Switchgrass (Panicum virgatum L.) Intraspecific Variation and Thermotolerance Classification Using in Vitro Seed Germination Assay
Abstract
Cardinal temperatures for plant processes have been used for thermotolerance screening of genotypes, geoclimatic adaptability determination and phenological prediction. Current simulation models for switchgrass (Panicum virgatum L.) utilize single cardinal temperatures across genotypes for both vegetative and reproductive processes although in-tra-specific variation exists among genotypes. An experiment was conducted to estimate the cardinal temperatures for seed germination of 14 diverse switchgrass genotypes and to classify genotypes for temperature tolerance. Stratified seeds of each genotype were germinated at eight constant temperatures from 10 °C to 45 °C under a constant light intensity of 35 μmol m-2s-1 for 12 hd-1. Germination was recorded at 6-h intervals in all treatments. Maximum seed germination (MSG) and germination rate (GR), estimated by fitting Sigmoidal function to germination-time series data, varied among genotypes. Quadratic and bilinear models best described the MSG and GR responses to temperature, respectively. The mean cardinal temperatures, Tmin, Topt, and Tmax, were 8.1, 26.6, and 45.1 °C for MSG and 11.1, 33.1, and 46.0 °C for GR, respectively. Cardinal temperatures for MSG and GR; however, varied significantly among genotypes. Genotypes were classified as sensitive (Cave-in-Rock, Dacotah, Expresso, Forestburg, Kanlow, Sunburst, Trailblazer, and Tusca), intermediate (Alamo, Blackwell, Carthage, Shawnee, and Shelter) and tolerantmore »
- Authors:
- Publication Date:
- Research Org.:
- Mississippi State Univ., Mississippi State, MS (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE)
- OSTI Identifier:
- 1079591
- Report Number(s):
- GO8602540
Journal ID: ISSN 2158-2742
- Grant/Contract Number:
- FG36-06GO86025
- Resource Type:
- Accepted Manuscript
- Journal Name:
- American Journal of Plant Sciences
- Additional Journal Information:
- Journal Volume: 02; Journal Issue: 02; Journal ID: ISSN 2158-2742
- Publisher:
- Scientific Research Publishing, Inc.
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; Switchgrass, Cardinal Temperature, Temperature Tolerance, Germination, Genotype Variability, Response Index, Screening, Genotype Classification
Citation Formats
Seepaul, Ramdeo, Macoon, Bisoondat, Reddy, K. Raja, and Baldwin, Brian. Switchgrass (Panicum virgatum L.) Intraspecific Variation and Thermotolerance Classification Using in Vitro Seed Germination Assay. United States: N. p., 2011.
Web. doi:10.4236/ajps.2011.22015.
Seepaul, Ramdeo, Macoon, Bisoondat, Reddy, K. Raja, & Baldwin, Brian. Switchgrass (Panicum virgatum L.) Intraspecific Variation and Thermotolerance Classification Using in Vitro Seed Germination Assay. United States. https://doi.org/10.4236/ajps.2011.22015
Seepaul, Ramdeo, Macoon, Bisoondat, Reddy, K. Raja, and Baldwin, Brian. Sat .
"Switchgrass (Panicum virgatum L.) Intraspecific Variation and Thermotolerance Classification Using in Vitro Seed Germination Assay". United States. https://doi.org/10.4236/ajps.2011.22015. https://www.osti.gov/servlets/purl/1079591.
@article{osti_1079591,
title = {Switchgrass (Panicum virgatum L.) Intraspecific Variation and Thermotolerance Classification Using in Vitro Seed Germination Assay},
author = {Seepaul, Ramdeo and Macoon, Bisoondat and Reddy, K. Raja and Baldwin, Brian},
abstractNote = {Cardinal temperatures for plant processes have been used for thermotolerance screening of genotypes, geoclimatic adaptability determination and phenological prediction. Current simulation models for switchgrass (Panicum virgatum L.) utilize single cardinal temperatures across genotypes for both vegetative and reproductive processes although in-tra-specific variation exists among genotypes. An experiment was conducted to estimate the cardinal temperatures for seed germination of 14 diverse switchgrass genotypes and to classify genotypes for temperature tolerance. Stratified seeds of each genotype were germinated at eight constant temperatures from 10 °C to 45 °C under a constant light intensity of 35 μmol m-2s-1 for 12 hd-1. Germination was recorded at 6-h intervals in all treatments. Maximum seed germination (MSG) and germination rate (GR), estimated by fitting Sigmoidal function to germination-time series data, varied among genotypes. Quadratic and bilinear models best described the MSG and GR responses to temperature, respectively. The mean cardinal temperatures, Tmin, Topt, and Tmax, were 8.1, 26.6, and 45.1 °C for MSG and 11.1, 33.1, and 46.0 °C for GR, respectively. Cardinal temperatures for MSG and GR; however, varied significantly among genotypes. Genotypes were classified as sensitive (Cave-in-Rock, Dacotah, Expresso, Forestburg, Kanlow, Sunburst, Trailblazer, and Tusca), intermediate (Alamo, Blackwell, Carthage, Shawnee, and Shelter) and tolerant (Summer) to high temperature based on cumulative temperature response index (CTRI) estimated by summing individual response indices estimated from the MSG and GR cardinal temperatures. Similarly, genotypes were also classified as sensitive (Alamo, Blackwell, Carthage, Dacotah, Shawnee, Shelter and Summer), moderately sensitive (Cave-in-rock, Forestburg, Kanlow, Sunburst, and Tusca), moderately tolerant (Trailblazer), and tolerant (Expresso) to low temperatures. The cardinal temperature estimates would be useful to improve switchgrass models for field applications. Additionally, the identified cold- and heat-tolerant genotypes can be selected for niche environments and in switchgrass breeding programs to develop new genotypes for low and high temperature environments.},
doi = {10.4236/ajps.2011.22015},
journal = {American Journal of Plant Sciences},
number = 02,
volume = 02,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 2011},
month = {Sat Jan 01 00:00:00 EST 2011}
}
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