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Title: Distinct Microbial Assemblage Structure and Archaeal Diversity in Sediments of Arctic Thermokarst Lakes Differing in Methane Sources

Abstract

Developing a microbial ecological understanding of Arctic thermokarst lake sediments in a geochemical context is an essential first step toward comprehending the contributions of these systems to greenhouse gas emissions, and understanding how they may shift as a result of long term changes in climate. In light of this, we set out to study microbial diversity and structure in sediments from four shallow thermokarst lakes in the Arctic Coastal Plain of Alaska. Sediments from one of these lakes (Sukok) emit methane (CH4) of thermogenic origin, as expected for an area with natural gas reserves. However, sediments from a lake 10 km to the North West (Siqlukaq) produce CH4 of biogenic origin. Sukok and Siqlukaq were chosen among the four lakes surveyed to test the hypothesis that active CH4-producing organisms (methanogens) would reflect the distribution of CH4 gas levels in the sediments. We first examined the structure of the little known microbial community inhabiting the thaw bulb of arctic thermokarst lakes near Barrow, AK. Molecular approaches (PCR-DGGE and iTag sequencing) targeting the SSU rRNA gene and rRNA molecule were used to profile diversity, assemblage structure, and identify potentially active members of the microbial assemblages. Overall, the potentially active (rRNA dominant) fractionmore » included taxa that have also been detected in other permafrost environments (e.g., Bacteroidetes, Actinobacteria, Nitrospirae, Chloroflexi, and others). In addition, Siqlukaq sediments were unique compared to the other sites, in that they harbored CH4-cycling organisms (i.e., methanogenic Archaea and methanotrophic Bacteria), as well as bacteria potentially involved in N cycling (e.g., Nitrospirae) whereas Sukok sediments were dominated by taxa typically involved in photosynthesis and biogeochemical sulfur (S) transformations. This study revealed a high degree of archaeal phylogenetic diversity in addition to CH4-producing archaea, which spanned nearly the phylogenetic extent of currently recognized Archaea phyla (e.g., Euryarchaeota, Bathyarchaeota, Thaumarchaeota, Woesearchaeota, Pacearchaeota, and others). Together these results shed light on expansive bacterial and archaeal diversity in Arctic thermokarst lakes and suggest important differences in biogeochemical potential in contrasting Arctic thermokarst lake sediment ecosystems.« less

Authors:
 [1];  [2];  [3];  [4];  [1]
+ Show Author Affiliations
  1. Desert Research Institute (DRI), Reno, NV (United States)
  2. Univ. of Vienna (Austria)
  3. California Institute of Technology (CalTech), Pasadena, CA (United States)
  4. Montana State Univ., Bozeman, MT (United States)
Publication Date:
Research Org.:
USDOE Joint Genome Institute (JGI), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1904080
Grant/Contract Number:  
AC02-05CH11231; JGI-634
Resource Type:
Accepted Manuscript
Journal Name:
Frontiers in Microbiology
Additional Journal Information:
Journal Volume: 9; Journal ID: ISSN 1664-302X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; methane; thermokarst; lake, sediments; Arctic; Alaska; archaea; diversity

Citation Formats

Matheus Carnevali, Paula B., Herbold, Craig W., Hand, Kevin P., Priscu, John C., and Murray, Alison E. Distinct Microbial Assemblage Structure and Archaeal Diversity in Sediments of Arctic Thermokarst Lakes Differing in Methane Sources. United States: N. p., 2018. Web. doi:10.3389/fmicb.2018.01192.
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Matheus Carnevali, Paula B., Herbold, Craig W., Hand, Kevin P., Priscu, John C., & Murray, Alison E. Distinct Microbial Assemblage Structure and Archaeal Diversity in Sediments of Arctic Thermokarst Lakes Differing in Methane Sources. United States. https://doi.org/10.3389/fmicb.2018.01192
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Matheus Carnevali, Paula B., Herbold, Craig W., Hand, Kevin P., Priscu, John C., and Murray, Alison E. Thu . "Distinct Microbial Assemblage Structure and Archaeal Diversity in Sediments of Arctic Thermokarst Lakes Differing in Methane Sources". United States. https://doi.org/10.3389/fmicb.2018.01192. https://www.osti.gov/servlets/purl/1904080.
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@article{osti_1904080,
title = {Distinct Microbial Assemblage Structure and Archaeal Diversity in Sediments of Arctic Thermokarst Lakes Differing in Methane Sources},
author = {Matheus Carnevali, Paula B. and Herbold, Craig W. and Hand, Kevin P. and Priscu, John C. and Murray, Alison E.},
abstractNote = {Developing a microbial ecological understanding of Arctic thermokarst lake sediments in a geochemical context is an essential first step toward comprehending the contributions of these systems to greenhouse gas emissions, and understanding how they may shift as a result of long term changes in climate. In light of this, we set out to study microbial diversity and structure in sediments from four shallow thermokarst lakes in the Arctic Coastal Plain of Alaska. Sediments from one of these lakes (Sukok) emit methane (CH4) of thermogenic origin, as expected for an area with natural gas reserves. However, sediments from a lake 10 km to the North West (Siqlukaq) produce CH4 of biogenic origin. Sukok and Siqlukaq were chosen among the four lakes surveyed to test the hypothesis that active CH4-producing organisms (methanogens) would reflect the distribution of CH4 gas levels in the sediments. We first examined the structure of the little known microbial community inhabiting the thaw bulb of arctic thermokarst lakes near Barrow, AK. Molecular approaches (PCR-DGGE and iTag sequencing) targeting the SSU rRNA gene and rRNA molecule were used to profile diversity, assemblage structure, and identify potentially active members of the microbial assemblages. Overall, the potentially active (rRNA dominant) fraction included taxa that have also been detected in other permafrost environments (e.g., Bacteroidetes, Actinobacteria, Nitrospirae, Chloroflexi, and others). In addition, Siqlukaq sediments were unique compared to the other sites, in that they harbored CH4-cycling organisms (i.e., methanogenic Archaea and methanotrophic Bacteria), as well as bacteria potentially involved in N cycling (e.g., Nitrospirae) whereas Sukok sediments were dominated by taxa typically involved in photosynthesis and biogeochemical sulfur (S) transformations. This study revealed a high degree of archaeal phylogenetic diversity in addition to CH4-producing archaea, which spanned nearly the phylogenetic extent of currently recognized Archaea phyla (e.g., Euryarchaeota, Bathyarchaeota, Thaumarchaeota, Woesearchaeota, Pacearchaeota, and others). Together these results shed light on expansive bacterial and archaeal diversity in Arctic thermokarst lakes and suggest important differences in biogeochemical potential in contrasting Arctic thermokarst lake sediment ecosystems.},
doi = {10.3389/fmicb.2018.01192},
journal = {Frontiers in Microbiology},
number = ,
volume = 9,
place = {United States},
year = {Thu Jun 07 00:00:00 EDT 2018},
month = {Thu Jun 07 00:00:00 EDT 2018}
}
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https://doi.org/10.3389/fmicb.2018.01192
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Works referenced in this record:

Methane sources in arctic thermokarst lake sediments on the North Slope of Alaska
journal, January 2015

  • Matheus Carnevali, P. B.; Rohrssen, M.; Williams, M. R.
  • Geobiology, Vol. 13, Issue 2
  • DOI: 10.1111/gbi.12124

Organic carbon transformations in high-Arctic peat soils: key functions and microorganisms
journal, September 2012

  • Tveit, Alexander; Schwacke, Rainer; Svenning, Mette M.
  • The ISME Journal, Vol. 7, Issue 2
  • DOI: 10.1038/ismej.2012.99

Metagenomic Insights into Anaerobic Metabolism along an Arctic Peat Soil Profile
journal, May 2013

Methane metabolism in the archaeal phylum Bathyarchaeota revealed by genome-centric metagenomics
journal, October 2015

The recent warming of permafrost in Alaska
journal, December 2005

Cohn’s Crenothrix is a filamentous methane oxidizer with an unusual methane monooxygenase
journal, February 2006

  • Stoecker, Kilian; Bendinger, Bernd; Schöning, Björn
  • Proceedings of the National Academy of Sciences, Vol. 103, Issue 7
  • DOI: 10.1073/pnas.0506361103

Iron-Oxidizing Bacteria: An Environmental and Genomic Perspective
journal, October 2010

Microbial community diversity and heterotrophic production in a coastal Arctic ecosystem: A stamukhi lake and its source waters
journal, March 2008

  • Galand, Pierre E.; Lovejoy, Connie; Pouliot, JÉrÉmie
  • Limnology and Oceanography, Vol. 53, Issue 2
  • DOI: 10.4319/lo.2008.53.2.0813

Rare but active taxa contribute to community dynamics of benthic biofilms in glacier-fed streams: Benthic biofilms in glacier-fed streams
journal, February 2014

  • Wilhelm, Linda; Besemer, Katharina; Fasching, Christina
  • Environmental Microbiology, Vol. 16, Issue 8
  • DOI: 10.1111/1462-2920.12392

Environmental selection of planktonic methanogens in permafrost thaw ponds
journal, August 2016

  • Crevecoeur, Sophie; Vincent, Warwick F.; Lovejoy, Connie
  • Scientific Reports, Vol. 6, Issue 1
  • DOI: 10.1038/srep31312

Methylobacter tundripaludum sp. nov., a methane-oxidizing bacterium from Arctic wetland soil on the Svalbard islands, Norway (78  N)
journal, January 2006

  • Wartiainen, I.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 56, Issue 1
  • DOI: 10.1099/ijs.0.63728-0

Microbial diversity of active layer and permafrost in an acidic wetland from the Canadian High Arctic
journal, April 2011

  • Wilhelm, Roland C.; Niederberger, Thomas D.; Greer, Charles
  • Canadian Journal of Microbiology, Vol. 57, Issue 4
  • DOI: 10.1139/w11-004

Methanomassiliicoccus luminyensis gen. nov., sp. nov., a methanogenic archaeon isolated from human faeces
journal, August 2012

  • Dridi, B.; Fardeau, M. -L.; Ollivier, B.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 62, Issue Pt 8
  • DOI: 10.1099/ijs.0.033712-0

Metagenomics Reveals Pervasive Bacterial Populations and Reduced Community Diversity across the Alaska Tundra Ecosystem
journal, April 2016

  • Johnston, Eric R.; Rodriguez-R, Luis M.; Luo, Chengwei
  • Frontiers in Microbiology, Vol. 7
  • DOI: 10.3389/fmicb.2016.00579

Development of a Dual-Index Sequencing Strategy and Curation Pipeline for Analyzing Amplicon Sequence Data on the MiSeq Illumina Sequencing Platform
journal, June 2013

  • Kozich, James J.; Westcott, Sarah L.; Baxter, Nielson T.
  • Applied and Environmental Microbiology, Vol. 79, Issue 17
  • DOI: 10.1128/AEM.01043-13

Simulated rRNA/DNA Ratios Show Potential To Misclassify Active Populations as Dormant
journal, March 2017

  • Steven, Blaire; Hesse, Cedar; Soghigian, John
  • Applied and Environmental Microbiology, Vol. 83, Issue 11
  • DOI: 10.1128/AEM.00696-17

Crenothrix are major methane consumers in stratified lakes
journal, June 2017

  • Oswald, Kirsten; Graf, Jon S.; Littmann, Sten
  • The ISME Journal, Vol. 11, Issue 9
  • DOI: 10.1038/ismej.2017.77

Evaluating rRNA as an indicator of microbial activity in environmental communities: limitations and uses
journal, July 2013

  • Blazewicz, Steven J.; Barnard, Romain L.; Daly, Rebecca A.
  • The ISME Journal, Vol. 7, Issue 11
  • DOI: 10.1038/ismej.2013.102

Trimmomatic: a flexible trimmer for Illumina sequence data
journal, April 2014

Methane cycling dynamics in sediments of Alaskan Arctic Foothill lakes
journal, January 2014

The functional potential of high Arctic permafrost revealed by metagenomic sequencing, qPCR and microarray analyses
journal, April 2010

  • Yergeau, Etienne; Hogues, Hervé; Whyte, Lyle G.
  • The ISME Journal, Vol. 4, Issue 9
  • DOI: 10.1038/ismej.2010.41

Archaea catalyze iron-dependent anaerobic oxidation of methane
journal, October 2016

  • Ettwig, Katharina F.; Zhu, Baoli; Speth, Daan
  • Proceedings of the National Academy of Sciences, Vol. 113, Issue 45
  • DOI: 10.1073/pnas.1609534113

A Nitrospira metagenome illuminates the physiology and evolution of globally important nitrite-oxidizing bacteria
journal, July 2010

  • Lucker, S.; Wagner, M.; Maixner, F.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 30
  • DOI: 10.1073/pnas.1003860107

The under-recognized dominance of Verrucomicrobia in soil bacterial communities
journal, July 2011

Seasonality of rDNA- and rRNA-derived archaeal communities and methanogenic potential in a boreal mire
journal, July 2008

  • Juottonen, Heli; Tuittila, Eeva-Stiina; Juutinen, Sari
  • The ISME Journal, Vol. 2, Issue 11
  • DOI: 10.1038/ismej.2008.66

Effect of temperature on methane dynamics and evaluation of methane oxidation kinetics in shallow Arctic Alaskan lakes
journal, August 2013

Molecular and conventional analyses of microbial diversity in mesophilic and thermophilic upflow anaerobic sludge blanket granular sludges
journal, May 2002

  • Sekiguchi, Y.; Kamagata, Y.; Ohashi, A.
  • Water Science and Technology, Vol. 45, Issue 10
  • DOI: 10.2166/wst.2002.0279

Evidence of global-scale aeolian dispersal and endemism in isolated geothermal microbial communities of Antarctica
journal, May 2014

  • Herbold, Craig W.; Lee, Charles K.; McDonald, Ian R.
  • Nature Communications, Vol. 5, Issue 1
  • DOI: 10.1038/ncomms4875

Microbes in thawing permafrost: the unknown variable in the climate change equation
journal, November 2011

  • Graham, David E.; Wallenstein, Matthew D.; Vishnivetskaya, Tatiana A.
  • The ISME Journal, Vol. 6, Issue 4
  • DOI: 10.1038/ismej.2011.163

Genomic Expansion of Domain Archaea Highlights Roles for Organisms from New Phyla in Anaerobic Carbon Cycling
journal, March 2015

  • Castelle, Cindy J.; Wrighton, Kelly C.; Thomas, Brian C.
  • Current Biology, Vol. 25, Issue 6
  • DOI: 10.1016/j.cub.2015.01.014

Lokiarchaeota Marks the Transition between the Archaeal and Eukaryotic Selenocysteine Encoding Systems
journal, July 2016

  • Mariotti, Marco; Lobanov, Alexei V.; Manta, Bruno
  • Molecular Biology and Evolution, Vol. 33, Issue 9
  • DOI: 10.1093/molbev/msw122

Microbial Functional Potential and Community Composition in Permafrost-Affected Soils of the NW Canadian Arctic
journal, January 2014

Diversity of active aerobic methanotrophs along depth profiles of arctic and subarctic lake water column and sediments
journal, May 2012

  • He, Ruo; Wooller, Matthew J.; Pohlman, John W.
  • The ISME Journal, Vol. 6, Issue 10
  • DOI: 10.1038/ismej.2012.34

RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies
journal, January 2014

Effects of temperature on the diversity and community structure of known methanogenic groups and other archaea in high Arctic peat
journal, November 2007

  • Høj, Lone; Olsen, Rolf A.; Torsvik, Vigdis L.
  • The ISME Journal, Vol. 2, Issue 1
  • DOI: 10.1038/ismej.2007.84

Bacterial community composition and diversity of five different permafrost-affected soils of Northeast Greenland
journal, June 2014

  • Ganzert, Lars; Bajerski, Felizitas; Wagner, Dirk
  • FEMS Microbiology Ecology, Vol. 89, Issue 2
  • DOI: 10.1111/1574-6941.12352

Metagenomic analysis of organic matter degradation in methane-rich Arctic Ocean sediments
journal, March 2014

  • Kirchman, David L.; Hanson, Thomas E.; Cottrell, Matthew T.
  • Limnology and Oceanography, Vol. 59, Issue 2
  • DOI: 10.4319/lo.2014.59.2.0548

SINA: Accurate high-throughput multiple sequence alignment of ribosomal RNA genes
journal, May 2012

Archaea of the Miscellaneous Crenarchaeotal Group are abundant, diverse and widespread in marine sediments
journal, May 2012

  • Kubo, Kyoko; Lloyd, Karen G.; F. Biddle, Jennifer
  • The ISME Journal, Vol. 6, Issue 10
  • DOI: 10.1038/ismej.2012.37

Microbial Community Structure in Lake and Wetland Sediments from a High Arctic Polar Desert Revealed by Targeted Transcriptomics
journal, March 2014

Methane turnover and environmental change from Holocene lipid biomarker records in a thermokarst lake in Arctic Alaska
journal, July 2016

Complete nitrification by Nitrospira bacteria
journal, November 2015

  • Daims, Holger; Lebedeva, Elena V.; Pjevac, Petra
  • Nature, Vol. 528, Issue 7583
  • DOI: 10.1038/nature16461

Multi-omics of permafrost, active layer and thermokarst bog soil microbiomes
journal, March 2015

  • Hultman, Jenni; Waldrop, Mark P.; Mackelprang, Rachel
  • Nature, Vol. 521, Issue 7551
  • DOI: 10.1038/nature14238

Small Thaw Ponds: An Unaccounted Source of Methane in the Canadian High Arctic
journal, November 2013

Expanded metabolic versatility of ubiquitous nitrite-oxidizing bacteria from the genus Nitrospira
journal, August 2015

  • Koch, Hanna; Lücker, Sebastian; Albertsen, Mads
  • Proceedings of the National Academy of Sciences, Vol. 112, Issue 36
  • DOI: 10.1073/pnas.1506533112

Anaerobic oxidation of methane coupled to nitrate reduction in a novel archaeal lineage
journal, July 2013

  • Haroon, Mohamed F.; Hu, Shihu; Shi, Ying
  • Nature, Vol. 500, Issue 7464
  • DOI: 10.1038/nature12375

Genome-Based Microbial Taxonomy Coming of Age
journal, March 2016

  • Hugenholtz, Philip; Skarshewski, Adam; Parks, Donovan H.
  • Cold Spring Harbor Perspectives in Biology, Vol. 8, Issue 6
  • DOI: 10.1101/cshperspect.a018085

Long-term persistence of bacterial DNA
journal, January 2004

Novel Euryarchaeotal Lineages Detected on Rice Roots and in the Anoxic Bulk Soil of Flooded Rice Microcosms
journal, December 1998

Sensitivity of the carbon cycle in the Arctic to climate change
journal, November 2009

  • McGuire, A. David; Anderson, Leif G.; Christensen, Torben R.
  • Ecological Monographs, Vol. 79, Issue 4
  • DOI: 10.1890/08-2025.1

A new view of the tree of life
journal, April 2016

QIIME allows analysis of high-throughput community sequencing data
journal, April 2010

  • Caporaso, J. Gregory; Kuczynski, Justin; Stombaugh, Jesse
  • Nature Methods, Vol. 7, Issue 5
  • DOI: 10.1038/nmeth.f.303

The Genome Sequence of the Obligately Chemolithoautotrophic, Facultatively Anaerobic Bacterium Thiobacillus denitrificans
journal, February 2006

Methanogenic Pathway and Archaeal Community Structure in the Sediment of Eutrophic Lake Dagow: Effect of Temperature
journal, June 2004

Genome Sequence of the Arctic Methanotroph Methylobacter tundripaludum SV96
journal, July 2011

  • Svenning, Mette M.; Hestnes, Anne Grethe; Wartiainen, Ingvild
  • Journal of Bacteriology, Vol. 193, Issue 22
  • DOI: 10.1128/JB.05380-11

Archaeal communities of Arctic methane-containing permafrost
journal, June 2016

  • Shcherbakova, Victoria; Yoshimura, Yoshitaka; Ryzhmanova, Yana
  • FEMS Microbiology Ecology, Vol. 92, Issue 10
  • DOI: 10.1093/femsec/fiw135

Microbial community composition and in silico predicted metabolic potential reflect biogeochemical gradients between distinct peatland types
journal, September 2014

Activity of abundant and rare bacteria in a coastal ocean
journal, July 2011

  • Campbell, B. J.; Yu, L.; Heidelberg, J. F.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 31
  • DOI: 10.1073/pnas.1101405108

Archaea in coastal marine environments.
journal, June 1992

Taxonomy, Physiology, and Natural Products of Actinobacteria
journal, November 2015

  • Barka, Essaid Ait; Vatsa, Parul; Sanchez, Lisa
  • Microbiology and Molecular Biology Reviews, Vol. 80, Issue 1
  • DOI: 10.1128/MMBR.00019-15

Complex archaea that bridge the gap between prokaryotes and eukaryotes
journal, May 2015

  • Spang, Anja; Saw, Jimmy H.; Jørgensen, Steffen L.
  • Nature, Vol. 521, Issue 7551
  • DOI: 10.1038/nature14447

Vertical distribution and phylogenetic characterization of marine planktonic Archaea in the Santa Barbara Channel.
journal, January 1997

Biological responses to permafrost thaw slumping in Canadian Arctic lakes
journal, December 2012

  • Thienpont, Joshua R.; RÜHland, Kathleen M.; Pisaric, Michael F. J.
  • Freshwater Biology, Vol. 58, Issue 2
  • DOI: 10.1111/fwb.12061

Insights into the phylogeny and coding potential of microbial dark matter
journal, July 2013

  • Rinke, Christian; Schwientek, Patrick; Sczyrba, Alexander
  • Nature, Vol. 499, Issue 7459
  • DOI: 10.1038/nature12352

Microbial diversity in arctic freshwaters is structured by inoculation of microbes from soils
journal, March 2012

  • Crump, Byron C.; Amaral-Zettler, Linda A.; Kling, George W.
  • The ISME Journal, Vol. 6, Issue 9
  • DOI: 10.1038/ismej.2012.9

Genetic and functional properties of uncultivated MCG archaea assessed by metagenome and gene expression analyses
journal, October 2013

Thermokarst lake methanogenesis along a complete talik profile
journal, January 2015

  • Heslop, J. K.; Walter Anthony, K. M.; Sepulveda-Jauregui, A.
  • Biogeosciences, Vol. 12, Issue 14
  • DOI: 10.5194/bg-12-4317-2015

Phylogenomic Data Support a Seventh Order of Methylotrophic Methanogens and Provide Insights into the Evolution of Methanogenesis
journal, August 2013

  • Borrel, Guillaume; O’Toole, Paul W.; Harris, Hugh M. B.
  • Genome Biology and Evolution, Vol. 5, Issue 10
  • DOI: 10.1093/gbe/evt128

Remarkably diverse and contrasting archaeal communities in a large arctic river and the coastal Arctic Ocean
journal, September 2006

  • Galand, Pe; Lovejoy, C.; Vincent, Wf
  • Aquatic Microbial Ecology, Vol. 44
  • DOI: 10.3354/ame044115

Distribution of sediment bacterial and archaeal communities in plateau freshwater lakes
journal, November 2014

  • Zhang, Jingxu; Yang, Yuyin; Zhao, Lei
  • Applied Microbiology and Biotechnology, Vol. 99, Issue 7
  • DOI: 10.1007/s00253-014-6262-x

The iron-oxidizing proteobacteria
journal, June 2011

  • Hedrich, Sabrina; Schlömann, Michael; Johnson, D. Barrie
  • Microbiology, Vol. 157, Issue 6
  • DOI: 10.1099/mic.0.045344-0

Enigmatic, ultrasmall, uncultivated Archaea
journal, April 2010

  • Baker, B. J.; Comolli, L. R.; Dick, G. J.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 19
  • DOI: 10.1073/pnas.0914470107
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