Bacteria attenuation by iron electrocoagulation governed by interactions between bacterial phosphate groups and Fe(III) precipitates
Abstract
Iron electrocoagulation (Fe-EC) is a low-cost process in which Fe(II) generated from an Fe(0) anode reacts with dissolved O2 to form (1) Fe(III) precipitates with an affinity for bacterial cell walls and (2) bactericidal reactive oxidants. Previous work suggests that Fe-EC is a promising treatment option for groundwater containing arsenic and bacterial contamination. However, the mechanisms of bacteria attenuation and the impact of major groundwater ions are not well understood. In this work, using the model indicator Escherichia coli (E. coli), we show that physical removal via enmeshment in EC precipitate flocs is the primary process of bacteria attenuation in the presence of HCO3-, which significantly inhibits inactivation, possibly due to a reduction in the lifetime of reactive oxidants. Here, we demonstrate that the adhesion of EC precipitates to cell walls, which results in bacteria encapsulation in flocs, is driven primarily by interactions between EC precipitates and phosphate functional groups on bacteria surfaces. In single solute electrolytes, both P (0.4 mM) and Ca/Mg (1-13 mM) inhibited the adhesion of EC precipitates to bacterial cell walls, whereas Si (0.4 mM) and ionic strength (2-200 mM) did not impact E. coli attenuation. Interestingly, P (0.4 mM) did not affect E. coli attenuationmore »
- Authors:
-
- Univ. of California, Berkeley, CA (United States)
- Univ. of Utrecht (Netherlands)
- Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); United States Agency for International Development (USAID)
- OSTI Identifier:
- 1828154
- Alternate Identifier(s):
- OSTI ID: 1397363
- Grant/Contract Number:
- AC02-05CH11231; AID-OAA-A-13-00002
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Water Research
- Additional Journal Information:
- Journal Volume: 103; Journal ID: ISSN 0043-1354
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Iron electrocoagulation; Bacteria attenuation; Bacterial surface functional groups; Specific interactions; Bivalent cations; Oxyanions
Citation Formats
Delaire, Caroline, van Genuchten, Case M., Amrose, Susan E., and Gadgil, Ashok J. Bacteria attenuation by iron electrocoagulation governed by interactions between bacterial phosphate groups and Fe(III) precipitates. United States: N. p., 2016.
Web. doi:10.1016/j.watres.2016.07.020.
Delaire, Caroline, van Genuchten, Case M., Amrose, Susan E., & Gadgil, Ashok J. Bacteria attenuation by iron electrocoagulation governed by interactions between bacterial phosphate groups and Fe(III) precipitates. United States. https://doi.org/10.1016/j.watres.2016.07.020
Delaire, Caroline, van Genuchten, Case M., Amrose, Susan E., and Gadgil, Ashok J. Mon .
"Bacteria attenuation by iron electrocoagulation governed by interactions between bacterial phosphate groups and Fe(III) precipitates". United States. https://doi.org/10.1016/j.watres.2016.07.020. https://www.osti.gov/servlets/purl/1828154.
@article{osti_1828154,
title = {Bacteria attenuation by iron electrocoagulation governed by interactions between bacterial phosphate groups and Fe(III) precipitates},
author = {Delaire, Caroline and van Genuchten, Case M. and Amrose, Susan E. and Gadgil, Ashok J.},
abstractNote = {Iron electrocoagulation (Fe-EC) is a low-cost process in which Fe(II) generated from an Fe(0) anode reacts with dissolved O2 to form (1) Fe(III) precipitates with an affinity for bacterial cell walls and (2) bactericidal reactive oxidants. Previous work suggests that Fe-EC is a promising treatment option for groundwater containing arsenic and bacterial contamination. However, the mechanisms of bacteria attenuation and the impact of major groundwater ions are not well understood. In this work, using the model indicator Escherichia coli (E. coli), we show that physical removal via enmeshment in EC precipitate flocs is the primary process of bacteria attenuation in the presence of HCO3-, which significantly inhibits inactivation, possibly due to a reduction in the lifetime of reactive oxidants. Here, we demonstrate that the adhesion of EC precipitates to cell walls, which results in bacteria encapsulation in flocs, is driven primarily by interactions between EC precipitates and phosphate functional groups on bacteria surfaces. In single solute electrolytes, both P (0.4 mM) and Ca/Mg (1-13 mM) inhibited the adhesion of EC precipitates to bacterial cell walls, whereas Si (0.4 mM) and ionic strength (2-200 mM) did not impact E. coli attenuation. Interestingly, P (0.4 mM) did not affect E. coli attenuation in electrolytes containing Ca/Mg, consistent with bivalent cation bridging between bacterial phosphate groups and inorganic P sorbed to EC precipitates. Finally, we found that EC precipitate adhesion is largely independent of cell wall composition, consistent with comparable densities of phosphate functional groups on Gram-positive and Gram-negative cells. Our results are critical to predict the performance of Fe-EC to eliminate bacterial contaminants from waters with diverse chemical compositions.},
doi = {10.1016/j.watres.2016.07.020},
journal = {Water Research},
number = ,
volume = 103,
place = {United States},
year = {Mon Jul 11 00:00:00 EDT 2016},
month = {Mon Jul 11 00:00:00 EDT 2016}
}
Web of Science
Works referenced in this record:
Iron-Catalyzed Oxidation of Arsenic(III) by Oxygen and by Hydrogen Peroxide: pH-Dependent Formation of Oxidants in the Fenton Reaction
journal, June 2003
- Hug, Stephan J.; Leupin, Olivier
- Environmental Science & Technology, Vol. 37, Issue 12
Iron oxyhydroxide mineralization on microbial extracellular polysaccharides
journal, July 2009
- Chan, Clara S.; Fakra, Sirine C.; Edwards, David C.
- Geochimica et Cosmochimica Acta, Vol. 73, Issue 13
Effect of phosphate, silicate, and Ca on Fe(III)-precipitates formed in aerated Fe(II)- and As(III)-containing water studied by X-ray absorption spectroscopy
journal, January 2010
- Voegelin, Andreas; Kaegi, Ralf; Frommer, Jakob
- Geochimica et Cosmochimica Acta, Vol. 74, Issue 1
Multi-competitive interaction of As(III) and As(V) oxyanions with Ca2+, Mg2+, PO3−4, and CO2−3 ions on goethite
journal, April 2008
- Stachowicz, Monika; Hiemstra, Tjisse; van Riemsdijk, Willem H.
- Journal of Colloid and Interface Science, Vol. 320, Issue 2
ATR–FTIR Spectroscopic Investigation on Phosphate Adsorption Mechanisms at the Ferrihydrite–Water Interface
journal, September 2001
- Arai, Yuji; Sparks, D. L.
- Journal of Colloid and Interface Science, Vol. 241, Issue 2
ATR-FTIR Spectroscopy Study of the Influence of pH and Contact Time on the Adhesion of Shewanella putrefaciens Bacterial Cells to the Surface of Hematite
journal, November 2012
- Elzinga, Evert J.; Huang, Jen-How; Chorover, Jon
- Environmental Science & Technology, Vol. 46, Issue 23
Adsorption of phenol and substituted phenols by iron oxides
journal, April 1991
- McBride, M. B.; Kung, King-Hsi
- Environmental Toxicology and Chemistry, Vol. 10, Issue 4
ATR-FTIR Spectroscopy Reveals Bond Formation During Bacterial Adhesion to Iron Oxide
journal, September 2006
- Parikh, Sanjai J.; Chorover, Jon
- Langmuir, Vol. 22, Issue 20
Adhesion of Bacterial Exopolymers to α-FeOOH: Inner-Sphere Complexation of Phosphodiester Groups
journal, November 2004
- Omoike, Anselm; Chorover, Jon; Kwon, Kideok D.
- Langmuir, Vol. 20, Issue 25
Inactivation of Escherichia coli by Nanoparticulate Zerovalent Iron and Ferrous Ion
journal, September 2010
- Kim, Jee Yeon; Park, Hee-Jin; Lee, Changha
- Applied and Environmental Microbiology, Vol. 76, Issue 22
Role of Cell Surface Lipopolysaccharides in Escherichia c oli K12 Adhesion and Transport
journal, August 2004
- Walker, Sharon L.; Redman, Jeremy A.; Elimelech, Menachem
- Langmuir, Vol. 20, Issue 18
Adhesion of Shewanella oneidensis MR-1 to Goethite: A Two-Dimensional Correlation Spectroscopic Study
journal, April 2016
- Yan, Wei; Wang, Hongbo; Jing, Chuanyong
- Environmental Science & Technology, Vol. 50, Issue 8
ATR–FTIR spectroscopic evidence for biomolecular phosphorus and carboxyl groups facilitating bacterial adhesion to iron oxides
journal, July 2014
- Parikh, Sanjai J.; Mukome, Fungai N. D.; Zhang, Xiaoming
- Colloids and Surfaces B: Biointerfaces, Vol. 119
Hydrogen peroxide for prevention of bacterial growth on polymer biomaterials
journal, December 1999
- Alt, Eckhard; Leipold, Francesca; Milatovic, Danica
- The Annals of Thoracic Surgery, Vol. 68, Issue 6
Effects of pH on Bacterial Inactivation in Aqueous Solutions due to Low-Temperature Atmospheric Pressure Plasma Application: Effects of pH on Bacterial Inactivation in Aqueous Solutions …
journal, December 2009
- Ikawa, Satoshi; Kitano, Katsuhisa; Hamaguchi, Satoshi
- Plasma Processes and Polymers, Vol. 7, Issue 1
Fecal Indicator Bacteria Transport and Deposition in Saturated and Unsaturated Porous Media
journal, July 2012
- Chen, Gexin; Walker, Sharon L.
- Environmental Science & Technology, Vol. 46, Issue 16
Composition and structure of Fe(III)-precipitates formed by Fe(II) oxidation in water at near-neutral pH: Interdependent effects of phosphate, silicate and Ca
journal, August 2015
- Senn, Anna-Caterina; Kaegi, Ralf; Hug, Stephan J.
- Geochimica et Cosmochimica Acta, Vol. 162
Fe(III) Nucleation in the Presence of Bivalent Cations and Oxyanions Leads to Subnanoscale 7 Å Polymers
journal, October 2014
- van Genuchten, Case M.; Gadgil, Ashok J.; Peña, Jasquelin
- Environmental Science & Technology, Vol. 48, Issue 20
Escherichia coli Attenuation by Fe Electrocoagulation in Synthetic Bengal Groundwater: Effect of pH and Natural Organic Matter
journal, July 2015
- Delaire, Caroline; van Genuchten, Case M.; Nelson, Kara L.
- Environmental Science & Technology, Vol. 49, Issue 16
Adsorption of alpha amino acids at the water/goethite interface
journal, March 2008
- Norén, Katarina; Loring, John S.; Persson, Per
- Journal of Colloid and Interface Science, Vol. 319, Issue 2
Spectroscopic characterization of the structural and functional properties of natural organic matter fractions
journal, July 2002
- Chen, Jie; Gu, Baohua; LeBoeuf, Eugene J.
- Chemosphere, Vol. 48, Issue 1
Rate Constants for Reactions of Inorganic Radicals in Aqueous Solution
journal, July 1988
- Neta, P.; Huie, Robert E.; Ross, Alberta B.
- Journal of Physical and Chemical Reference Data, Vol. 17, Issue 3
Structure of the O antigen of Escherichia coli K-12 and the sequence of its rfb gene cluster
journal, July 1994
- Stevenson, G.; Neal, B.; Liu, D.
- Journal of Bacteriology, Vol. 176, Issue 13
Removing Arsenic from Synthetic Groundwater with Iron Electrocoagulation: An Fe and As K-Edge EXAFS Study
journal, December 2011
- van Genuchten, Case M.; Addy, Susan E. A.; Peña, Jasquelin
- Environmental Science & Technology, Vol. 46, Issue 2
A combined electrochemical-irradiation treatment of highly colored and polluted industrial wastewater
journal, July 2003
- Barrera-Dı́az, C.; Ureña-Nuñez, F.; Campos, E.
- Radiation Physics and Chemistry, Vol. 67, Issue 5
Lipopolysaccharide Density and Structure Govern the Extent and Distance of Nanoparticle Interaction with Actual and Model Bacterial Outer Membranes
journal, August 2015
- Jacobson, Kurt H.; Gunsolus, Ian L.; Kuech, Thomas R.
- Environmental Science & Technology, Vol. 49, Issue 17
Analysis of Bacterial Deposition on Metal (Hydr)oxide-Coated Sand Filter Media
journal, July 1998
- Truesdail, S. E.; Lukasik, J.; Farrah, S. R.
- Journal of Colloid and Interface Science, Vol. 203, Issue 2
Proton and metal adsorption onto bacterial consortia: Stability constants for metal–bacterial surface complexes
journal, April 2007
- Johnson, Kelly J.; Szymanowski, Jennifer E. S.; Borrok, David
- Chemical Geology, Vol. 239, Issue 1-2
Arsenic(III, V) adsorption on a goethite-based adsorbent in the presence of major co-existing ions: Modeling competitive adsorption consistent with spectroscopic and molecular evidence
journal, April 2013
- Kanematsu, Masakazu; Young, Thomas M.; Fukushi, Keisuke
- Geochimica et Cosmochimica Acta, Vol. 106
The carbonate radical and related oxidants derived from bicarbonate buffer
journal, January 2007
- Medinas, Danilo B.; Cerchiaro, Giselle; Trindade, Daniel F.
- IUBMB Life, Vol. 59, Issue 4
Virulence and Immunogenic Activities of B. Typhosus in Relation to its Antigenic Constituents
journal, August 1935
- Felix, A.; Pitt, R. M.
- Journal of Hygiene, Vol. 35, Issue 3
A universal surface complexation framework for modeling proton binding onto bacterial surfaces in geologic settings
journal, June 2005
- Borrok, D.
- American Journal of Science, Vol. 305, Issue 6-8
The future for electrocoagulation as a localised water treatment technology
journal, April 2005
- Holt, Peter K.; Barton, Geoffrey W.; Mitchell, Cynthia A.
- Chemosphere, Vol. 59, Issue 3
Modeling As(III) Oxidation and Removal with Iron Electrocoagulation in Groundwater
journal, October 2012
- Li, Lei; van Genuchten, Case M.; Addy, Susan E. A.
- Environmental Science & Technology, Vol. 46, Issue 21
Ionic Strength Effects on Silicic Acid (H 4 SiO 4 ) Sorption and Oligomerization on an Iron Oxide Surface: An Interesting Interplay between Electrostatic and Chemical Forces
journal, October 2011
- Dol Hamid, Rossuriati; Swedlund, Peter J.; Song, Yantao
- Langmuir, Vol. 27, Issue 21
Influence of Phosphate on Bacterial Adhesion onto Iron Oxyhydroxide in Drinking Water
journal, February 2002
- Appenzeller, Brice M. R.; Duval, Yann B.; Thomas, Fabien
- Environmental Science & Technology, Vol. 36, Issue 4
Mechanisms of virus control during iron electrocoagulation – Microfiltration of surface water
journal, May 2012
- Tanneru, Charan Tej; Chellam, Shankararaman
- Water Research, Vol. 46, Issue 7
Structure of Fe(III) precipitates generated by the electrolytic dissolution of Fe(0) in the presence of groundwater ions
journal, February 2014
- van Genuchten, Case M.; Peña, Jasquelin; Amrose, Susan E.
- Geochimica et Cosmochimica Acta, Vol. 127
Application of electrocoagulation in Escherichia coli culture and two surface waters
journal, January 2008
- Ghernaout, D.; Badis, A.; Kellil, A.
- Desalination, Vol. 219, Issue 1-3
Reactions of the ferryl ion with some compounds found in cloud water
journal, January 1998
- Jacobsen, Frank; Holcman, Jerzy; Sehested, Knud
- International Journal of Chemical Kinetics, Vol. 30, Issue 3
Chemical Force Spectroscopy Evidence Supporting the Layer-by-Layer Model of Organic Matter Binding to Iron (oxy)Hydroxide Mineral Surfaces
journal, August 2015
- Chassé, Alexander W.; Ohno, Tsutomu; Higgins, Steven R.
- Environmental Science & Technology, Vol. 49, Issue 16
Arsenic Removal with Iron(II) and Iron(III) in Waters with High Silicate and Phosphate Concentrations
journal, January 2004
- Roberts, Linda C.; Hug, Stephan J.; Ruettimann, Thomas
- Environmental Science & Technology, Vol. 38, Issue 1
Engineering Solutions to Improve the Removal of Fecal Indicator Bacteria by Bioinfiltration Systems during Intermittent Flow of Stormwater
journal, June 2013
- Mohanty, Sanjay K.; Torkelson, Andrew A.; Dodd, Hanna
- Environmental Science & Technology, Vol. 47, Issue 19
Factors Affecting the Yield of Oxidants from the Reaction of Nanoparticulate Zero-Valent Iron and Oxygen
journal, February 2008
- Keenan, Christina R.; Sedlak, David L.
- Environmental Science & Technology, Vol. 42, Issue 4
Comparison of the acid-base behaviour and metal adsorption characteristics of a gram-negative bacterium with other strains
journal, April 2003
- Ngwenya, Bryne T.; Sutherland, Ian W.; Kennedy, Lynn
- Applied Geochemistry, Vol. 18, Issue 4
Electro-chemical arsenic remediation: Field trials in West Bengal
journal, August 2014
- Amrose, Susan E.; Bandaru, Siva R. S.; Delaire, Caroline
- Science of The Total Environment, Vol. 488-489
Arsenic removal in synthetic ground water using iron electrolysis
journal, February 2014
- Li, Lei; Li, Jun; Shao, Chen
- Separation and Purification Technology, Vol. 122
Works referencing / citing this record:
Bacterial bioreporter detection of arsenic associated with iron oxides
journal, January 2018
- van Genuchten, Case M.; Finger, Amanda; van der Meer, Jan R.
- Environmental Science: Processes & Impacts, Vol. 20, Issue 6
Field demonstration of solar-powered electrocoagulation water treatment system for purifying groundwater contaminated by both total coliforms and arsenic
journal, June 2019
- Oh, Chamteut; Pak, Seungil; Han, Young-Soo
- Environmental Technology, Vol. 42, Issue 3
Electrocoagulation as a Pretreatment for Electroxidation of E. coli
journal, November 2019
- Lynn, William; Heffron, Joe; Mayer, Brooke K.
- Water, Vol. 11, Issue 12
Electrodisinfection of real swine wastewater for water reuse
journal, July 2018
- Simas, Angélica; Mores, Rúbia; Steffens, Juliana
- Environmental Chemistry Letters, Vol. 17, Issue 1
Field demonstration of solar-powered electrocoagulation water treatment system for purifying groundwater contaminated by both total coliforms and arsenic
text, January 2019
- Oh, Chamteut; Pak, Seungil; Han, Young-Soo
- Taylor & Francis
Field demonstration of solar-powered electrocoagulation water treatment system for purifying groundwater contaminated by both total coliforms and arsenic
text, January 2019
- Oh, Chamteut; Pak, Seungil; Han, Young-Soo
- Taylor & Francis