industrial potential.},
doi = {10.1038/srep08132},
journal = {Scientific Reports},
number = ,
volume = 5,
place = {United States},
year = {Fri Jan 30 00:00:00 EST 2015},
month = {Fri Jan 30 00:00:00 EST 2015}
}
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Works referenced in this record:
Fatty acid production in genetically modified cyanobacteria
journal, April 2011
- Liu, X.; Sheng, J.; Curtiss III, R.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 17, p. 6899-6904
A small polypeptide triggers complete degradation of light-harvesting phycobiliproteins in nutrient-deprived cyanobacteria.
journal, March 1994
- Collier, J. L.; Grossman, A. R.
- The EMBO Journal, Vol. 13, Issue 5
Unique Thylakoid Membrane Architecture of a Unicellular N 2 -Fixing Cyanobacterium Revealed by Electron Tomography
journal, December 2010
- Liberton, Michelle; Austin, Jotham R.; Berg, R. Howard
- Plant Physiology, Vol. 155, Issue 4
Efficient gene transfer in Synechococcus sp. strains PCC 7942 and PCC 6301 by interspecies conjugation and chromosomal recombination.
journal, November 1994
- Tsinoremas, N. F.; Kutach, A. K.; Strayer, C. A.
- Journal of Bacteriology, Vol. 176, Issue 21
A Carboxyl-terminal Cys 2 /His 2 -type Zinc-finger Motif in DNA Primase Influences DNA Content in Synechococcus PCC 7942
journal, August 1998
- Bird, Amanda J.; Turner-Cavet, Jennifer S.; Lakey, Jeremy H.
- Journal of Biological Chemistry, Vol. 273, Issue 33
Natural genetic transformation: prevalence, mechanisms and function
journal, December 2007
- Johnsborg, Ola; Eldholm, Vegard; Håvarstein, Leiv Sigve
- Research in Microbiology, Vol. 158, Issue 10, p. 767-778
Engineered cyanobacteria: Teaching an old bug new tricks
journal, May 2011
- Ruffing, Anne M.
- Bioengineered Bugs, Vol. 2, Issue 3
Microbial engineering for the production of advanced biofuels
journal, August 2012
- Peralta-Yahya, Pamela P.; Zhang, Fuzhong; del Cardayre, Stephen B.
- Nature, Vol. 488, Issue 7411
Chemically Etched Open Tubular and Monolithic Emitters for Nanoelectrospray Ionization Mass Spectrometry
journal, November 2006
- Kelly, Ryan T.; Page, Jason S.; Luo, Quanzhou
- Analytical Chemistry, Vol. 78, Issue 22, p. 7796-7801
Reversed-phase chromatography with multiple fraction concatenation strategy for proteome profiling of human MCF10A cells
journal, April 2011
- Wang, Yuexi; Yang, Feng; Gritsenko, Marina A.
- PROTEOMICS, Vol. 11, Issue 10
Metabolic engineering of cyanobacteria for 1-butanol production from carbon dioxide
journal, July 2011
- Lan, Ethan I.; Liao, James C.
- Metabolic Engineering, Vol. 13, Issue 4
Marine cyanobacteria—a prolific source of natural products
journal, November 2001
- Burja, Adam M.; Banaigs, Bernard; Abou-Mansour, Eliane
- Tetrahedron, Vol. 57, Issue 46
Production of Squalene in Synechocystis sp. PCC 6803
journal, March 2014
- Englund, Elias; Pattanaik, Bagmi; Ubhayasekera, Sarojini Jayantha K.
- PLoS ONE, Vol. 9, Issue 3
Insertional mutagenesis by random cloning of antibiotic resistance genes into the genome of the cyanobacterium Synechocystis strain PCC 6803.
journal, June 1989
- Labarre, J.; Chauvat, F.; Thuriaux, P.
- Journal of Bacteriology, Vol. 171, Issue 6
Proteome Analysis of Borrelia burgdorferi Response to Environmental Change
journal, November 2010
- Angel, Thomas E.; Luft, Benjamin J.; Yang, Xiaohua
- PLoS ONE, Vol. 5, Issue 11
Cyanobacterial conversion of carbon dioxide to 2,3-butanediol
journal, January 2013
- Oliver, John W. K.; Machado, Iara M. P.; Yoneda, Hisanari
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 4
Interactions of photosynthesis with genome size and function
journal, July 2013
- Raven, John A.; Beardall, John; Larkum, Anthony W. D.
- Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 368, Issue 1622
Proteome Analysis of Borrelia burgdorferi Response to Environmental Change
journal, November 2010
- Angel, Thomas E.; Luft, Benjamin J.; Yang, Xiaohua
- PLoS ONE, Vol. 5, Issue 11
Reduction of Photoautotrophic Productivity in the Cyanobacterium Synechocystis sp. Strain PCC 6803 by Phycobilisome Antenna Truncation
journal, June 2012
- Page, Lawrence E.; Liberton, Michelle; Pakrasi, Himadri B.
- Applied and Environmental Microbiology, Vol. 78, Issue 17
Liquid Chromatography-Mass Spectrometry-based Quantitative Proteomics
journal, June 2011
- Xie, Fang; Liu, Tao; Qian, Wei-Jun
- Journal of Biological Chemistry, Vol. 286, Issue 29
Probability-Based Evaluation of Peptide and Protein Identifications from Tandem Mass Spectrometry and SEQUEST Analysis: The Human Proteome
journal, February 2005
- Qian, Wei-Jun; Liu, Tao; Monroe, Matthew E.
- Journal of Proteome Research, Vol. 4, Issue 1
Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism
journal, January 2010
- Lindberg, Pia; Park, Sungsoon; Melis, Anastasios
- Metabolic Engineering, Vol. 12, Issue 1, p. 70-79
Reduction of conjugal transfer efficiency by three restriction activities of Anabaena sp. strain PCC 7120.
journal, March 1997
- Elhai, J.; Vepritskiy, A.; Muro-Pastor, A. M.
- Journal of Bacteriology, Vol. 179, Issue 6
A perspective: Photosynthetic production of fatty acid-based biofuels in genetically engineered cyanobacteria
journal, November 2010
- Lu, Xuefeng
- Biotechnology Advances, Vol. 28, Issue 6
Comparative proteomics of cell division mutants and wild-type of Synechococcus sp. strain PCC 7942
journal, August 2007
- Koksharova, O. A.; Klint, J.; Rasmussen, U.
- Microbiology, Vol. 153, Issue 8
Natural genetic transformation: prevalence, mechanisms and function
journal, December 2007
- Johnsborg, Ola; Eldholm, Vegard; Håvarstein, Leiv Sigve
- Research in Microbiology, Vol. 158, Issue 10, p. 767-778
Nitrate Transport and Not Photoinhibition Limits Growth of the Freshwater Cyanobacterium Synechococcus Species PCC 6301 at Low Temperature
journal, February 1999
- Sakamoto, Toshio; Bryant, Donald A.
- Plant Physiology, Vol. 119, Issue 2
Unique Thylakoid Membrane Architecture of a Unicellular N 2 -Fixing Cyanobacterium Revealed by Electron Tomography
journal, December 2010
- Liberton, Michelle; Austin, Jotham R.; Berg, R. Howard
- Plant Physiology, Vol. 155, Issue 4
Chemically Etched Open Tubular and Monolithic Emitters for Nanoelectrospray Ionization Mass Spectrometry
journal, November 2006
- Kelly, Ryan T.; Page, Jason S.; Luo, Quanzhou
- Analytical Chemistry, Vol. 78, Issue 22, p. 7796-7801
A small polypeptide triggers complete degradation of light-harvesting phycobiliproteins in nutrient-deprived cyanobacteria.
journal, March 1994
- Collier, J. L.; Grossman, A. R.
- The EMBO Journal, Vol. 13, Issue 5
Fatty acid production in genetically modified cyanobacteria
journal, April 2011
- Liu, X.; Sheng, J.; Curtiss III, R.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 17, p. 6899-6904
Simple Conditions for Growth of Unicellular Blue-Green Algae on Plates
journal, March 1968
- Allen, Mary Mennes
- Journal of Phycology, Vol. 4, Issue 1
Redirecting Reductant Flux into Hydrogen Production via Metabolic Engineering of Fermentative Carbon Metabolism in a Cyanobacterium
journal, June 2010
- McNeely, K.; Xu, Y.; Bennette, N.
- Applied and Environmental Microbiology, Vol. 76, Issue 15
Engineered cyanobacteria: Teaching an old bug new tricks
journal, May 2011
- Ruffing, Anne M.
- Bioengineered Bugs, Vol. 2, Issue 3
Tracking the Light Environment by Cyanobacteria and the Dynamic Nature of Light Harvesting
journal, February 2001
- Grossman, Arthur R.; Bhaya, Devaki; He, Qingfang
- Journal of Biological Chemistry, Vol. 276, Issue 15
Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology
journal, March 2010
- Huang, Hsin-Ho; Camsund, Daniel; Lindblad, Peter
- Nucleic Acids Research, Vol. 38, Issue 8
Insertional mutagenesis by random cloning of antibiotic resistance genes into the genome of the cyanobacterium Synechocystis strain PCC 6803.
journal, June 1989
- Labarre, J.; Chauvat, F.; Thuriaux, P.
- Journal of Bacteriology, Vol. 171, Issue 6
Production of Squalene in Synechocystis sp. PCC 6803
journal, March 2014
- Englund, Elias; Pattanaik, Bagmi; Ubhayasekera, Sarojini Jayantha K.
- PLoS ONE, Vol. 9, Issue 3
Microbial engineering for the production of advanced biofuels
journal, August 2012
- Peralta-Yahya, Pamela P.; Zhang, Fuzhong; del Cardayre, Stephen B.
- Nature, Vol. 488, Issue 7411
A perspective: Photosynthetic production of fatty acid-based biofuels in genetically engineered cyanobacteria
journal, November 2010
- Lu, Xuefeng
- Biotechnology Advances, Vol. 28, Issue 6
Carbon Dioxide Mitigation using Thermophilic Cyanobacteria
journal, January 2007
- Ono, E.; Cuello, J. L.
- Biosystems Engineering, Vol. 96, Issue 1
Probability-Based Evaluation of Peptide and Protein Identifications from Tandem Mass Spectrometry and SEQUEST Analysis: The Human Proteome
journal, February 2005
- Qian, Wei-Jun; Liu, Tao; Monroe, Matthew E.
- Journal of Proteome Research, Vol. 4, Issue 1
Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering
journal, June 2011
- Quintana, Naira; Van der Kooy, Frank; Van de Rhee, Miranda D.
- Applied Microbiology and Biotechnology, Vol. 91, Issue 3
A Carboxyl-terminal Cys 2 /His 2 -type Zinc-finger Motif in DNA Primase Influences DNA Content in Synechococcus PCC 7942
journal, August 1998
- Bird, Amanda J.; Turner-Cavet, Jennifer S.; Lakey, Jeremy H.
- Journal of Biological Chemistry, Vol. 273, Issue 33
Photobioreactor for cultivation and real-time, in-situ measurement of O 2 and CO 2 exchange rates, growth dynamics, and of chlorophyll fluorescence emission of photoautotrophic microorganisms
journal, June 2009
- Červený, Jan; Šetlík, Ivan; Trtílek, Martin
- Engineering in Life Sciences, Vol. 9, Issue 3
Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism
journal, January 2010
- Lindberg, Pia; Park, Sungsoon; Melis, Anastasios
- Metabolic Engineering, Vol. 12, Issue 1, p. 70-79
Complete nucleotide sequence of the freshwater unicellular cyanobacterium Synechococcus elongatus PCC 6301 chromosome: gene content and organization
journal, January 2007
- Sugita, Chieko; Ogata, Koretsugu; Shikata, Masamitsu
- Photosynthesis Research, Vol. 93, Issue 1-3
Dynamic Proteome Analysis of Cyanothece sp. ATCC 51142 under Constant Light
journal, December 2011
- Aryal, Uma K.; Stöckel, Jana; Welsh, Eric A.
- Journal of Proteome Research, Vol. 11, Issue 2
Nutrition and Growth of Several Blue-Green Algae
journal, March 1955
- Kratz, William A.; Myers, Jack
- American Journal of Botany, Vol. 42, Issue 3
Interactions of photosynthesis with genome size and function
journal, July 2013
- Raven, John A.; Beardall, John; Larkum, Anthony W. D.
- Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 368, Issue 1622
Fully Automated Four-Column Capillary LC−MS System for Maximizing Throughput in Proteomic Analyses
journal, January 2008
- Livesay, Eric A.; Tang, Keqi; Taylor, Beverley K.
- Analytical Chemistry, Vol. 80, Issue 1
Reduction of conjugal transfer efficiency by three restriction activities of Anabaena sp. strain PCC 7120.
journal, March 1997
- Elhai, J.; Vepritskiy, A.; Muro-Pastor, A. M.
- Journal of Bacteriology, Vol. 179, Issue 6
Nutrition and Growth of Several Blue-Green Algae
journal, March 1955
- Kratz, William A.; Myers, Jack
- American Journal of Botany, Vol. 42, Issue 3
Effect of iron deficiency and iron restoration on ultrastructure of Anacystis nidulans.
journal, January 1983
- Sherman, D. M.; Sherman, L. A.
- Journal of Bacteriology, Vol. 156, Issue 1
Spectral Probabilities and Generating Functions of Tandem Mass Spectra: A Strike against Decoy Databases
journal, August 2008
- Kim, Sangtae; Gupta, Nitin; Pevzner, Pavel A.
- Journal of Proteome Research, Vol. 7, Issue 8
Reversed-phase chromatography with multiple fraction concatenation strategy for proteome profiling of human MCF10A cells
journal, April 2011
- Wang, Yuexi; Yang, Feng; Gritsenko, Marina A.
- PROTEOMICS, Vol. 11, Issue 10
DNA-uptake in the naturally competent cyanobacterium, Synechocystis sp. PCC 6803
journal, June 1995
- Barten, Roland; Lill, Holger
- FEMS Microbiology Letters, Vol. 129, Issue 1
Works referencing / citing this record:
Identifying the Metabolic Differences of a Fast-Growth Phenotype in Synechococcus UTEX 2973
journal, January 2017
- Mueller, Thomas J.; Ungerer, Justin L.; Pakrasi, Himadri B.
- Scientific Reports, Vol. 7, Issue 1
A penalty on photosynthetic growth in fluctuating light
journal, October 2017
- Graham, Percival J.; Nguyen, Brian; Burdyny, Thomas
- Scientific Reports, Vol. 7, Issue 1
The international journeys and aliases of Synechococcus elongatus
journal, December 2018
- Golden, Susan S.
- New Zealand Journal of Botany, Vol. 57, Issue 2
Optimal proteome allocation strategies for phototrophic growth in a light-limited chemostat
journal, October 2019
- Faizi, Marjan; Steuer, Ralf
- Microbial Cell Factories, Vol. 18, Issue 1
Metabolic engineering of phosphite metabolism in Synechococcus elongatus PCC 7942 as an effective measure to control biological contaminants in outdoor raceway ponds
journal, July 2020
- González-Morales, Sandra Isabel; Pacheco-Gutiérrez, Navid Berenice; Ramírez-Rodríguez, Carlos A.
- Biotechnology for Biofuels, Vol. 13, Issue 1
Cyanobacteria-Based Bio-Oxygen Pump Promoting Hypoxia-Resistant Photodynamic Therapy
journal, March 2020
- Sun, Tao; Zhang, Yingying; Zhang, Chaonan
- Frontiers in Bioengineering and Biotechnology, Vol. 8
CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973
journal, June 2016
- Wendt, Kristen E.; Ungerer, Justin; Cobb, Ryan E.
- Microbial Cell Factories, Vol. 15, Issue 1
Development of a longevous two-species biophotovoltaics with constrained electron flow
journal, September 2019
- Zhu, Huawei; Meng, Hengkai; Zhang, Wei
- Nature Communications, Vol. 10, Issue 1
Elevated carbon dioxide levels lead to proteome-wide alterations for optimal growth of a fast-growing cyanobacterium, Synechococcus elongatus PCC 11801
journal, April 2019
- Mehta, Kanika; Jaiswal, Damini; Nayak, Monalisha
- Scientific Reports, Vol. 9, Issue 1
Carbon-free production of 2-deoxy- scyllo -inosose (DOI) in cyanobacterium Synechococcus elongatus PCC 7942
journal, January 2018
- Watanabe, Satoru; Ozawa, Hiroaki; Kato, Hiroaki
- Bioscience, Biotechnology, and Biochemistry, Vol. 82, Issue 1
From cyanochemicals to cyanofactories: a review and perspective
journal, January 2016
- Zhou, Jie; Zhu, Taicheng; Cai, Zhen
- Microbial Cell Factories, Vol. 15, Issue 1
Two-stage (photoautotrophy and heterotrophy) cultivation enables efficient production of bioplastic poly-3-hydroxybutyrate in auto-sedimenting cyanobacterium
journal, November 2016
- Monshupanee, Tanakarn; Nimdach, Palida; Incharoensakdi, Aran
- Scientific Reports, Vol. 6, Issue 1
PHB is Produced from Glycogen Turn-over during Nitrogen Starvation in Synechocystis sp. PCC 6803
journal, April 2019
- Koch, Moritz; Doello, Sofía; Gutekunst, Kirstin
- International Journal of Molecular Sciences, Vol. 20, Issue 8
CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973
journal, June 2016
- Wendt, Kristen E.; Ungerer, Justin; Cobb, Ryan E.
- Microbial Cell Factories, Vol. 15, Issue 1
Biogenic Weathering: Solubilization of Iron from Minerals by Epilithic Freshwater Algae and Cyanobacteria
journal, January 2018
- Mustoe, George
- Microorganisms, Vol. 6, Issue 1
Elevated carbon dioxide levels lead to proteome-wide alterations for optimal growth of a fast-growing cyanobacterium, Synechococcus elongatus PCC 11801
journal, April 2019
- Mehta, Kanika; Jaiswal, Damini; Nayak, Monalisha
- Scientific Reports, Vol. 9, Issue 1
A Novel Cyanobacterium Synechococcus elongatus PCC 11802 has Distinct Genomic and Metabolomic Characteristics Compared to its Neighbor PCC 11801
journal, January 2020
- Jaiswal, Damini; Sengupta, Annesha; Sengupta, Shinjinee
- Scientific Reports, Vol. 10, Issue 1
Engineering cyanobacteria for production of terpenoids
journal, November 2018
- Lin, Po-Cheng; Pakrasi, Himadri B.
- Planta, Vol. 249, Issue 1
New Applications of Synthetic Biology Tools for Cyanobacterial Metabolic Engineering
journal, February 2019
- Santos-Merino, María; Singh, Amit K.; Ducat, Daniel C.
- Frontiers in Bioengineering and Biotechnology, Vol. 7
Deciphering cyanobacterial phenotypes for fast photoautotrophic growth via isotopically nonstationary metabolic flux analysis
journal, November 2017
- Abernathy, Mary H.; Yu, Jingjie; Ma, Fangfang
- Biotechnology for Biofuels, Vol. 10, Issue 1
Microbial fuel cells (MFC) and microalgae; photo microbial fuel cell (PMFC) as complete recycling machines
journal, January 2019
- Greenman, John; Gajda, Iwona; Ieropoulos, Ioannis
- Sustainable Energy & Fuels, Vol. 3, Issue 10
Extremophile Microalgae: the potential for biotechnological application
journal, February 2020
- Malavasi, Veronica; Soru, Santina; Cao, Giacomo
- Journal of Phycology, Vol. 56, Issue 3
Erratum to: Is RAF1 protein from Synechocystis sp. PCC 6803 really needed in the cyanobacterial Rubisco assembly process?
journal, February 2017
- Kolesinski, Piotr; Rydzy, Malgorzata; Szczepaniak, Andrzej
- Photosynthesis Research, Vol. 132, Issue 2
Expression and secretion of a lytic polysaccharide monooxygenase by a fast-growing cyanobacterium
journal, April 2019
- Russo, D. A.; Zedler, J. A. Z.; Wittmann, D. N.
- Biotechnology for Biofuels, Vol. 12, Issue 1
Biogenic Weathering: Solubilization of Iron from Minerals by Epilithic Freshwater Algae and Cyanobacteria
journal, January 2018
- Mustoe, George
- Microorganisms, Vol. 6, Issue 1
A simple approach to spectrally resolved fluorescence and bright field microscopy over select regions of interest
journal, November 2016
- Dahlberg, Peter D.; Boughter, Christopher T.; Faruk, Nabil F.
- Review of Scientific Instruments, Vol. 87, Issue 11
Editorial: Cyanobacteria: The Green E. coli
journal, February 2016
- Ruffing, Anne M.; Kallas, Toivo
- Frontiers in Bioengineering and Biotechnology, Vol. 4
A Novel Cyanobacterium Synechococcus elongatus PCC 11802 has Distinct Genomic and Metabolomic Characteristics Compared to its Neighbor PCC 11801
journal, January 2020
- Jaiswal, Damini; Sengupta, Annesha; Sengupta, Shinjinee
- Scientific Reports, Vol. 10, Issue 1
Deciphering cyanobacterial phenotypes for fast photoautotrophic growth via isotopically nonstationary metabolic flux analysis
journal, November 2017
- Abernathy, Mary H.; Yu, Jingjie; Ma, Fangfang
- Biotechnology for Biofuels, Vol. 10, Issue 1
The potential of Synechococcus elongatus UTEX 2973 for sugar feedstock production
journal, April 2016
- Song, Kuo; Tan, Xiaoming; Liang, Yajing
- Applied Microbiology and Biotechnology, Vol. 100, Issue 18
Recent advances in synthetic biology of cyanobacteria
journal, May 2018
- Sengupta, Annesha; Pakrasi, Himadri B.; Wangikar, Pramod P.
- Applied Microbiology and Biotechnology, Vol. 102, Issue 13
Metabolic engineering of a fast-growing cyanobacterium Synechococcus elongatus PCC 11801 for photoautotrophic production of succinic acid
journal, May 2020
- Sengupta, Shinjinee; Jaiswal, Damini; Sengupta, Annesha
- Biotechnology for Biofuels, Vol. 13, Issue 1
In silico screening for candidate chassis strains of free fatty acid-producing cyanobacteria
journal, January 2017
- Motwalli, Olaa; Essack, Magbubah; Jankovic, Boris R.
- BMC Genomics, Vol. 18, Issue 1
Heterologous expression and biochemical characterisation of cyanotoxin biosynthesis pathways
journal, January 2019
- Cullen, Alescia; Pearson, Leanne A.; Mazmouz, Rabia
- Natural Product Reports, Vol. 36, Issue 8
Proteomic Insights into Phycobilisome Degradation, A Selective and Tightly Controlled Process in The Fast-Growing Cyanobacterium Synechococcus elongatus UTEX 2973
journal, August 2019
- Nagarajan, Aparna; Zhou, Mowei; Nguyen, Amelia Y.
- Biomolecules, Vol. 9, Issue 8
Physiological Significance of NAD Kinases in Cyanobacteria
journal, June 2019
- Ishikawa, Yuuma; Kawai-Yamada, Maki
- Frontiers in Plant Science, Vol. 10
Overexpression of bicarbonate transporters in the marine cyanobacterium Synechococcus sp. PCC 7002 increases growth rate and glycogen accumulation
journal, January 2020
- Gupta, Jai Kumar; Rai, Preeti; Jain, Kavish Kumar
- Biotechnology for Biofuels, Vol. 13, Issue 1
The Suitability of Orthogonal Hosts to Study Plant Cell Wall Biosynthesis
journal, November 2019
- Pauly, Markus; Gawenda, Niklas; Wagner, Christine
- Plants, Vol. 8, Issue 11
Unprecedented biomass and fatty acid production by the newly discovered cyanobacterium Synechococcus sp. PCC 11901
journal, June 2019
- Włodarczyk, Artur; Selão, Tiago Toscano; Norling, Birgitta
- Communications Biology
Comparative genomics reveals the molecular determinants of rapid growth of the cyanobacterium Synechococcus elongatus UTEX 2973
journal, November 2018
- Ungerer, Justin; Wendt, Kristen E.; Hendry, John I.
- Proceedings of the National Academy of Sciences, Vol. 115, Issue 50
Adjustments to Photosystem Stoichiometry and Electron Transfer Proteins Are Key to the Remarkably Fast Growth of the Cyanobacterium Synechococcus elongatus UTEX 2973
journal, February 2018
- Ungerer, Justin; Lin, Po-Cheng; Chen, Hui-Yuan
- mBio, Vol. 9, Issue 1
Emerging Species and Genome Editing Tools: Future Prospects in Cyanobacterial Synthetic Biology
journal, September 2019
- Gale, Grant A. R.; Schiavon Osorio, Alejandra A.; Mills, Lauren A.
- Microorganisms, Vol. 7, Issue 10
Progress and challenges in engineering cyanobacteria as chassis for light‐driven biotechnology
journal, August 2019
- Hitchcock, Andrew; Hunter, C. Neil; Canniffe, Daniel P.
- Microbial Biotechnology, Vol. 13, Issue 2
Progress and challenges in engineering cyanobacteria as chassis for light‐driven biotechnology
journal, August 2019
- Hitchcock, Andrew; Hunter, C. Neil; Canniffe, Daniel P.
- Microbial Biotechnology, Vol. 13, Issue 2
Diverse light responses of cyanobacteria mediated by phytochrome superfamily photoreceptors
journal, November 2018
- Wiltbank, Lisa B.; Kehoe, David M.
- Nature Reviews Microbiology, Vol. 17, Issue 1
Reply to Zhou and Li: Plasticity of the genomic haplotype of Synechococcus elongatus leads to rapid strain adaptation under laboratory conditions
journal, February 2019
- Ungerer, Justin; Wendt, Kristen E.; Hendry, John I.
- Proceedings of the National Academy of Sciences, Vol. 116, Issue 10
The primary transcriptome of the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973
journal, August 2018
- Tan, Xiaoming; Hou, Shengwei; Song, Kuo
- Biotechnology for Biofuels, Vol. 11, Issue 1
Physiological Significance of NAD Kinases in Cyanobacteria
journal, June 2019
- Ishikawa, Yuuma; Kawai-Yamada, Maki
- Frontiers in Plant Science, Vol. 10
Simulating cyanobacterial phenotypes by integrating flux balance analysis, kinetics, and a light distribution function
journal, December 2015
- He, Lian; Wu, Stephen G.; Wan, Ni
- Microbial Cell Factories, Vol. 14, Issue 1
The Suitability of Orthogonal Hosts to Study Plant Cell Wall Biosynthesis
journal, November 2019
- Pauly, Markus; Gawenda, Niklas; Wagner, Christine
- Plants, Vol. 8, Issue 11
A force awakens: exploiting solar energy beyond photosynthesis
journal, February 2019
- Russo, David A.; Zedler, Julie A. Z.; Jensen, Poul Erik
- Journal of Experimental Botany, Vol. 70, Issue 6
On the use of oxygenic photosynthesis for the sustainable production of commodity chemicals
journal, March 2019
- Pérez, Adam A.; Chen, Que; Hernández, Hugo Pineda
- Physiologia Plantarum, Vol. 166, Issue 1
Carbon recycling by cyanobacteria: improving CO2 fixation through chemical production
journal, August 2017
- Zhang, Angela; Carroll, Austin L.; Atsumi, Shota
- FEMS Microbiology Letters, Vol. 364, Issue 16
Emerging Species and Genome Editing Tools: Future Prospects in Cyanobacterial Synthetic Biology
journal, September 2019
- Gale, Grant A. R.; Schiavon Osorio, Alejandra A.; Mills, Lauren A.
- Microorganisms, Vol. 7, Issue 10
Cpf1 Is A Versatile Tool for CRISPR Genome Editing Across Diverse Species of Cyanobacteria
journal, December 2016
- Ungerer, Justin; Pakrasi, Himadri B.
- Scientific Reports, Vol. 6, Issue 1
Two-stage (photoautotrophy and heterotrophy) cultivation enables efficient production of bioplastic poly-3-hydroxybutyrate in auto-sedimenting cyanobacterium
journal, November 2016
- Monshupanee, Tanakarn; Nimdach, Palida; Incharoensakdi, Aran
- Scientific Reports, Vol. 6, Issue 1
Metabolic engineering of the pentose phosphate pathway for enhanced limonene production in the cyanobacterium Synechocysti s sp. PCC 6803
journal, December 2017
- Lin, Po-Cheng; Saha, Rajib; Zhang, Fuzhong
- Scientific Reports, Vol. 7, Issue 1
Oligomerization and characteristics of phosphoenolpyruvate carboxylase in Synechococcus PCC 7002
journal, February 2020
- Durall, Claudia; Kanchugal P., Sandesh; Selmer, Maria
- Scientific Reports, Vol. 10, Issue 1
Genome Features and Biochemical Characteristics of a Robust, Fast Growing and Naturally Transformable Cyanobacterium Synechococcus elongatus PCC 11801 Isolated from India
journal, November 2018
- Jaiswal, Damini; Sengupta, Annesha; Sohoni, Sujata
- Scientific Reports, Vol. 8, Issue 1
Expression and secretion of a lytic polysaccharide monooxygenase by a fast-growing cyanobacterium
journal, April 2019
- Russo, D. A.; Zedler, J. A. Z.; Wittmann, D. N.
- Biotechnology for Biofuels, Vol. 12, Issue 1
Construction and analysis of an artificial consortium based on the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 to produce the platform chemical 3-hydroxypropionic acid from CO2
journal, May 2020
- Zhang, Li; Chen, Lei; Diao, Jinjin
- Biotechnology for Biofuels, Vol. 13, Issue 1
Cellular trade-offs and optimal resource allocation during cyanobacterial diurnal growth
journal, July 2017
- Reimers, Alexandra-M.; Knoop, Henning; Bockmayr, Alexander
- Proceedings of the National Academy of Sciences, Vol. 114, Issue 31
Optimal proteome allocation strategies for phototrophic growth in a light-limited chemostat
journal, October 2019
- Faizi, Marjan; Steuer, Ralf
- Microbial Cell Factories, Vol. 18, Issue 1
Navigating the fitness landscape using multiallele genome editing
journal, November 2018
- Miller, Neil T.; Burnap, Robert L.
- Proceedings of the National Academy of Sciences, Vol. 115, Issue 50
Cryopreservation of Synechococcus elongatus UTEX 2973
journal, January 2019
- Racharaks, Ratanachat; Peccia, Jordan
- Journal of Applied Phycology, Vol. 31, Issue 4
Development of a longevous two-species biophotovoltaics with constrained electron flow
journal, September 2019
- Zhu, Huawei; Meng, Hengkai; Zhang, Wei
- Nature Communications, Vol. 10, Issue 1