Title: Toxicogenomic responses of Caenorhabditis elegans to pristine and transformed zinc oxide nanoparticles

Abstract

Manufactured nanoparticles (MNPs) undergo transformation immediately after they enter wastewater treatment streams and during their partitioning to sewage sludge, which is applied to agricultural soils in form of biosolids. We examined toxicogenomic responses of the model nematode Caenorhabditis elegans to pristine and transformed ZnO-MNPs (phosphatized pZnO- and sulfidized sZnO-MNPs). To account for the toxicity due to dissolved Zn, a ZnSO₄ treatment was included. Transformation of ZnO-MNPs reduced their toxicity by nearly ten-fold, while there was almost no difference in the toxicity of pristine ZnO-MNPs and ZnSO₄. This combined with the fact that far more dissolved Zn was released from ZnO- compared to pZnO- or sZnO-MNPs, suggests that dissolution of pristine ZnO-MNPs is one of the main drivers of their toxicity. Transcriptomic responses at the EC₃₀ for reproduction resulted in a total of 1161 differentially expressed genes. Fifty percent of the genes differentially expressed in the ZnSO₄ treatment, including the three metal responsive genes (mtl-1, mtl-2 and numr-1), were shared among all treatments, suggesting that responses to all forms of Zn could be partially attributed to dissolved Zn. However, the toxicity and transcriptomic responses in all MNP treatments cannot be fully explained by dissolved Zn. Two of the biological pathways identified,more » one essential for protein biosynthesis (Aminoacyl-tRNA biosynthesis) and another associated with detoxification (ABC transporters), were shared among pristine and one or both transformed ZnO-MNPs, but not ZnSO₄. When comparing pristine and transformed ZnO-MNPs, 66% and 40% of genes were shared between ZnO-MNPs and sZnO-MNPs or pZnO-MNPs, respectively. This suggests greater similarity in transcriptomic responses between ZnO-MNPs and sZnO-MNPs, while toxicity mechanisms are more distinct for pZnO-MNPs, where 13 unique biological pathways were identified. Based on these pathways, the toxicity of pZnO-MNPs is likely to be associated with their adverse effect on digestion and metabolism.« less

Authors:

Starnes, Daniel  ^[1];

• Search DOE PAGES for author "Starnes, Daniel"
• Search DOE PAGES for ORCID "0000-0002-1603-4999"

Unrine, Jason  ^[2];

• Search DOE PAGES for author "Unrine, Jason"
• Search DOE PAGES for ORCID "0000-0003-3012-5261"

Chen, Chun ^[3]; Lichtenberg, Stuart ^[2]; Starnes, Catherine ^[4]; Svendsen, Claus ^[5]; Kille, Peter  ^[6];

• Search DOE PAGES for author "Kille, Peter"
• Search DOE PAGES for ORCID "0000-0001-6023-5221"

Morgan, John ^[6]; Baddar, Zeinah Elhaj ^[2]; Spear, Amanda ^[2]; Bertsch, Paul ^[7]; Chen, Kuey Chu ^[2]; Tsyusko, Olga ^[2]

---

+ Show Author Affiliations

1. Univ. of Kentucky, Lexington, KY (United States); Belmont University, Nashville, TN (United States)
2. Univ. of Kentucky, Lexington, KY (United States)
3. Univ. of Kentucky, Lexington, KY (United States); Northwest A&F University Shaanxi (China)
4. Belmont University, Nashville, TN (United States); Univ. of Kentucky, Lexington, KY (United States)
5. Centre for Ecology and Hydrology (United Kingdom)
6. Cardiff University (United Kingdom)
7. Commonwealth Scientific and Industrial Research Organization (CSIRO), Brisbane, QLD (Australia)

Publication Date:

Tue Jan 29 00:00:00 EST 2019

Research Org.:

University of Chicago, IL (United States); Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Org.:

USDOE Office of Science (SC), Basic Energy Sciences (BES)

OSTI Identifier:

1610189

Alternate Identifier(s):

OSTI ID: 1636354

Grant/Contract Number:  

FG02-92ER14244; AC02-98CH10886

Resource Type:

Accepted Manuscript

Journal Name:

Environmental Pollution

Additional Journal Information:

Journal Volume: 247; Journal ID: ISSN 0269-7491

Publisher:

Elsevier

Country of Publication:

United States

Language:

English

Subject:

54 ENVIRONMENTAL SCIENCES; gene expression; nanomaterial; nematode; transcriptomics; soil; wastewater