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Structure-Driven Liquid Microjunction Surface-Sampling Probe Mass Spectrometry
Abstract
The rhizosphere is the narrow region of soil surrounding the roots of plants that is influenced by root exudates, root secretions, and associated microbial communities. This region is crucial to plant growth and development and plays a critical role in nutrient uptake, disease resistance, and soil transformation. Understanding the function of exogenous compounds in the rhizosphere starts with determining the spatiotemporal distribution of these molecular components. Using liquid microjunction surface-sampling probe mass spectrometry (LMJ-SSP-MS) and microfluidic devices with attached microporous membranes enables in situ, nondisruptive, and nondestructive spatiotemporal measurement of exogenous compounds from plant roots. However, long imaging times (>2 h) can negatively affect plant heath and limit temporal studies. Here, we present a novel strategy to optimize the number and location of sampling sites on these microporous membrane-covered microfluidic devices. In conclusion, this novel, “structure-driven” sampling workflow takes into consideration the channel structure of the microfluidic device to maximize sampling from the channels and minimize acquisition time (~4× less time in some cases while providing similar chemical image accuracy), thus reducing stress on plants during in situ LMJ-SSP-MS analysis.
- Authors:
-
[1];
[2];
[1]
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences (CNMS)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 2076197
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Analytical Chemistry
- Additional Journal Information:
- Journal Volume: 95; Journal Issue: 39; Journal ID: ISSN 0003-2700
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; computer simulations; fluorescence; mass spectrometry; microfluidic devices; plants
Citation Formats
Kertesz, Vilmos, Khalid, Muneeba, Retterer, Scott T., and Cahill, John F. Structure-Driven Liquid Microjunction Surface-Sampling Probe Mass Spectrometry. United States: N. p., 2023.
Web. doi:10.1021/acs.analchem.3c02370.
Kertesz, Vilmos, Khalid, Muneeba, Retterer, Scott T., & Cahill, John F. Structure-Driven Liquid Microjunction Surface-Sampling Probe Mass Spectrometry. United States. https://doi.org/10.1021/acs.analchem.3c02370
Kertesz, Vilmos, Khalid, Muneeba, Retterer, Scott T., and Cahill, John F. Fri .
"Structure-Driven Liquid Microjunction Surface-Sampling Probe Mass Spectrometry". United States. https://doi.org/10.1021/acs.analchem.3c02370.
@article{osti_2076197,
title = {Structure-Driven Liquid Microjunction Surface-Sampling Probe Mass Spectrometry},
author = {Kertesz, Vilmos and Khalid, Muneeba and Retterer, Scott T. and Cahill, John F.},
abstractNote = {The rhizosphere is the narrow region of soil surrounding the roots of plants that is influenced by root exudates, root secretions, and associated microbial communities. This region is crucial to plant growth and development and plays a critical role in nutrient uptake, disease resistance, and soil transformation. Understanding the function of exogenous compounds in the rhizosphere starts with determining the spatiotemporal distribution of these molecular components. Using liquid microjunction surface-sampling probe mass spectrometry (LMJ-SSP-MS) and microfluidic devices with attached microporous membranes enables in situ, nondisruptive, and nondestructive spatiotemporal measurement of exogenous compounds from plant roots. However, long imaging times (>2 h) can negatively affect plant heath and limit temporal studies. Here, we present a novel strategy to optimize the number and location of sampling sites on these microporous membrane-covered microfluidic devices. In conclusion, this novel, “structure-driven” sampling workflow takes into consideration the channel structure of the microfluidic device to maximize sampling from the channels and minimize acquisition time (~4× less time in some cases while providing similar chemical image accuracy), thus reducing stress on plants during in situ LMJ-SSP-MS analysis.},
doi = {10.1021/acs.analchem.3c02370},
journal = {Analytical Chemistry},
number = 39,
volume = 95,
place = {United States},
year = {Fri Sep 22 00:00:00 EDT 2023},
month = {Fri Sep 22 00:00:00 EDT 2023}
}
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