Engineering Corynebacterium glutamicum to produce the biogasoline isopentenol from plant biomass hydrolysates
Abstract
Abstract Background Many microbes used for the rapid discovery and development of metabolic pathways have sensitivities to final products and process reagents. Isopentenol (3-methyl-3-buten-1-ol), a biogasoline candidate, has an established heterologous gene pathway but is toxic to several microbial hosts. Reagents used in the pretreatment of plant biomass, such as ionic liquids, also inhibit growth of many host strains. We explored the use of Corynebacterium glutamicum as an alternative host to address these constraints. Results We found C. glutamicum ATCC 13032 to be tolerant to both the final product, isopentenol, as well to three classes of ionic liquids. A heterologous mevalonate-based isopentenol pathway was engineered in C. glutamicum . Targeted proteomics for the heterologous pathway proteins indicated that the 3-hydroxy-3-methylglutaryl-coenzyme A reductase protein, HmgR, is a potential rate-limiting enzyme in this synthetic pathway. Isopentenol titers were improved from undetectable to 1.25 g/L by combining three approaches: media optimization; substitution of an NADH-dependent HmgR homolog from Silicibacter pomeroyi ; and development of a C. glutamicum ∆poxB ∆ldhA host chassis. Conclusions We describe the successful expression of a heterologous mevalonate-based pathway in the Gram-positive industrial microorganism, C. glutamicum, for the production of the biogasoline candidate, isopentenol. We identified critical genetic factors to harnessmore »
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1618759
- Alternate Identifier(s):
- OSTI ID: 1545316
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Published Article
- Journal Name:
- Biotechnology for Biofuels
- Additional Journal Information:
- Journal Name: Biotechnology for Biofuels Journal Volume: 12 Journal Issue: 1; Journal ID: ISSN 1754-6834
- Publisher:
- Springer Science + Business Media
- Country of Publication:
- Netherlands
- Language:
- English
- Subject:
- 09 BIOMASS FUELS
Citation Formats
Sasaki, Yusuke, Eng, Thomas, Herbert, Robin A., Trinh, Jessica, Chen, Yan, Rodriguez, Alberto, Gladden, John, Simmons, Blake A., Petzold, Christopher J., and Mukhopadhyay, Aindrila. Engineering Corynebacterium glutamicum to produce the biogasoline isopentenol from plant biomass hydrolysates. Netherlands: N. p., 2019.
Web. doi:10.1186/s13068-019-1381-3.
Sasaki, Yusuke, Eng, Thomas, Herbert, Robin A., Trinh, Jessica, Chen, Yan, Rodriguez, Alberto, Gladden, John, Simmons, Blake A., Petzold, Christopher J., & Mukhopadhyay, Aindrila. Engineering Corynebacterium glutamicum to produce the biogasoline isopentenol from plant biomass hydrolysates. Netherlands. https://doi.org/10.1186/s13068-019-1381-3
Sasaki, Yusuke, Eng, Thomas, Herbert, Robin A., Trinh, Jessica, Chen, Yan, Rodriguez, Alberto, Gladden, John, Simmons, Blake A., Petzold, Christopher J., and Mukhopadhyay, Aindrila. Wed .
"Engineering Corynebacterium glutamicum to produce the biogasoline isopentenol from plant biomass hydrolysates". Netherlands. https://doi.org/10.1186/s13068-019-1381-3.
@article{osti_1618759,
title = {Engineering Corynebacterium glutamicum to produce the biogasoline isopentenol from plant biomass hydrolysates},
author = {Sasaki, Yusuke and Eng, Thomas and Herbert, Robin A. and Trinh, Jessica and Chen, Yan and Rodriguez, Alberto and Gladden, John and Simmons, Blake A. and Petzold, Christopher J. and Mukhopadhyay, Aindrila},
abstractNote = {Abstract Background Many microbes used for the rapid discovery and development of metabolic pathways have sensitivities to final products and process reagents. Isopentenol (3-methyl-3-buten-1-ol), a biogasoline candidate, has an established heterologous gene pathway but is toxic to several microbial hosts. Reagents used in the pretreatment of plant biomass, such as ionic liquids, also inhibit growth of many host strains. We explored the use of Corynebacterium glutamicum as an alternative host to address these constraints. Results We found C. glutamicum ATCC 13032 to be tolerant to both the final product, isopentenol, as well to three classes of ionic liquids. A heterologous mevalonate-based isopentenol pathway was engineered in C. glutamicum . Targeted proteomics for the heterologous pathway proteins indicated that the 3-hydroxy-3-methylglutaryl-coenzyme A reductase protein, HmgR, is a potential rate-limiting enzyme in this synthetic pathway. Isopentenol titers were improved from undetectable to 1.25 g/L by combining three approaches: media optimization; substitution of an NADH-dependent HmgR homolog from Silicibacter pomeroyi ; and development of a C. glutamicum ∆poxB ∆ldhA host chassis. Conclusions We describe the successful expression of a heterologous mevalonate-based pathway in the Gram-positive industrial microorganism, C. glutamicum, for the production of the biogasoline candidate, isopentenol. We identified critical genetic factors to harness the isopentenol pathway in C. glutamicum . Further media and cultivation optimization enabled isopentenol production from sorghum biomass hydrolysates.},
doi = {10.1186/s13068-019-1381-3},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 12,
place = {Netherlands},
year = {Wed Feb 27 00:00:00 EST 2019},
month = {Wed Feb 27 00:00:00 EST 2019}
}
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https://doi.org/10.1186/s13068-019-1381-3
https://doi.org/10.1186/s13068-019-1381-3
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