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Title: Single nanopore transport of synthetic and biological polyelectrolytes in three-dimensional hybrid microfluidic/nanofluidic devices

Abstract

This paper presents a study of electrokinetic transport in single nanopores integrated into vertically-stacked three-dimensional hybrid microfluidic/nanofluidic structures. In these devices single nanopores, created by focused ion beam (FIB) milling in thin polymer films, provide fluidic connection between two vertically separated, perpendicular microfluidic channels. Experiments address both systems in which the nanoporous membrane is composed of the same (homojunction) or different (heterojunction) polymer as the microfluidic channels. These devices are then used to study the electrokinetic transport properties of synthetic (i.e., polystyrene sulfonate and polyallylamine) and biological (i.e.,DNA) polyelectrolytes across these nanopores. Single nanopore transport of polyelectrolytes across these nanopores using both electrical current measurements and confocal microscopy. Both optical and electrical measurements indicate that electroosmotic transport is predominant over electrophoresis in single nanopores with d > 180 nm, consistent with results obtained under similar conditions for nanocapillary array membranes.

Authors:
 [1];  [2];  [3]
+ Show Author Affiliations
  1. Univ. of Illinois at Urbana-Champaign, IL (United States). Dept. of Chemistry; Univ. of Notre Dame, South Bend, IN (United States). Dept. of Chemical and Biomolecular Engineering
  2. Univ. of Notre Dame, South Bend, IN (United States). Dept. of Chemical and Biomolecular Engineering
  3. Univ. of Notre Dame, South Bend, IN (United States). Dept. of Chemical and Biomolecular Engineering, Dept. of Chemistry and Biochemistry
Publication Date:
Research Org.:
University of Notre Dame, IN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1076433
Grant/Contract Number:  
FG02-07ER15851
Resource Type:
Accepted Manuscript
Journal Name:
Biomicrofluidics
Additional Journal Information:
Journal Volume: 3; Journal Issue: 1; Journal ID: ISSN 1932-1058
Publisher:
American Institute of Physics (AIP)
Country of Publication:
United States
Language:
English
Subject:
77 NANOSCIENCE AND NANOTECHNOLOGY; 42 ENGINEERING

Citation Formats

King, Travis L., Gatimu, Enid N., and Bohn, Paul W. Single nanopore transport of synthetic and biological polyelectrolytes in three-dimensional hybrid microfluidic/nanofluidic devices. United States: N. p., 2009. Web. doi:10.1063/1.3059546.
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King, Travis L., Gatimu, Enid N., & Bohn, Paul W. Single nanopore transport of synthetic and biological polyelectrolytes in three-dimensional hybrid microfluidic/nanofluidic devices. United States. https://doi.org/10.1063/1.3059546
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King, Travis L., Gatimu, Enid N., and Bohn, Paul W. Fri . "Single nanopore transport of synthetic and biological polyelectrolytes in three-dimensional hybrid microfluidic/nanofluidic devices". United States. https://doi.org/10.1063/1.3059546. https://www.osti.gov/servlets/purl/1076433.
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@article{osti_1076433,
title = {Single nanopore transport of synthetic and biological polyelectrolytes in three-dimensional hybrid microfluidic/nanofluidic devices},
author = {King, Travis L. and Gatimu, Enid N. and Bohn, Paul W.},
abstractNote = {This paper presents a study of electrokinetic transport in single nanopores integrated into vertically-stacked three-dimensional hybrid microfluidic/nanofluidic structures. In these devices single nanopores, created by focused ion beam (FIB) milling in thin polymer films, provide fluidic connection between two vertically separated, perpendicular microfluidic channels. Experiments address both systems in which the nanoporous membrane is composed of the same (homojunction) or different (heterojunction) polymer as the microfluidic channels. These devices are then used to study the electrokinetic transport properties of synthetic (i.e., polystyrene sulfonate and polyallylamine) and biological (i.e.,DNA) polyelectrolytes across these nanopores. Single nanopore transport of polyelectrolytes across these nanopores using both electrical current measurements and confocal microscopy. Both optical and electrical measurements indicate that electroosmotic transport is predominant over electrophoresis in single nanopores with d > 180 nm, consistent with results obtained under similar conditions for nanocapillary array membranes.},
doi = {10.1063/1.3059546},
journal = {Biomicrofluidics},
number = 1,
volume = 3,
place = {United States},
year = {Fri Jan 02 00:00:00 EST 2009},
month = {Fri Jan 02 00:00:00 EST 2009}
}
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Publisher's Version of Record
https://doi.org/10.1063/1.3059546
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