Defatted Microalgae-Mediated Enrichment of n–3 Polyunsaturated Fatty Acids in Chicken Muscle Is Not Affected by Dietary Selenium, Vitamin E, or Corn Oil
Abstract
We previously showed enrichments of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) in broiler chicks fed defatted microalgae. The aims of this study were to determine 1) if the enrichments affected meat texture and were enhanced by manipulating dietary corn oil, selenium, and vitamin E concentrations and 2) how the enrichments corroborated with hepatic gene expression involved in biosynthesis and oxidation of EPA and DHA. Day-old hatching Cornish Giant cockerels (n= 216) were divided into 6 groups (6 cages/group and 6 chicks/cage). Chicks were fed 1 of the 6 diets: a control diet containing 4% corn oil, 25 IU vitamin E/kg, and 0.2 mg Se/kg (4CO), 4CO + 10% microalgae (defatted Nannochloropsis oceanica; 4CO+ MA), 4CO+ MA – 2% corn oil (2CO+MA), 2CO+MA + 75 IU vitamin E/kg (2CO+MA+E), 2CO+MA + 0.3 mg Se/kg (2CO+MA+Se), and 2CO+MA+E + 0.3 mg Se/kg (2CO+MA+E+Se). After 6 wk, fatty acid profiles, DHA and EPA biosynthesis and oxidation, gene expression, lipid peroxidation, antioxidant status, and meat texture were measured in liver, muscles, or both. Compared with the control diet, defatted microalgae (4CO+MA) enriched (P< 0.05) DHA and EPA by ≤116 and 24 mg/100 g tissue in the liver and muscles, respectively, and downregulated (41–76%,more »
- Authors:
-
- Cornell University, Ithaca, NY (United States)
- Publication Date:
- Research Org.:
- Duke Univ., Durham, NC (United States); Cornell Univ., Ithaca, NY (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Office of Technology Development (EE-20)
- OSTI Identifier:
- 2310379
- Alternate Identifier(s):
- OSTI ID: 2229731
- Grant/Contract Number:
- EE0007091
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Nutrition
- Additional Journal Information:
- Journal Volume: 148; Journal Issue: 10; Journal ID: ISSN 0022-3166
- Publisher:
- American Society for Nutrition - Oxford University Press
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; antioxidant enzymes; broiler chicken; DHA/EPA; meat texture; microalgae
Citation Formats
Tao, Ling, Sun, Tao, Magnuson, Andrew D., Qamar, Tahir R., and Lei, Xin Gen. Defatted Microalgae-Mediated Enrichment of n–3 Polyunsaturated Fatty Acids in Chicken Muscle Is Not Affected by Dietary Selenium, Vitamin E, or Corn Oil. United States: N. p., 2018.
Web. doi:10.1093/jn/nxy164.
Tao, Ling, Sun, Tao, Magnuson, Andrew D., Qamar, Tahir R., & Lei, Xin Gen. Defatted Microalgae-Mediated Enrichment of n–3 Polyunsaturated Fatty Acids in Chicken Muscle Is Not Affected by Dietary Selenium, Vitamin E, or Corn Oil. United States. https://doi.org/10.1093/jn/nxy164
Tao, Ling, Sun, Tao, Magnuson, Andrew D., Qamar, Tahir R., and Lei, Xin Gen. Mon .
"Defatted Microalgae-Mediated Enrichment of n–3 Polyunsaturated Fatty Acids in Chicken Muscle Is Not Affected by Dietary Selenium, Vitamin E, or Corn Oil". United States. https://doi.org/10.1093/jn/nxy164. https://www.osti.gov/servlets/purl/2310379.
@article{osti_2310379,
title = {Defatted Microalgae-Mediated Enrichment of n–3 Polyunsaturated Fatty Acids in Chicken Muscle Is Not Affected by Dietary Selenium, Vitamin E, or Corn Oil},
author = {Tao, Ling and Sun, Tao and Magnuson, Andrew D. and Qamar, Tahir R. and Lei, Xin Gen},
abstractNote = {We previously showed enrichments of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) in broiler chicks fed defatted microalgae. The aims of this study were to determine 1) if the enrichments affected meat texture and were enhanced by manipulating dietary corn oil, selenium, and vitamin E concentrations and 2) how the enrichments corroborated with hepatic gene expression involved in biosynthesis and oxidation of EPA and DHA. Day-old hatching Cornish Giant cockerels (n= 216) were divided into 6 groups (6 cages/group and 6 chicks/cage). Chicks were fed 1 of the 6 diets: a control diet containing 4% corn oil, 25 IU vitamin E/kg, and 0.2 mg Se/kg (4CO), 4CO + 10% microalgae (defatted Nannochloropsis oceanica; 4CO+ MA), 4CO+ MA – 2% corn oil (2CO+MA), 2CO+MA + 75 IU vitamin E/kg (2CO+MA+E), 2CO+MA + 0.3 mg Se/kg (2CO+MA+Se), and 2CO+MA+E + 0.3 mg Se/kg (2CO+MA+E+Se). After 6 wk, fatty acid profiles, DHA and EPA biosynthesis and oxidation, gene expression, lipid peroxidation, antioxidant status, and meat texture were measured in liver, muscles, or both. Compared with the control diet, defatted microalgae (4CO+MA) enriched (P< 0.05) DHA and EPA by ≤116 and 24 mg/100 g tissue in the liver and muscles, respectively, and downregulated (41–76%, P< 0.01) hepatic mRNA abundance of 4 cytochrome P450 (CYP) enzymes (CYP2C23b, CYP2D6, CYP3A5, CYP4V2). Supplemental microalgae decreased (50–82%, P< 0.05) lipid peroxidation and improved (16–28%, P< 0.05) antioxidant status in the liver, muscles, or both. Furthermore, the microalgae-mediated enrichments in the muscles were not elevated by altering dietary corn oil, vitamin E, or selenium and did not affect meat texture. The microalgae-mediated enrichments of DHA and EPA in the chicken muscles were associated with decreased hepatic gene expression of their oxidation, but were not further enhanced by altering dietary corn oil, vitamin E, or selenium.},
doi = {10.1093/jn/nxy164},
journal = {Journal of Nutrition},
number = 10,
volume = 148,
place = {United States},
year = {Mon Sep 10 00:00:00 EDT 2018},
month = {Mon Sep 10 00:00:00 EDT 2018}
}
Web of Science
Works referenced in this record:
Fatty Acid Desaturases, Polyunsaturated Fatty Acid Regulation, and Biotechnological Advances
journal, January 2016
- Lee, Je; Lee, Hyungjae; Kang, SeokBeom
- Nutrients, Vol. 8, Issue 1
Omega-3 Fatty Acids EPA and DHA: Health Benefits Throughout Life
journal, January 2012
- Swanson, Danielle; Block, Robert; Mousa, Shaker A.
- Advances in Nutrition, Vol. 3, Issue 1
Comparative energy life-cycle analyses of microalgal biomass production in open ponds and photobioreactors
journal, February 2010
- Jorquera, Orlando; Kiperstok, Asher; Sales, Emerson A.
- Bioresource Technology, Vol. 101, Issue 4
Oxidation of Polyunsaturated Fatty Acids and its Impact on Food Quality and Human Health
journal, December 2015
- Tao, Ling
- Advances in Food Technology and Nutritional Sciences - Open Journal, Vol. 1, Issue 6
Creating ω-3 Fatty-Acid-Enriched Chicken Using Defatted Green Microalgal Biomass
journal, October 2015
- Gatrell, Stephanie K.; Kim, JongGun; Derksen, Theodore J.
- Journal of Agricultural and Food Chemistry, Vol. 63, Issue 42
Physiological Effects of Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA)—A Review
journal, September 2006
- Narayan, Bhaskar; Miyashita, Kazuo; Hosakawa, Masashi
- Food Reviews International, Vol. 22, Issue 3
A validated liquid chromatography method for the simultaneous determination of vitamins A and E in human plasma
journal, August 2007
- Siluk, Danuta; Oliveira, Regina V.; Esther-Rodriguez-Rosas, Maria
- Journal of Pharmaceutical and Biomedical Analysis, Vol. 44, Issue 4
Simultaneous flue gas bioremediation and reduction of microalgal biomass production costs
journal, December 2008
- Douskova, I.; Doucha, J.; Livansky, K.
- Applied Microbiology and Biotechnology, Vol. 82, Issue 1
Metabolism of eicosapentaenoic and docosahexaenoic acids by recombinant human cytochromes P450
journal, March 2008
- Fer, Maude; Dréano, Yvonne; Lucas, Danièle
- Archives of Biochemistry and Biophysics, Vol. 471, Issue 2
Alleviative effects of litchi ( Litchi chinensis Sonn .) flower on lipid peroxidation and protein degradation in emulsified pork meatballs
journal, September 2015
- Ding, Yi; Wang, Sheng-Yao; Yang, Deng-Jye
- Journal of Food and Drug Analysis, Vol. 23, Issue 3
Dietary oxidized n -3 PUFA induce oxidative stress and inflammation: role of intestinal absorption of 4-HHE and reactivity in intestinal cells
journal, August 2012
- Awada, Manar; Soulage, Christophe O.; Meynier, Anne
- Journal of Lipid Research, Vol. 53, Issue 10
Glutathione peroxidase activity in rat lens and other tissues in relation to dietary selenium intake
journal, June 1974
- Lawrence, Richard A.; Sunde, Roger A.; Schwartz, Gary L.
- Experimental Eye Research, Vol. 18, Issue 6
Effect of vitamin E and selenium supplementation of cockerel diets on glutathione peroxidase activity and lipid peroxidation susceptibility in sperm, testes, and liver
journal, June 1998
- Surai, Peter; Kostjuk, Inna; Wishart, Graham
- Biological Trace Element Research, Vol. 64, Issue 1-3
Distribution, interconversion, and dose response of n−3 fatty acids in humans
journal, June 2006
- Arterburn, Linda M.; Hall, Eileen Bailey; Oken, Harry
- The American Journal of Clinical Nutrition, Vol. 83, Issue 6
Potential and Limitation of a New Defatted Diatom Microalgal Biomass in Replacing Soybean Meal and Corn in Diets for Broiler Chickens
journal, July 2013
- Austic, Richard E.; Mustafa, Ahsan; Jung, Byoungyun
- Journal of Agricultural and Food Chemistry, Vol. 61, Issue 30
Dual potential of microalgae as a sustainable biofuel feedstock and animal feed
journal, January 2013
- Lum, Krystal K.; Kim, Jonggun; Lei, Xin
- Journal of Animal Science and Biotechnology, Vol. 4, Issue 1
Membrane lipid unsaturation as physiological adaptation to animal longevity
journal, January 2013
- Naudí, Alba; Jové, Mariona; Ayala, Victòria
- Frontiers in Physiology, Vol. 4
Inhibition of purified rat liver glutathione S-transferase isozymes by diuretic drugs
journal, June 1985
- Ahokas, Jorma T.; Nicholls, Felicity A.; Ravenscroft, Peter J.
- Biochemical Pharmacology, Vol. 34, Issue 12
Phospholipase A 2 Enzymes: Physical Structure, Biological Function, Disease Implication, Chemical Inhibition, and Therapeutic Intervention
journal, October 2011
- Dennis, Edward A.; Cao, Jian; Hsu, Yuan-Hao
- Chemical Reviews, Vol. 111, Issue 10
Antioxidant Activity of Hawaiian Marine Algae
journal, February 2012
- Kelman, Dovi; Posner, Ellen Kromkowski; McDermid, Karla J.
- Marine Drugs, Vol. 10, Issue 12
Potential of combining flaxseed oil and microalgal biomass in producing eggs-enriched with n − 3 fatty acids for meeting human needs
journal, July 2016
- Kim, Jonggun; Magnuson, Andrew; Tao, Ling
- Algal Research, Vol. 17
Antioxidant Activity of Algal Extracts on Lipid Peroxidation
journal, January 2003
- ., Ahmed M. Aboul-Enein; ., Farouk K. El Baz; ., Gamal S. El-Baroty
- Journal of Medical Sciences(Faisalabad), Vol. 3, Issue 1
Long-chain conversion of [ 13 C]linoleic acid and α-linolenic acid in response to marked changes in their dietary intake in men
journal, December 2004
- Hussein, Nahed; Ah-Sing, Eric; Wilkinson, Paul
- Journal of Lipid Research, Vol. 46, Issue 2
Polyunsaturated fatty acids in the food chain in the United States
journal, January 2000
- Kris-Etherton, Pm; Taylor, Denise Shaffer; Yu-Poth, Shaomei
- The American Journal of Clinical Nutrition, Vol. 71, Issue 1
Functional Characterization of the Chicken Fatty Acid Elongases
journal, November 2012
- Gregory, Melissa K.; Geier, Mark S.; Gibson, Robert A.
- The Journal of Nutrition, Vol. 143, Issue 1
A Simple Method for the Isolation and Purification of Total Lipides from Animal Tissues
journal, May 1957
- Folch, Jordi; Lees, M.; Stanley, G. H. Sloane
- Journal of Biological Chemistry, Vol. 226, Issue 1
Cyclooxygenases: Structural, Cellular, and Molecular Biology
journal, June 2000
- Smith, William L.; DeWitt, David L.; Garavito, R. Michael
- Annual Review of Biochemistry, Vol. 69, Issue 1
Vitamin E and selenium in broiler breeder diets: Effect on live performance, hatching process, and chick quality
journal, May 2015
- Urso, U. R. A.; Dahlke, F.; Maiorka, A.
- Poultry Science, Vol. 94, Issue 5
Microalgae biomass as an alternative ingredient in cookies: Sensory, physical and chemical properties, antioxidant activity and in vitro digestibility
journal, September 2017
- Batista, Ana Paula; Niccolai, Alberto; Fradinho, Patrícia
- Algal Research, Vol. 26
Effect of vitamin E supplementation on arsenic induced alteration in blood biochemical profile, oxidant/antioxidant status, serum cortisol level and retention of arsenic and selenium in goats
journal, January 2015
- Mohanta, Ranjan Kumar; Garg, Anil Kumar; Dass, Ram Sharan
- Journal of Trace Elements in Medicine and Biology, Vol. 29
Antioxidant effect of the marine algae Chlorella vulgaris against naphthalene-induced oxidative stress in the albino rats
journal, April 2007
- Vijayavel, K.; Anbuselvam, C.; Balasubramanian, M. P.
- Molecular and Cellular Biochemistry, Vol. 303, Issue 1-2
Effects of dietary ω -3 fatty acid-enriched microalgae supplementation on growth performance, blood profiles, meat quality, and fatty acid composition of meat in broilers
journal, December 2013
- Yan, L.; Kim, I. H.
- Journal of Applied Animal Research, Vol. 41, Issue 4
Lipoxygenases: Potential starting biocatalysts for the synthesis of signaling compounds
journal, November 2012
- Joo, Young-Chul; Oh, Deok-Kun
- Biotechnology Advances, Vol. 30, Issue 6